Haemoglobin Estimation- Sahli's Method (हीमोग्लोबिन टेस्ट) By Solution Pharmacy
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- Опубликовано: 20 окт 2024
- This video is also available in ENGLISH. Estimation of Haemoglobin by Sahli's Method (ENGLISH) By Solution Pharmacy • Estimation of Haemoglo...
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Haemoglobin is the major component of blood which carries oxygen to the tissue for various function. when that goes down below the normal level one feels lethargy and fatigue. it's always better to estimate the haemoglobin level in blood.
After watching this video you will be able to understand- what is haemoglobin test, haemoglobin experiment, haemoglobin in Hindi, Sahli's hemoglobinometer, how to check haemoglobin in blood, haemoglobin estimation test, find your haemoglobin count, haemoglobin level in man, haemoglobin level in the woman,
Haemoglobin can be estimated by two methods-
Sahli's Haemoglobinometer and haemoglobin colour scale.
Step by steps procedure to estimate haemoglobin in blood by Sahli's method.
1- Take out the Haemoglobinometer and arrange its component in a stable place.
2.Prepare 0.1N HCl
3.Fill the 0.1 N into Sahli Haemometer tube up to 2% mark
4. Now place the pipette in Sahli Haemometer at a given place (in between two windows)
5. Now sterilize the fingertip and prick it with the help of sterilized lancet and fill the blood into Sahli Haemometer pipette up to 20 microliter marks.
6.Now transfer that blood sample into Sahli Haemometer tube in which we have already taken 0.1 N HCl.
7.Wait for about 10 minutes to let the reaction occur.
8.Now slowly-slowly dropwise add distilled water to its side and shake well with the help of given glass rod.
9.Keep adding distilled water till the colour of blood match the side window
10. Observe and note down the reading, that’s the percentage of haemoglobin
हेमोग्लोबिन रक्त का प्रमुख घटक है जो विभिन्न कार्यों के लिए ऊतक को ऑक्सीजन लेता है। जब यह सामान्य स्तर से नीचे चला जाता है तो कोई सुस्त और थकान महसूस करता है। रक्त में हीमोग्लोबिन स्तर का अनुमान लगाने के लिए हमेशा बेहतर होता है।
इस वीडियो को देखने के बाद आप समझ पाएंगे- हेमोग्लोबिन परीक्षण, हेमोग्लोबिन प्रयोग, हिंदी में हीमोग्लोबिन, सहली के हीमोग्लोबिनोमीटर, रक्त में हीमोग्लोबिन की जांच कैसे करें, हीमोग्लोबिन अनुमान परीक्षण, अपने हीमोग्लोबिन गिनती, हेमोग्लोबिन स्तर में आदमी, हीमोग्लोबिन स्तर औरत,
हेमोग्लोबिन का अनुमान दो तरीकों से किया जा सकता है-
सहली के हीमोग्लोबिनोमीटर और हीमोग्लोबिन रंग पैमाने।
साहली की विधि द्वारा रक्त में हीमोग्लोबिन का अनुमान लगाने के लिए कदम प्रक्रियाओं के चरण।
1- हेमोग्लोबिनोमीटर निकालें और इसके घटक को स्थिर स्थान पर व्यवस्थित करें।
2. 0.1 एन एचसीएल तैयार करें
3. 0.1 एन को सहली हैमोमीटर ट्यूब में 2% तक भरें
4. अब दिए गए स्थान पर सहली हैमोमीटर में पिपेट रखें (दो खिड़कियों के बीच में)
5. अब उंगलियों को निर्जलित करें और नसबंदी वाले लेंस की मदद से इसे छेड़छाड़ करें और रक्त को 20 माइक्रोलिटर अंकों तक सहली हैमोमीटर पिपेट में भरें।
6. अब हस्तांतरण करें कि सहली हैमोमीटर ट्यूब में रक्त नमूना जिसमें हम पहले ही 0.1 एन एचसीएल ले चुके हैं।
7. प्रतिक्रिया होने के लिए लगभग 10 मिनट के लिए प्रतीक्षा करें।
8. धीरे धीरे धीरे-धीरे ड्रॉपवाइड अपनी तरफ आसुत पानी जोड़ें और दी गई ग्लास रॉड की मदद से अच्छी तरह से हिलाएं।
9. खून के रंग से तरफ खिड़की से मेल खाने तक आसुत पानी डालना रखें
10. पढ़ना और पढ़ना नोट करें, यह हीमोग्लोबिन का प्रतिशत है
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Kon kon.meri trh practical se ak din phle ye video dekhne aaye h 😂😂
Mera to aaj hi he 🤣
@@chandnivishwakarma5889 all the best
Yes
Mein 😅
Yup 😅🥵
Thank you so much. I have done this practical in my class but completely forgotten the process your video helped me to recall the process.
