Just started my phlebotomy course and have been feeling a bit overwhelmed. I have found your channel to be extremely helpful!! Thank you. My stress level is slowly going down. :)
I'm an RN,I found your video very helpful. We don't always have a detailed in-service like this we're just shown the basics and away we go. Pick up what you need as you go along. So thanks for this video. See you all in the trenches....you are appreciated.
I did an online phlebotomy class that was three weeks.I was going crazy not understanding this. 3 months later (today) this finally makes sense! Thank yoooou!!
This is the best video on the order of draw! Just started my MLT program and was very nervous. I’m also taking phlebotomy now. Your videos are extremely helpful. Thank you!!
This was awesome! I’m prepping for a job interview tomorrow after completing my certification 5 months ago, so I needed a refresher on the OOD, in case I’m asked a question about it. Very helpful resource to use along with my notes. Thank you SO much for this! ☺️ *new subscriber*
Our teacher AND BOOK (Phlebotomy Essentials 6th edition by McCall and Tankersley) says the OOD is blood cultures, yellow, light blue, red, gold, light green, green, lavender, then, gray. It's so difficult learning OOD when so many people do it differently. I cant find one video of the way we do it and my teacher is terrible.
Sorry for your confusion! Going just by the color can be an issue. Yes, we try to make it easy and visual by making the phlebotomy rainbow but the order is based on the additive...which is based on the kind of specimen characteristics needed for the desired test. Your text is the same one I use for class! ; ) The best way to study is to know why the specific additive will give you the desired specimen. For example, it would not make sense to use a SST (gold serum separator tube) for a CBC because separating the cells from the liquid portion of the blood would give falsely elevated counts. The other part that you need to understand is how contamination of the additive may affect the next tube. The biggest offender here is the EDTA contamination of the SST. The way that EDTA binds to Calcium is a very strong bond. During testing of a contaminated chemistry profile (SST tube), that EDTA will bind the calcium so that the level will be critically low. Since, Potassium EDTA is the anticoagulant of choice...the Poatassium would also be falsely elevated/critical. Another consideration is the body's response to the wound created by the venipuncture. Clotting is going to get initiated almost immediately. That is why the Blue tops, used for coagulation testing, are drawn first if blood cultures are not needed. Factor levels ould not be accurate if the blue tops were drawn at the end. Please check out the link in the video description if you would like more info about the correct order of draw. Also, make sure you go by what the course text says. Focus on the name of the additive, the color, the type of specimen created by the tube (whole blood, plasma, or serum), and the types of tests measured from the tube.
My teacher is horrible too, that's why I'm here on RUclips learning this stuff all by myself because the teacher hasn't done any teaching yet & I've been in this class for 5 weeks. I hope your class goes well for you.
Thank you so much for this video, I just started a job at a lab and needed a good refresher! Very good info here! You’re a life saver. Is there a video about separating serum from cmp to prepare for lab pick up!?
@@MedicalLabLadyGill I have figured out the centrifuging. But about separating serum do you use the same pipette per person ? Also do you put multiple serum from let’s say 3 sst tubes into one transport tube or does it have to be separated into individual transport tubes?
Fig aliquoting a specimen needs to be performed with one transfer pipette per sample. Each patient tube should have its own receiving tube. The venipuncture collection tubes each have a different additive and blood from them should never be mixed. I am still guessing about your original question. If you are in accessioning and are delivering tubes to the bench, the SST should be centrifuged and delivered to that bench. If you're in accessioning and are preparing the tubes to be sent to a reference lab your facility usually has instructions on how to do this (they might expect phlebotomy to draw an extra tube just in case their is an add-on test ordered later in house). Usually, you can send the original collection tube or put the plasma/serum in one of the tubes provided by the reference lab.
Very important steps she miss. You have to fill the blue tube all the way up to the line. If it is the first vial because you are not drawing blood cultures, you need to use a red tube first just to fill the butterfly needle tubing up with blood then draw the blue. If you don’t the blue tube will get air in it first then there will not be the required ratio of blood to anticoagulant and you would have to redraw.
@@andriennevalendebelen9618 yes, there is a line on the blue top tube that marks the appropriate fill so the coagulation test results are not adversely affected. Evey additive tube needs to be filled to a certain amount so the specimen to additive ratio is appropriate for testing.
This is an extremely helpful video for my classes. I tell my students to pay close attention to what happens if you draw the lavender before the others. Scary.
