I just started doing TEM. The video is just amazing!!!! A wealth of information in here. Very crisp presentation. Thanks to this video, I could follow everything during my hands-on training. Thank you!!!!
Thank you for the comment and I'm glad you found the video helpful! If you have any questions or requests for topics you would like me to cover in a future video, please let me know.
Hi Nicholas your tutorials are very insightful and explained very well. It's very useful for us, wish you upload more such tutorials on TEM covering topics n techniques like dark field imaging, nano beam diffraction, EELS, dislocation, stacking fault analysis etc.
Hi Biraj: I actually do have videos on dark-field and nano beam diffraction on my channel! I don't have an EELS video yet because the system doesn't currently have EELS. Thank you for the suggestion for a fault analysis video, I think that would be a good addition to the channel.
Great series of movies! I love the hands-on demonstrations! This was a real life saver in my microscopy class, as we couldn't go to the lab for a demonstration experiment due COVID-19 restrictions.
Thanks for your valuable videos, I want to know the function of turbopumps and what happens when the button turbo on opened and closed also I want to know the concept of the airlock
Hi Mostafa: thanks; the turbo pumps are part of the vacuum system and can have various functions depending on the particular part of the vacuum system, but in general, the function of a turbo pump is to get a section of the vacuum system to the point of high vacuum (around 10E-6 Torr range); the point of the airlock is to allow the specimen holder to be introduced into the column without having to also introduce a large volume of air into the column, which would likely cause a vacuum system crash. Another reason the turbo pumps are useful is that they are dry and use no oil so they help keep hydrocarbons from being introduced into the column, which can be detrimental to imaging. Pretty much all modern S/TEM vacuum systems are now oil-free.
Hi Nicholas, I did not understand the use of rotation centre alignment. What shall happen if we do not do rotation centre alignment? Rotation centre alignment seems to be similar to eucentric height alignment?
Hi Sudipta: sorry for the late reply. Rotation centering is critical to get the best resolution from your objective lens. Depending on how badly uncorrected it is, not performing rotation centering may result in a blurry image with loss of fine details (particularly atomic-resolution levels of detail). Setting eucentric height is likely more important that performing rotation centering; your objective lens (if properly configured) is optimized to properly focus with the sample at eucentric height; if you deviate too far from eucentric height, you deviate far from the optimized setting for your objective lens to get a properly focused image and this too may result in loss of detail (resolution). All in all, both rotation centering and setting eucentric height are very important in getting the best performance, but my opinion is that setting eucentric height is the more critical element.
Hi Aravinda: sorry for the late reply. I have a couple videos on my channel about performing selected area diffraction and recording diffraction patterns, both with the Tecnai and the Talos. Please go take a look at those and feel free to leave me a comment/question if you need additional information.
Thank you for the nice video. What do you use to recenter the beam after finishing adjusting Astigmatism @19:35, Beam tilt pp X/ Beam tilt pp Y @20:53, Rotation center @22:30, a different Mag@33:03? Is it the trackball or beam shift?
HI DW; glad you like the video! Yes, each time I am just using the dedicated beam shift trackball on the left hand panel. Some people prefer to use the MF knobs with the beam shift function but I like the ball since is always dedicated for this.
Hi CECCO: great question! Without going into too much detail, minimum contrast = minimum focus is related to all "rays" (electrons) emanating from the sample being focused to as small an area as possible; if this area is excessive large, there is more contrast. I hope this helps.
@@NicholasRudawski Thanks for your quick reply . Another question please How can I know I am in centric height or not because I can't find the difference in image before and after your correction eccentric height (only difference is the array of square ) and how the eccentric height is related to the array of squares on support film?
@@mostafaeid9955 There are two main methods to set eucentric height; this can be done by wobbling the alpha tilt axis back and forth and then adjusting stage Z to minimize image shifting or it can be done by setting your objective lens to a known preset value (eucentric focus) and adjusting stage Z until the image is in focus. The first method is the more accurate way to do this, but the second method can be faster and easier to perform. Provided the "eucentric focus" setting is correct, there really should not be much difference between the two methods in the final result. If you look through my channel, I actually have a video that specifically talks about eucentric height so that may be good to take a look at.