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I understand Very easy how you forgot
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I really really can't thank you enough for making this video, Prayers from core of my heart Sir.
Seham Baloch Thank you for your comment. We are happy that we could help you. Keep supporting solution
For God's and your own sake, learn the correct method from somewhere else.
Just a note: The stirrer should be lifted when comparing the colors, but not completely taken out of the tube. It's so as to avoid any potential loss of fluid which would lead to a lower Hb estimate.
Yes you are right. We did a slight mistake here. Sorry for that
The explanation is good... But we need to wait for atleast 10min after adding blood to n/10hcl .. For formation of acid haematin
Yes you are right. I forgot to tell this point . Thank you
Yeah 😊
Exactly
@@solutionpharmacy but govt hospital me 10 sec me hi result dere h
Tq sir.....kal mera phyology ka prectical he....aapka video dekhne ke Baad ab full confidence aa gya ....tq once again sir
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Tq..sir
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sir 1 year proof mbbs ka seprate alag video bna dijiyea
Thank u sir.... tomorrow's my physio practical exam
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17 gram aa Sakta he
Same here
😁path practicle here
Thank you so much. It was so helpful Ans very easy!!! Tmrw is my practical and you literally saved my time!!
Thank you for your kind feedback
Doing this experiment before 30 min of viva... That's called backbenchers...
And getting knowledge on top level by @solution pharmacy..
@solution pharmacy s padhne ka ghamand hai hamko😎... Sir sirf Pharmaceutics ya Pharmaceutical chemistry ki liye bhi lecture karwa do.
Dr,sahab aap ka bahut bahut bahut dhanyawaad
Thank u sir for giving perfect explanation tommarrow I have practical exam I think will right well
Jack hacker All the best
Thanks a lot
After mixing blood with acid ,,wait for 10min and then add distilled water drop by drop
Yes
Yes
Nice pic
Right bro
nice ans.
Exam ke ek din phle wale bachee sbse achee 😂😂😂
Alhamdulleh
aapne meri bahut help ki mujhe sare process samjh aa gye thanks a lot sir🥰♥️
My pleasure
The finger should not be squeezed to take out the blood, otherwise tissue fluid might come out,thus giving the lower estimation of haemoglobin
Yeah but most of the cases we have to squeeze the finger to get enough blood at 1 time otherwise bubble will produce which will largely effect it and the blood in capillary tube may clot in no time.
When we performed in lab most of students had to squeeze it or have to prick at different place on finger to get enough blood. With a tiny prick
Not squeezing but can gently press
right sir
This man doesn't know this simple fact and he doesn't care.
Ye sab kitabo me sahi hai , practically nahi😅
Mona here from pakistan.it is so usefull to understand experiment without confusion.
Thank you so much sir... this video is really helpful for me..... specially the details given in the description box..... 👍👍..... inshallah I will do my best in this experiment...
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It helped me in my practical exam ,(mbbs),thanks
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Thank u so much sir aapki vjah se meri itni bdi tension duur ho gai actually kl mera zoology practical ka exam h or mein yeh test krna bhul gaii thi ab mere ko bhi ache se smjh aa gya or mein isko kl easily lab mein Mr skti hu ty again
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Haematology ki zoology😅
Thank you, Proff practical exam ki din video dekh raha hoon
Thanku so much sir ur video giving me a confidence that I'll do my best in my practical class
My pleasure. Thank you for feedback. All the best
Got to know another way to fill the capillary tube thank you
Very good video....so much useful for first year MBBS students
10k subscribers without any video thank you
Sir microscope ke bare me bataiye im dmlt 2year students run..
Sir thank you apka to samjya Hua pura samaj me agaya
Bahut achhi baat hai ye. Thank you for feedback
Thank you so much sir for giving us so much information details about this practical.
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Thank you so much sir🙏
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Tq uu sir very useful for our examination
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Kon kon practical ke time hi dekh rha hai
icann't i understand Hb test before
but now i know very well
thanku sir
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Please made such more videos on the other Pharmaceutical Practical topic....