Thank you for your support!!! I am working on a collaboration between nursing, HIM, and lab students to talk about the effects that situation has on a patient. It should make for a really great discussion AND bring about more awareness of the issue. : )
Tron TwoProgram That is true. I think I was trying to convey that you may not always be drawing both (or any blood cultures for that matter) but said it in a poor way. Thank you for pointing this out ; )
I was about to say that. I agree with you. The bottles she is showing need a butterfly because they do not fit a normal vacutainer holder. We used to get these in a kit from LabCorp.
Yellow is anticoagulant, infections blood cultures. Light blue coagulation, PT/INR, Red is plain so it's chemistry draws Light green is lithium heparin PST so lactate dark green sodium heparin is ammonia and then lavender or purple edta cbc diff gray potassium oxalate sodium fluoride for like blood alcohol and glucose
Congratulations!!! The main rule is to know the additives and ideally the testing performed on them. Some of the colors are arbitrary and can be confusing. For example, you can review several comments on this video from individuals telling me I filmed the wrong order of draw because yellow is first in a textbook and on the BD site. If one looks at the additive listed it is for a blood culture bottle and should not be confused with the Gold Serum Separator Tube (SST) used for the main chemistries. A general rule of thumb is to draw Blood Culture Bottles first (if needed), Light Blue (coagulation testing because venipuncture initiates clotting right away), a STOP LIGHT Red Gold Green (used for various chemistries), Lavender and Pink (EDTA tubes for Hematology & Blood Banking), Gray (fasting glucose) That is the quickest way I could put that all together. Hope it will be helpful! Knowing what's in the tube and why it is used for testing will help you beyond measure. Please make sure to take the time to know them inside and out. Each of your patients and coworkers will be grateful!
@@zuley6957 images.app.goo.gl/Ee8DWPwCixA8pN5f9 this is one hospital's chart that includes order of draw and testing performed. Also, check out the BD website. Google "BD order of draw" and you can see the proper order of draw for regular and microtainers. PLEASE make sure to memorize by additive!!!!
I’m confused when some one comes to the lab to get blood drawn how do you know what tubes too use as in what is the easiest way to know what tube to use and if your using the right one
@@rosaliedeleon629 Their doctor should order specific tests. There's also a basic blood work set and if nothing else is ordered then you'd just do that.
If you use a butterfly you will spend vacuun for the blue tubes and they will take less blood. In that case you must use the red tubes first to absorb the vacuum leak and then the blue to be filled with the correct amount of ml so that the anticoagulant agent dilution is correct.
Hey I am a new subscriber and I’ve been watching your videos for about a few days now and I am so happy I found you. I am not sure if you did a video on this yet, but how do I know what color to use when it comes to test tube color look up for drawing blood from a doctor order?
That is a great question! There are differences among institutions regarding their policies & instrumentation for certain tests. As a whole, BD has great resources on which tubes are used for various tests. You can find that information here www.acllaboratories.com/static/documents/KV1259_ACL_BD_Tube_Chart_11.2020.pdf
My teacher has it as: 1. yellow tops (sos) 2. blood culture bottles (culture media) 3. Yellow (ACD) 4. light blue 5. Red/grey tiger top 6. gold top 7. Red top 8. Light green 9. Dark green 10. Lavender 11. Pink top 12. White top 13. Gray top. Does this sound about right?!
With the little blue tubes... you said the anticoagulant removes the calcium... but then the lab techs just add some calcium back into the specimen when its in the lab. Why does it have to be removed in the first place then? I'm rather new to this, I appologize in advance if its a common sense answer.
That is a really great question! Thank you for asking! An anticoagulant is a tube additive which stops the process of clotting in the blood sample. Clotting happens because of a long chain reaction (clotting cascade) of various proteins and elements (they are generally called coagulation/coag factors because they need to be present in order for clotting to happen) present in the blood. This chain reaction interacts with the platelets, also known as thrombocytes, to form a clot. One of the major coag factor is calcium. If an anticoagulant, such as Sodium citrate (light blue top tube) or EDTA (lavender or pink top tube), binds to calcium present in the tube...that calcium is no longer available to take part in the clotting cascade. This makes it so the chemical and cellular composition of the blood sample can stay similar to the way it is in the patient's blood at the time of the venipuncture. The lab's job is to try and mimic what is happening in the patient so we can provide answers to the physician about the patient's health status. Calcium is a component used in the testing reagents of the coagulation test because at that point, we want the blood to clot. The length of time it takes for the blood to clot can tell a laboratorian A LOT about the patient's health status. Length of time can indicate an increase or shortage of platelets or coag factors, the presence of platelets or coag factors that don't work correctly, the presence of various medications, etc.
Ohhh..... so in the lab, they want to watch how long it takes the blood to clot by adding their own calcium. Thank you so much for this info, it has helped alot!
so basically it all depends wat facilty u work into,, basically gold and green tubes can be used either or i mean for chemistry but green is basically for stat test.