Hi Nicholas, your videos are of great help. Thanks for making it. I would like to know what is the difference between shifting the beam using trackball in LHP vs shifting the beam using beamshift MF X/Y knob? thanks in advance.
Hi Nikhil: glad you find the videos helpful. There really isn't any difference; you can configure the MF knobs to do the same thing as the trackball, but since the trackball is always dedicated for shifting and the MF knobs are multifunctional it just makes more sense (at least to me) to just use the trackball for beam shifting.
Ha ha, nothing very fancy; I just used the video capture on my iphone 7; in my later videos, I discarded the audio from the videos then later recorded overdubbed audio on my computer using moviemaker for better audio.
@@NicholasRudawski I see, thanks Nicholas. I am always looking for some wearable devices that can record my experiment just like google Glass. But sometimes just using our phones is more convenient hahaha.
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Thank you for this information. This quality of scientific content on RUclips is rare.
Hi Corbin: thanks for the kind words; I'm always happy to hear that my videos are helping people out.
I just started doing TEM. The video is just amazing!!!! A wealth of information in here.
Very crisp presentation. Thanks to this video, I could follow everything during my hands-on training.
Thank you!!!!
Thank you for the comment and I'm glad you found the video helpful! If you have any questions or requests for topics you would like me to cover in a future video, please let me know.
Your videos and narrations are so good that it feels as if I am handling the instrument with you! Thank you.
Hi Debalaya: thank you, I'm glad you find the videos helpful.
Hi Nicholas your tutorials are very insightful and explained very well. It's very useful for us, wish you upload more such tutorials on TEM covering topics n techniques like dark field imaging, nano beam diffraction, EELS, dislocation, stacking fault analysis etc.
Hi Biraj: I actually do have videos on dark-field and nano beam diffraction on my channel! I don't have an EELS video yet because the system doesn't currently have EELS. Thank you for the suggestion for a fault analysis video, I think that would be a good addition to the channel.
It's a nice video and I like it so much!
Thank you!
This is a great video! Thank you, Dr. Nicholas.
Great series of movies! I love the hands-on demonstrations! This was a real life saver in my microscopy class, as we couldn't go to the lab for a demonstration experiment due COVID-19 restrictions.
Thank you for uploading such quality content
You're welcome; thanks for stopping by to watch!
You are my hero...Thank you for your great effort to make this video.
Thank you!.
This video is really helpful, it contains every detail I was looking for, thank you a lot for this!
You're welcome, I'm glad you found it useful.
Thank you for this video. Amazing explanation
Thank you for sharing the knowledge.
You're welcome.
Very nice explanation 👍
Thank you
Thanks for your valuable videos, I want to know the function of turbopumps and what happens when the button turbo on opened and closed also I want to know the concept of the airlock
Hi Mostafa: thanks; the turbo pumps are part of the vacuum system and can have various functions depending on the particular part of the vacuum system, but in general, the function of a turbo pump is to get a section of the vacuum system to the point of high vacuum (around 10E-6 Torr range); the point of the airlock is to allow the specimen holder to be introduced into the column without having to also introduce a large volume of air into the column, which would likely cause a vacuum system crash. Another reason the turbo pumps are useful is that they are dry and use no oil so they help keep hydrocarbons from being introduced into the column, which can be detrimental to imaging. Pretty much all modern S/TEM vacuum systems are now oil-free.
Very great effort!! Thank you for this informative video..
Thank you! More videos showing operation of the instrument in different ways will be forthcoming.
Thank you. This is very helpful
You're welcome, I'm glad you found this helpful!
Hi Nicholas, I did not understand the use of rotation centre alignment. What shall happen if we do not do rotation centre alignment? Rotation centre alignment seems to be similar to eucentric height alignment?
Hi Sudipta: sorry for the late reply. Rotation centering is critical to get the best resolution from your objective lens. Depending on how badly uncorrected it is, not performing rotation centering may result in a blurry image with loss of fine details (particularly atomic-resolution levels of detail). Setting eucentric height is likely more important that performing rotation centering; your objective lens (if properly configured) is optimized to properly focus with the sample at eucentric height; if you deviate too far from eucentric height, you deviate far from the optimized setting for your objective lens to get a properly focused image and this too may result in loss of detail (resolution). All in all, both rotation centering and setting eucentric height are very important in getting the best performance, but my opinion is that setting eucentric height is the more critical element.