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Just before practical 👁️👁️ thanks
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Kon kon meri tarah practical ke hee din ye video dekhne aya h😂😉
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Very nice, aj me v ki hu lab me, sir ne same aisa hi btaya
Thank you. All the best.
Very helpful information.
But u have not mentioned after placing blood in N/10 HCl in sahlis tube wait for 10 minutes
And also
In no case we don't have to take the stirrer out, add distilled water but don't take the stirrer out as it will contaminate the solution.
Yes you are correct. Sorry for these
Thanks sir tomorrow is my hap practical exam
you are doing a great job . i have also performed this practical in our college svcp indore.
thankyou and i hope you will continue the great work...
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Thanks for explaining good explanation
Sir on the haemoglobin tube what does percentage (not Gram percentage ) denote? Today I performed this test. Gram percentage value was 9.6 and percentage value was 65%
This video was very good
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AweSome video ❤ thank u so much u clear my all confusionx
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Nice
Gudiya Kushwaha thank you
Thnxx sir for dmlt students May Allah bless you 🤗
Take 5 to 10 minats observe time than add drop by drop D. Water
swdesh sharma Exactly. We kept that for abt 8 mints but forgot to inform. Thanks
Awesome video sir..sir, but distilled water humne side wall se ku add kiya centr me se ku nhi??
Slowly jaye isilye
Kon kon meri tarah practical ke 5min pehle video dekhne aye😂😂
Very goodd sir....nice prctcl lecture
Thank you
Dude, who's gonna wait for 5 mins after adding 20 cu mm blood in dilution tube filled 0.1 with hcl for the formation of acid hematin.
Don't mind sir, remember he is a pharmacy wala.
Thanks sir it was very helpful for pharmacy students
Practical krte time dekhne Wale ........😂😅
Very well explained sir
Thank you
Humlog practical lab me byathkar ye vdo dekh rahe he
Very helpful, thank you sir 🙏
I obviously dnt knw better than u sir
But it’s not advisable to remove the stirrer everytime
Coz the blood loss occurs with the stirrer..!
P.S pls dnt get offended sir
Bt i just shared wt i knew :)
Zeel Kodia Your point is right. But we have already mixed the blood with DW so the blood volume is sufficiently maked up and it will affect the final result. Although your suggestion is highly acceptable and we are thankful for this.
Solution- Pharmacy Okay sir
Got that :)👍🏻
Ur VDO really helpful
Thank you
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Sab wahi kar rahe 😂😂
This is the most viewed video of the channel ..
shouldn't we wait for 8-10 minutes after adding blood in hcl ?
Yes we should wait for few mints.
yes correct...!!!!
Yes
Write
Something 10 minutes
Good morning sir ❤❤❤
tyx sir
DNYANESHWAR KADAM welcome sir
Exam will start from 12.30pm and I seen the video 8.20am .. direct going to lab first time perfoming the pratical without any experience confidence
@@krishnamonidas2345 kaisa hua tha practical ??
2.3 hours baad practical h😢
mera 20 min baad
@@liasiid 30 mins🙂
15 min
Thanks sir. today is my exam of HAP
All the best
You just gained a new subscriber. Thanks a bunch sir
Some laboratories multiply the meter reading with 8 and 9 to get the result in decimals
i,e:-
Meter reading.....13
13×8= 10.4 gms%
I still have a doubt
thnx sir tomorrow is my physiology exm u save me🙂
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Konsa course hai apka
DMLT wale like karo
thanku sir next week mera human phusiology ka practicle exam aa it is help full...
Sonpreet Singh Pleasure is our. All the best.
2 gante bad practical h 😢
Thank you sir
My pleasure
Waching this video just before 1hour of viva 😅😅and never done this practical in lab.
#backbencher 😂😂
Maine bhi test kiya hi sir thank you sir 🙏🙏❤️❤️
While mixing never take the glass rod out 😐😐....
Great 🙏🙏🔥
Thank you sir it really helped for my exam 🙏
Great. Thank you so much for your feedback
SUKRIYA 🤗❤️
Mashallah thats good explanation Thank you
Bohot acha hai sir 😊
Thank you so much
Thx.....yaar ... it's very helpful for mbbs student..... please make more videos
raj bhagora thank you. We have uploaded many practical videos and we will try to upload more videos