Question: What if I drew just the red, tiger, and yellow top out of order, does it affect the results? Also, very informative and straight forward, thank you. I'm starting my new job and I am studying over my notes from school and youtubing it up! 🙂
Pr0D16y Thank you for the question! I am not really sure that you would run into that kind of situation because they can be used for the same types of tests (chemistry). They are all made to provide serum by forcing the blood to clot. Therefore, if the occasion did arise and these three ONLY were not in the correct order among themselves there would probably not be a problem. But again, it is highly unlikely that you would experience this issue.
The reason you draw reds before SSTs is because some reds are glass and have no clot activator in them. If you draw that after the SST, the blood will clot faster than it's supposed to in a glass red. So drawing red before sst helps to commit the GLASS red to memory.
Thanks for the educational video! I'm wondering what age did you graduate, because most Medical Doctors graduate in the United States by age 19. Good work! Let freedom ring!
Thank you for your support! Medical Doctors graduate medical school in their 20's or 30's depending on when they started (immediately after undergraduate, after a graduate degree, or after waiting in between 2 degrees). I am not sure how it is outside of the USA.
I often see people draw the lithium or EDTA tube (if also ordered) before the citrate tube to “get the air out” instead of using a discard. How might this affect the results? They would draw green, blue lavender or sometimes lavender, blue no other tubes were ordered.
That is interesting. I haven't seen that before. EDTA contamination is usually of concern in chemistry tubes but it could also affect the coagulation results during testing. EDTA has a stronger bond to calcium than that of the blue top's sodium citrate. EDTA could falsely elevate/lengthen the clotting time. The best practice would be to use 2 blues at the beginning with the first as a discard tube like you referenced in you comment.
Potassium EDTA is the additive in both the purple and pink top venipuncture tubes. It binds to calcium in the blood to stop the blood from clotting. If the order of draw is not followed correctly during venipuncture, and the EDTA tube is collected before the gold serum separator tube (SST), it can cause erroneous chemistry results. The potassium, from the additive, causes a elevated/often critical potassium chemistry level. It also binds to the calcium in the SST and causes a critically low (if even measurable) calcium level. This is a QA issue known as EDTA contamination and the chemistry results should not be reported. There should be investigation about the phlebotomy procedure. Staff should be notified of the need for correct order of draw for accuracy. The order should then be canceled and reordered & recollected if still needed.
We use phlebotomy essentials in my program however you can also find the information about venipuncture and capillary puncture collection order from the BD website. Book on Amazon www.amazon.com/Phlebotomy-Essentials-Enhanced-Ruth-McCall/dp/1284209946/ref=mp_s_a_1_3?adgrpid=74214589141&gclid=CjwKCAjwq5-WBhB7EiwAl-HEkl_N3ePVj2cEAKfYy_KJtWyWqVvktQ18G5kMB5G6gmd90VMXV2SO9RoC7Q0QAvD_BwE&hvadid=379319255666&hvdev=m&hvlocphy=9007687&hvnetw=g&hvqmt=e&hvrand=533472703185616698&hvtargid=kwd-824017562338&hydadcr=14831_10218892&keywords=phlebotomy+essentials+7th+edition&qid=1657281623&sr=8-3 BD website www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection
How long should it take for the blood to clot in the red tube that has the silica in it? Is it like 30 minutes? What if someones blood clotted in less than 5 is that bad?
Thank you so much for these informative videos Please, if you can tell me if there any book for all tests procedure steps. In need to know or if you sugest abook
That is a really challenging question. The manufacturers of the tests create procedures for them. Many tests have similar procedures no matter what company created them. Textbooks and professional societies also contain procedures. Are you asking about Phlebotomy specifically or the lab in general?
Let’s say you have to do a blood draw for two different tests but they’re for the same colored tube. Do you have to use two separate tubes or can you use one tube for both tests?
Thank you for the questions! Venipuncture can include just one or two tubes, however, it should be performed by trained personnel. If you are interested in the benefits of PRP I suggest you make an appointment with your physician. He or she can then discuss the appropriate procedure if that treatment is right for you. Please do not try performing needle sticks on yourself or anyone else without proper training.
The best thing to know for the correct order of draw is the additive of the blood collection container. Yellow comes first in some renditions but the additive is SPS. In these cases, yellow stands for the blood culture bottle.
Just started my phlebotomy course and have been feeling a bit overwhelmed. I have found your channel to be extremely helpful!! Thank you. My stress level is slowly going down. :)
I am so glad!
I just started mine Monday! This is my first video, can't wait for it to start after reading this comment!!