Hi Nicholas , please make a video on TEM alignments to get good diffraction pattern ,
Hi Aravinda: sorry for the late reply. I have a couple videos on my channel about performing selected area diffraction and recording diffraction patterns, both with the Tecnai and the Talos. Please go take a look at those and feel free to leave me a comment/question if you need additional information.
Thank you very helpful
You're welcome, I'm glad you found this helpful.
Thank you a lot, it is very helpful :)
You're welcome, I'm glad you found it useful.
Thank you!
You're welcome; sorry for the very late reply!
Nice 👍
Thank you.
Thanks for the great work! Will you do more in the future? Maybe setting up the two-beam condition, or weak-beam dark field imaging demo?
Thank you! Yes, a video will be forthcoming covering two-beam imaging and weak-beam dark-field imaging.
Nicholas Rudawski You rock!
Hello, Nicholas, I wanna download your video. Do you have link ? Many thanks
Thank you for the nice video. What do you use to recenter the beam after finishing adjusting Astigmatism @19:35, Beam tilt pp X/ Beam tilt pp Y @20:53, Rotation center @22:30, a different Mag@33:03? Is it the trackball or beam shift?
HI DW; glad you like the video! Yes, each time I am just using the dedicated beam shift trackball on the left hand panel. Some people prefer to use the MF knobs with the beam shift function but I like the ball since is always dedicated for this.
Hi Nicholas. Just one question, why the sample (in TEM imaging) is in focus when we have the lowest contrast? Thanks.
Hi CECCO: great question! Without going into too much detail, minimum contrast = minimum focus is related to all "rays" (electrons) emanating from the sample being focused to as small an area as possible; if this area is excessive large, there is more contrast. I hope this helps.
is objective aperture is inserted in imaging or diffraction mode?
Hi Mostafa: usually, the objective aperture is inserted in imaging mode and retracted in diffraction mode (with rare exceptions).
@@NicholasRudawski Thanks for your quick reply . Another question please How can I know I am in centric height or not because I can't find the difference in image before and after your correction eccentric height (only difference is the array of square ) and how the eccentric height is related to the array of squares on support film?
@@mostafaeid9955 There are two main methods to set eucentric height; this can be done by wobbling the alpha tilt axis back and forth and then adjusting stage Z to minimize image shifting or it can be done by setting your objective lens to a known preset value (eucentric focus) and adjusting stage Z until the image is in focus. The first method is the more accurate way to do this, but the second method can be faster and easier to perform. Provided the "eucentric focus" setting is correct, there really should not be much difference between the two methods in the final result. If you look through my channel, I actually have a video that specifically talks about eucentric height so that may be good to take a look at.
Hi Nicholas, your videos are of great help. Thanks for making it. I would like to know what is the difference between shifting the beam using trackball in LHP vs shifting the beam using beamshift MF X/Y knob? thanks in advance.
Hi Nikhil: glad you find the videos helpful. There really isn't any difference; you can configure the MF knobs to do the same thing as the trackball, but since the trackball is always dedicated for shifting and the MF knobs are multifunctional it just makes more sense (at least to me) to just use the trackball for beam shifting.
@@NicholasRudawski Great! Thanks a lot!
@@nikhilpokharel Sure thing!
When inserting the holder, better to hold the single tilt holder and let it move in gentally into the microscope. Otherwise, the air vacuum may crash!
Hi Nicholas, I would like to ask what kind of equipment did you use to record this video?
Ha ha, nothing very fancy; I just used the video capture on my iphone 7; in my later videos, I discarded the audio from the videos then later recorded overdubbed audio on my computer using moviemaker for better audio.
@@NicholasRudawski I see, thanks Nicholas. I am always looking for some wearable devices that can record my experiment just like google Glass. But sometimes just using our phones is more convenient hahaha.
Go Gators!🐊
Go Gators!