@@SomeOne-zw6kh I start mine last week
Hi. Hello is this validated worldwide. I'm from the uk
Hi I start my phlebotomy course this Saturday, how did it go for you?? I’m getting nervous. What do you think I need to pass/complete the class?
Just took my NH exam and passed with a score of 420. Thank you for this detailed video, could not have done it without your help
I got 410 nice job 👍 I have interview tomorrow needed a refresher course 😗 wish me luck 🍀
@@mikeperez5986 you got this, buddy!
Best of luck!
I start my phlebotomy training on the 24th ! I’m nervous and excited.. tips please lol
I’m taking my NHA exam for CPT & CET Nov 10th wish me luck your video is very helpful.
@@mikeperez5986 hello can you please tell me what’s all that’s on the NHA test getting ready to take the test and I’m so scared
Starting school for phlebotomy in a few weeks. Trying to get a leg up before class. Great information. The best so far I’ve seen on RUclips.
I'm an RN,I found your video very helpful. We don't always have a detailed in-service like this we're just shown the basics and away we go. Pick up what you need as you go along. So thanks for this video. See you all in the trenches....you are appreciated.
So glad to help and thank you!!!! We do love & value all of our healthcare team members ♡
This was very educational. The perfect video for me to touch up my knowledge before I take my state exam next month
I did an online phlebotomy class that was three weeks.I was going crazy not understanding this. 3 months later (today) this finally makes sense! Thank yoooou!!
I am so glad! Congratulations and welcome to the lab family!!!!!
I am u today. Hopefully i will understand better
What online class was that ??
I watched your video many times it helped me to pass my NHA exam at first time. Thank you very much.❤❤❤❤❤
STARTING SCHOOL IN TWO WEEKS AND THIS IS VERY INFORMAL. THANKS
Just finished NHA exam and passed for phlebotomy. Yippie.
CONGRATULATIONS 🤗🥳🥳🥳🥳 Welcome to the family!!!
This is the best order of draw video I've found, thank you!
This video is a life saver for me who just started a phlebotomy course
Games of Yesterday n Today Let's Plays & More hi what are the rewuirements to take a phlebotomy course?
I am starting my phlebotomy class on February 15th, 2012
This is the best video on the order of draw! Just started my MLT program and was very nervous. I’m also taking phlebotomy now. Your videos are extremely helpful. Thank you!!
Thank you for your support! Enjoy your program, that's exciting!
Did you get your phlebotomy certificate?
Blood cultures 0:55
Light Blue 4:08
Red 5:12
SST 6:05
Green 7:40
Lavender 8:50
Gray 14:18
What is this
@raju The order in which tubes need to be filled and the time it is in the video
I heard ppl say that red should be last due to you want it to clot
Marion Rhodoleia that’s why you want it to be one of the first ones because you don’t want a additive from any other tub to stop that process
@@GrippysocksCC would using a vacutainer change The Order of draw?
STARTED MY PHLEBOTOMY COURSE 2 WEEKS AGO , THIS VIDEO HELPED ME ALOT 😉
Congratulations!!! Hope you have a great time and learn a lot!!!
Excellent! The best video that I have seen that thoroughly explains the tubes in detail. Thank you!
Thank you for your support!
I would really enjoy learning the test that go with each tube
This was awesome! I’m prepping for a job interview tomorrow after completing my certification 5 months ago, so I needed a refresher on the OOD, in case I’m asked a question about it. Very helpful resource to use along with my notes. Thank you SO much for this! ☺️ *new subscriber*
Thank you so much for your support! Glad it was helpful! Good luck tomorrow!!!!
How did your interview go? Where you asked about the drawing of blood order?
Thank you for this, in the same boat myself this morning
Thank you . Very informative. Also, you have a lovely voice. You would make a great radio host.
Thank you very much!
This video helped me a lot on my phlebotomy skills. Thank you for making this helpful video!
Nurse who appreciates the in-depth and thoughtful explanations
So glad to have you! Thank you for your support!
Personally, your right on target...thank you very very much, concise and clear.
Glad it was helpful.
I just completed last week and I'm very anxious. Praying to find a job quickly so I can get more experience to build the confidence
Congratulations and all the best! Phlebotomists are in high demand and are a very important/critical part of patient care.
@Medical Lab Lady Gill thankyou. What helps with nerves?? I'm so nervous. Praying I find a job to help me build my confidence
Perfect, the best explanation that I found. Thank you
Thank you for your support! Glad I could help!
thank you so much for putting in the detailed information 🙏🏻 it helps a lot
You explained this si well !!!
Thx a bunch 😊
Our teacher AND BOOK (Phlebotomy Essentials 6th edition by McCall and Tankersley) says the OOD is blood cultures, yellow, light blue, red, gold, light green, green, lavender, then, gray. It's so difficult learning OOD when so many people do it differently. I cant find one video of the way we do it and my teacher is terrible.
Sorry for your confusion! Going just by the color can be an issue. Yes, we try to make it easy and visual by making the phlebotomy rainbow but the order is based on the additive...which is based on the kind of specimen characteristics needed for the desired test.
Your text is the same one I use for class! ; ) The best way to study is to know why the specific additive will give you the desired specimen. For example, it would not make sense to use a SST (gold serum separator tube) for a CBC because separating the cells from the liquid portion of the blood would give falsely elevated counts.
The other part that you need to understand is how contamination of the additive may affect the next tube. The biggest offender here is the EDTA contamination of the SST. The way that EDTA binds to Calcium is a very strong bond. During testing of a contaminated chemistry profile (SST tube), that EDTA will bind the calcium so that the level will be critically low. Since, Potassium EDTA is the anticoagulant of choice...the Poatassium would also be falsely elevated/critical.
Another consideration is the body's response to the wound created by the venipuncture. Clotting is going to get initiated almost immediately. That is why the Blue tops, used for coagulation testing, are drawn first if blood cultures are not needed. Factor levels ould not be accurate if the blue tops were drawn at the end.
Please check out the link in the video description if you would like more info about the correct order of draw. Also, make sure you go by what the course text says. Focus on the name of the additive, the color, the type of specimen created by the tube (whole blood, plasma, or serum), and the types of tests measured from the tube.
Here's the link education.bd.com/images/view.aspx?productId=1537
That is so true we learn it a different way blood cultures, red then blue
My teacher is horrible too, that's why I'm here on RUclips learning this stuff all by myself because the teacher hasn't done any teaching yet & I've been in this class for 5 weeks. I hope your class goes well for you.
@@amandaclover9191
This is so disheartening. When you say the teacher is not teaching can you give an example of what you feel is missing in class?
This was a SUPER helpful refresher! Thank you SO MUCH!!
You're welcome! Thank you for your support!
Great and informative video! Thank you for sharing.
ann jean
Thank you for your comment!
Thank you so much for this video, I just started a job at a lab and needed a good refresher! Very good info here! You’re a life saver. Is there a video about separating serum from cmp to prepare for lab pick up!?
Thank you for your support! Glad to help!
Do you mean centrifuging the specimen or aliquoting as well (separation of serum)?
@@MedicalLabLadyGill I have figured out the centrifuging. But about separating serum do you use the same pipette per person ? Also do you put multiple serum from let’s say 3 sst tubes into one transport tube or does it have to be separated into individual transport tubes?
Fig aliquoting a specimen needs to be performed with one transfer pipette per sample. Each patient tube should have its own receiving tube. The venipuncture collection tubes each have a different additive and blood from them should never be mixed.
I am still guessing about your original question. If you are in accessioning and are delivering tubes to the bench, the SST should be centrifuged and delivered to that bench. If you're in accessioning and are preparing the tubes to be sent to a reference lab your facility usually has instructions on how to do this (they might expect phlebotomy to draw an extra tube just in case their is an add-on test ordered later in house). Usually, you can send the original collection tube or put the plasma/serum in one of the tubes provided by the reference lab.
Very important steps she miss. You have to fill the blue tube all the way up to the line. If it is the first vial because you are not drawing blood cultures, you need to use a red tube first just to fill the butterfly needle tubing up with blood then draw the blue. If you don’t the blue tube will get air in it first then there will not be the required ratio of blood to anticoagulant and you would have to redraw.
is that what they call the "full draw"? i'm having problems in looking for the meaning of full draw.
The explanation of the blue top fill is in another video.
@@andriennevalendebelen9618 yes, there is a line on the blue top tube that marks the appropriate fill so the coagulation test results are not adversely affected. Evey additive tube needs to be filled to a certain amount so the specimen to additive ratio is appropriate for testing.
No. Use a GLASS red(no additive) or another blue.
This is an extremely helpful video for my classes. I tell my students to pay close attention to what happens if you draw the lavender before the others. Scary.
Thank you for your support!!! I am working on a collaboration between nursing, HIM, and lab students to talk about the effects that situation has on a patient. It should make for a really great discussion AND bring about more awareness of the issue. : )
Hi all the best regards u have up
I thought if you use a butterfly you should draw aerobic first because there is air in the butterfly tube , ? What do you think
Tron TwoProgram
That is true. I think I was trying to convey that you may not always be drawing both (or any blood cultures for that matter) but said it in a poor way. Thank you for pointing this out ; )
You are right and you will always draw both
@@MissSunDaiRose Not always
I was about to say that. I agree with you. The bottles she is showing need a butterfly because they do not fit a normal vacutainer holder. We used to get these in a kit from LabCorp.
You use a Discard tube 1st when using the butterfly to get the air out of the tube .
Really helpful for my upcoming Blood Science exam! Thank you so much :)
Glad I could help!
just follow your professor instruction.
One of the most helpful videos Thank you!!!
Best video great explanation
Thank you was worried about this part as I didn’t know if the color of tube order take test in four days
I needed clarification about how you went back and forth on explaining different tubes.
I'm sooooo glad I found this video very helpful information thank you
Glad to help!
WOW. THAT was a REALLY Great video!!!
Thank you for your support!
Great video. Very helpful. Can you go over the tests hat would go with the different tubes? Thank you!
Great question!
Yellow is anticoagulant, infections blood cultures. Light blue coagulation, PT/INR, Red is plain so it's chemistry draws Light green is lithium heparin PST so lactate dark green sodium heparin is ammonia and then lavender or purple edta cbc diff gray potassium oxalate sodium fluoride for like blood alcohol and glucose
Oops and gold/tiger top SST for routine chemistry
LOVE YOUR VIDEO! Breaks down the order of draw so flawlessly, i feel very confident for my finals comp tomorrow thank you.
The culture bottles DO have to be aerobic -> anaerobic IF the line you're drawing from hasn't been purged of air first.
isn't the red supposed to come before blue since it's no additive and u would want to prevent any additive from getting into it?
That’s what I learned!
Thanks a lot for all these useful information,I appreciate that 💛☮️🌻🙃🐻
Thanks for the information ℹ️
Great Video
Thank you very much for the great video it's really good and you did explain it very well!!
it would be so much easier if they made the order in the rainbow formation!
Great video ❤❤❤
I start my program in the fall. is there any tips and tricks for studying order of draw and remembering the colors?
Congratulations!!!
The main rule is to know the additives and ideally the testing performed on them. Some of the colors are arbitrary and can be confusing. For example, you can review several comments on this video from individuals telling me I filmed the wrong order of draw because yellow is first in a textbook and on the BD site. If one looks at the additive listed it is for a blood culture bottle and should not be confused with the Gold Serum Separator Tube (SST) used for the main chemistries.
A general rule of thumb is to draw Blood Culture Bottles first (if needed), Light Blue (coagulation testing because venipuncture initiates clotting right away), a STOP LIGHT Red Gold Green (used for various chemistries), Lavender and Pink (EDTA tubes for Hematology & Blood Banking), Gray (fasting glucose)
That is the quickest way I could put that all together. Hope it will be helpful! Knowing what's in the tube and why it is used for testing will help you beyond measure. Please make sure to take the time to know them inside and out. Each of your patients and coworkers will be grateful!
Now the additives and Lab Uses -
@@zuley6957 images.app.goo.gl/Ee8DWPwCixA8pN5f9 this is one hospital's chart that includes order of draw and testing performed. Also, check out the BD website. Google "BD order of draw" and you can see the proper order of draw for regular and microtainers. PLEASE make sure to memorize by additive!!!!
I’m confused when some one comes to the lab to get blood drawn how do you know what tubes too use as in what is the easiest way to know what tube to use and if your using the right one
@@rosaliedeleon629 Their doctor should order specific tests.
There's also a basic blood work set and if nothing else is ordered then you'd just do that.
Great informative video! Thank you!!!
Thank you for your support!
great video please keep posting
Thanks you so much lady Gill you have really taught me a lesson
Thank you for your support!
If you use a butterfly you will spend vacuun for the blue tubes and they will take less blood. In that case you must use the red tubes first to absorb the vacuum leak and then the blue to be filled with the correct amount of ml so that the anticoagulant agent dilution is correct.
I recently found your channel its been very helpful especially i liked blood bank videos.
And i hope you do blood banking videos again. Thank you.
Thank you for your support! Welcome to the family! We are getting new equipment for the fall semester and I hope to be doing more videos.
Awesome teaching thank you!!!
Hey I am a new subscriber and I’ve been watching your videos for about a few days now and I am so happy I found you. I am not sure if you did a video on this yet, but how do I know what color to use when it comes to test tube color look up for drawing blood from a doctor order?
That is a great question! There are differences among institutions regarding their policies & instrumentation for certain tests. As a whole, BD has great resources on which tubes are used for various tests. You can find that information here www.acllaboratories.com/static/documents/KV1259_ACL_BD_Tube_Chart_11.2020.pdf
That is so interesting how doctors do that that is so amazing
My teacher has it as:
1. yellow tops (sos)
2. blood culture bottles (culture media)
3. Yellow (ACD)
4. light blue
5. Red/grey tiger top
6. gold top
7. Red top
8. Light green
9. Dark green
10. Lavender
11. Pink top
12. White top
13. Gray top.
Does this sound about right?!
it okay just a slight difference with what my teacher gave out. He combined grey tiger,gold and orange as one.
Thank you for this video... very informative
Im a medical assistant student will be in clinical in June starting to get ready for ncct certification
Very nicely explained. Thank you.
Just perfect 👍
I start on the 21st. Somewhat terrified. A little excited... but pretty nervous
Remi wtf Same here
THANK YOU THANK YOU THANK YOU!! SOOOO VERY VERY HELPFUL!
You're welcome!
With the little blue tubes... you said the anticoagulant removes the calcium... but then the lab techs just add some calcium back into the specimen when its in the lab. Why does it have to be removed in the first place then? I'm rather new to this, I appologize in advance if its a common sense answer.
That is a really great question! Thank you for asking!
An anticoagulant is a tube additive which stops the process of clotting in the blood sample. Clotting happens because of a long chain reaction (clotting cascade) of various proteins and elements (they are generally called coagulation/coag factors because they need to be present in order for clotting to happen) present in the blood. This chain reaction interacts with the platelets, also known as thrombocytes, to form a clot. One of the major coag factor is calcium.
If an anticoagulant, such as Sodium citrate (light blue top tube) or EDTA (lavender or pink top tube), binds to calcium present in the tube...that calcium is no longer available to take part in the clotting cascade. This makes it so the chemical and cellular composition of the blood sample can stay similar to the way it is in the patient's blood at the time of the venipuncture. The lab's job is to try and mimic what is happening in the patient so we can provide answers to the physician about the patient's health status. Calcium is a component used in the testing reagents of the coagulation test because at that point, we want the blood to clot. The length of time it takes for the blood to clot can tell a laboratorian A LOT about the patient's health status. Length of time can indicate an increase or shortage of platelets or coag factors, the presence of platelets or coag factors that don't work correctly, the presence of various medications, etc.
Ohhh..... so in the lab, they want to watch how long it takes the blood to clot by adding their own calcium. Thank you so much for this info, it has helped alot!
Exactly! Glad to help!
I'm also a perfossional phlebotomist with 12 years of experience
Thnx
Great job 👌
incredible video thank you
Glad to help!
I'm very confused, i just watch 3 different video for the order of draw and it's 3 different order... Help some please🙌😳
Christine Johnson am literally having the same problem so glad am not alone smh . Am suppose to take the NHA test the 9th Of December 🤦♀️
nadege king how did it go for you? I hope well!
Check out the BD website for the order of draw. They have great pocket aids as well! They are the standard used by most facilities and texts.
so basically it all depends wat facilty u work into,, basically gold and green tubes can be used either or i mean for chemistry but green is basically for stat test.
@@MedicalLabLadyGill what is BD website ?
Question: What if I drew just the red, tiger, and yellow top out of order, does it affect the results?
Also, very informative and straight forward, thank you. I'm starting my new job and I am studying over my notes from school and youtubing it up! 🙂
Pr0D16y
Thank you for the question! I am not really sure that you would run into that kind of situation because they can be used for the same types of tests (chemistry). They are all made to provide serum by forcing the blood to clot. Therefore, if the occasion did arise and these three ONLY were not in the correct order among themselves there would probably not be a problem. But again, it is highly unlikely that you would experience this issue.
How can I you pauple top
The reason you draw reds before SSTs is because some reds are glass and have no clot activator in them. If you draw that after the SST, the blood will clot faster than it's supposed to in a glass red. So drawing red before sst helps to commit the GLASS red to memory.
Where is the dark green tube?
great video, clear explanation... thanks so much
Thank you for your support!
Good video thank you sister
Thank you for your support!
Very informative, thank you!
Nina Gomez
You're welcome!
My exam is on June 26th 2020. Thank you for passing this information on
Hope you did well!
@@MedicalLabLadyGill I passed!!!
@@cameryne2352 YES!!!!!!!!! CONGRATULATIONS!!!!!!!!!!
@@MedicalLabLadyGill :)))))
@@cameryne2352 how was it? Was it hard?
That looked cool
really helpful and subscribed!!
Awesome! Thank you for your support!
This is so helpful, thank you
You're welcome!
Thanks for your video what about the yellow tube and the blue one?
Very informative nicely done
Thank you!
Thanks for the educational video! I'm wondering what age did you graduate, because most Medical Doctors graduate in the United States by age 19. Good work! Let freedom ring!
Thank you for your support! Medical Doctors graduate medical school in their 20's or 30's depending on when they started (immediately after undergraduate, after a graduate degree, or after waiting in between 2 degrees). I am not sure how it is outside of the USA.
bro 19? wtf are you talking about. not everyone is doogie howser lol
What????? Who do u know that graduated at age 19?? Gtfoh
Thank you so much
Thank you for your support!
I often see people draw the lithium or EDTA tube (if also ordered) before the citrate tube to “get the air out” instead of using a discard. How might this affect the results? They would draw green, blue lavender or sometimes lavender, blue no other tubes were ordered.
That is interesting. I haven't seen that before. EDTA contamination is usually of concern in chemistry tubes but it could also affect the coagulation results during testing. EDTA has a stronger bond to calcium than that of the blue top's sodium citrate. EDTA could falsely elevate/lengthen the clotting time. The best practice would be to use 2 blues at the beginning with the first as a discard tube like you referenced in you comment.
Thank you 😊
Going to be honest I'm a bit confused about the light purple draw order and the issue it causes
Potassium EDTA is the additive in both the purple and pink top venipuncture tubes. It binds to calcium in the blood to stop the blood from clotting. If the order of draw is not followed correctly during venipuncture, and the EDTA tube is collected before the gold serum separator tube (SST), it can cause erroneous chemistry results. The potassium, from the additive, causes a elevated/often critical potassium chemistry level. It also binds to the calcium in the SST and causes a critically low (if even measurable) calcium level. This is a QA issue known as EDTA contamination and the chemistry results should not be reported. There should be investigation about the phlebotomy procedure. Staff should be notified of the need for correct order of draw for accuracy. The order should then be canceled and reordered & recollected if still needed.
Good tutorial thanks alot
Hello
Thank you for this video.
Do you have any book for me to review all this
We use phlebotomy essentials in my program however you can also find the information about venipuncture and capillary puncture collection order from the BD website.
Book on Amazon www.amazon.com/Phlebotomy-Essentials-Enhanced-Ruth-McCall/dp/1284209946/ref=mp_s_a_1_3?adgrpid=74214589141&gclid=CjwKCAjwq5-WBhB7EiwAl-HEkl_N3ePVj2cEAKfYy_KJtWyWqVvktQ18G5kMB5G6gmd90VMXV2SO9RoC7Q0QAvD_BwE&hvadid=379319255666&hvdev=m&hvlocphy=9007687&hvnetw=g&hvqmt=e&hvrand=533472703185616698&hvtargid=kwd-824017562338&hydadcr=14831_10218892&keywords=phlebotomy+essentials+7th+edition&qid=1657281623&sr=8-3
BD website www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection
I thought that an Ammonia level had to be drawn into an EDTA tube then kept on ice. Is the lithium heparin the same?
Ammonia should be drawn in a Lithium heparin (green top) tube and kept on ice. The anticoagulants do not react with the blood the same way.
this is super useful
How long should it take for the blood to clot in the red tube that has the silica in it? Is it like 30 minutes? What if someones blood clotted in less than 5 is that bad?
Thank you so much for these informative videos
Please, if you can tell me if there any book for all tests procedure steps.
In need to know or if you sugest abook
That is a really challenging question. The manufacturers of the tests create procedures for them. Many tests have similar procedures no matter what company created them. Textbooks and professional societies also contain procedures. Are you asking about Phlebotomy specifically or the lab in general?
Thank you for replying. I'm asking about general lab
@@revolution4u ok, then my reply still stands. I hope it's helpful.
This helped soooo much thank you!
So glad to help!
Let’s say you have to do a blood draw for two different tests but they’re for the same colored tube. Do you have to use two separate tubes or can you use one tube for both tests?
Megan Obrien It usually depends on the amount of blood you need for each test
Thanks
which tube type would you use for PRP TREATMENTS?
Can i use just one? Which one for PRP? Do you have to use the red one with butterfly? Is it safe to draw my own blood or could i die?
Thank you for the questions!
Venipuncture can include just one or two tubes, however, it should be performed by trained personnel. If you are interested in the benefits of PRP I suggest you make an appointment with your physician. He or she can then discuss the appropriate procedure if that treatment is right for you. Please do not try performing needle sticks on yourself or anyone else without proper training.
Good learning
why does yellow sometimes go before blood culture's ,
The best thing to know for the correct order of draw is the additive of the blood collection container. Yellow comes first in some renditions but the additive is SPS. In these cases, yellow stands for the blood culture bottle.
May i use your videos for my youtube video to describe any topic in hindi language?