Gel electrophoresis | Chemical processes | MCAT | Khan Academy
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- Опубликовано: 16 сен 2013
- Learn how gel electrophoresis separates DNA and protein fragments based on size and why one would use agarose gel electrophoresis versus SDS-PAGE. By Angela Guerrero.. Created by Angela Guerrero.
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What happened to your voice khan?
and ur handwriting...XD....its elegant........
Yeah, his voice is fried...
I'm the 200th like
@@jervon3739 🏆🏅
I'm the 204th like. 😜
omg this is sooo simple and easy.....i really hate my class teacher
aw
yeah same
Me too
Me toooo :D
Lol! You just said what I wanted to.
Your voice made me come back to this video for the third time😁
I'm doing this stuff in gen biol lab and I have no idea what my professor is lecturing about ? After watching this 7 min video, I understand the concept completely. Great job
Her voice reminds me of "this one time, at band camp"...
say my name bitch
Wrong.
Yea thats true
Thank you for the great visual. This seems so much clearer now!
this is just great. It helped me a lot to learn this stuff. thanx
Nice Video! A note is that since as the DNA gets larger, more phosphates group are found on the structure, and thus the electric force is proportionately stronger, what causes different fragments to separate is a gel that allows smaller DNA fragments to pass easily whilst larger fragments much more slowly (similar to size exclusion chromatography where beads are used).
This video is really good. I've been struggling to understand the purpose of electrophoresis for my exam. It's actually a really interesting technique when it's described in this video! My textbook and lecture notes are so dull in comparison :)
One of the best explanation videos I have ever seen. Short, well depicted and easy to understand
Thank you so much for making this video, it was very helpful!
This was great! thankyou so much!!
Simple, good and easy to understand. Well done. Thanks for the video!
This is a great description of one of the earliest methods of separating DNA. It is amazing how far DNA testing has come from the days of gel electrophoresis.
The way you broke this down in very simple terms and easy makes me want to cry. Thank you 🥺❤️
Thank U guys so much I've really enjoy it and learnt how to do it ..... Perfect
and thank U Angela Guerrero.U were so good at it " BIG high 5 "
Thank you so much. I have no background on molecular biology or genetics but this made it easy to understand.
Thank you for the teaching of gel electrophoresis.
Bless you.
Great vid! Very clear
Your voice is soooooooooooooooo COMFORTING
Thank you to the lady that took her time to explain this concept , please ignore Ignorance from some comments, again Thank you, well done.
AMAZING!!!!!!!!!!
Amazing explanation, thank you.
Her voice is magnificent!!!
The polarity stated in the video is a little confusing since we tend to think of cations being associated with cathodes, and thus it would be (+) positive. As someone commented before: The cathode is where reduction takes place. The anode is where oxidation takes place.
However, the polarity of the cathode, or anode, can be + or - depending on the type of device. In a galvanic cell the cathode would be (+) positive. In an electrolytic cell the cathode would be (-) negative. if your prior reference had you thinking that the cathode is (+) positive, you aren't necessarily wrong.
+Brian Hanrahan I had to bust out my chemistry book because people were saying that she has the charges wrong, but she indeed has them correct.
Cathodes attracts cations which are positive charge. Just think of this: what charge would you think attracts positive cations? Negative. So cathodes are negatively charged... For isoelectric focusing, cathodes are usually basic because of negatively charged OH groups which again important for attracting cations. And anodes on the other hand are just the opposite.
جميل جدا وفهمت بالشرح ده اوووي
شكرا جدا 😊
Perfect.. I mean that deserves one
Thanks a lot!!!
Muchas gracias por su explicacion.👍
Enlightening , ty
Such a good video, made things really clear and fun to learn! Nice voice to listen to too - thanks so much! :D
Ure like a superstar thank U so much for this illustration "Big heart Emoji"
A very useful video!
Well done girl 👏👏👏👏👏! You are the best , big thanks 🙋🙋😙✋
Brilliant explanation!
explained very well. thank u
Hey beautiful tutorial.
How did you create the video? Love the drawings you made!
great video. thanks
Very well explained thank u so much
Thank You.
Mi English no good, nor nooor, but mi understand this very well mi friend :) ........seriously, appreciated! lol
Nice lecture, please I want to know the parts of the electrophoresis and their uses.
great work thanks
Omg I love your voice!! And your handwriting, so so pretty. I hope you decide to make more videos ❤❤
Thank you so much
hmm. very informative 👍
Very informative video !
Great video!
Thank you 🙏
I love loading samples onto gels
Simple and understandable
woww...it looks so amazing dear. Thank you so much for sharing. Stay safe and good luck :)
Thank youu
Thank you
thank you . I like BioM
Thanks
everyone on a a PC or laptop LISTEN
Make the video Full Screen
On your Numpad on the Right of your keyboard spam either 9 or 3
Make some SICK BEATZ
2 and 9 as well as 2 and 3..... I should be studying X(
Some of these comments are so rude...ya all ain't grateful enough this video just simplified so much
You are amazing
The video was useful
Agarose GEL also called "Native Gel Electrophoresis"
cause this was the first method, they usually call it NATIVE
This hardly explained anything. Left out entirely the mechanism of action. No mention on the charge differentiation involved in electrophoresis.
very nice
Umm... I'm using the Amgen lab and my "R+" band is over 10k basepairs when I compare it to the DNA ladder. Idk what to write, so I just wrote >10k bp to avoid incorrectness cuz I can't like predict whatever is over 10k.
She forgot to mention the use of EDTA and a UV light source to visualize the nucleic acid bands. The protein's disulfide bonds must be methylated in order to keep the proteins linearized.
Or maybe she actively chose not to include that topic. Hmmmm.
If you're doing Western Blotting, do you measure from the top or the middle of the bands (or the bottom)? For example, I have a thick band and am comparing it against molecular weight markers, but not sure where on the band to measure from.
I suggest measuring the bands from the center of the band which represents the greatest concentration of nucleic acid fragments. However, you may want to consult your advisor or laboratory instructor, or email the company which sells the nucleic acid size ladder and ask them about how their ladder is to be used with the particular nucleic acids you are working with.
Shukran(thanks), you just save my 10mks from a test quiz.
There is both increase in mass and charge how they get separated as the ratio of charge per mass is constant plz explain.
PAGE is for small DNA & Protein
But SDS-PAGE, if I am correct only for protein
I'm suprised that noone caught the anode and cathode charges.
Anthony Verdun Jr This is 3 years old but I’m writing this for modern viewers.. Anyways she god the charges correct, just the colors were switched.
Soo where to get that agarose or the other substance?
First time I see a capital "G" cursive like that in my life...
It's probably reaching heaven.
Been doing SDS-PAGE for 22 years.
small dna fragments move faster because of the voids in agrose gel are presents .. so the small dna fragments can passs the voids faster than bigger takes time to travees through voidss
nice handwriting :)
How do u cut it out of the gel
I don't know why you didn't discuss restriction enzymes and their function in gel electrophoresis. Very important part that was just left out...
I agree
Can agar be used instead?
Agarose might be used for DNA fragments of 50 bps- 500 bps, but in reality it is used for for those which are longer than 500 bps while polyacrylamide is used for those less than 500 bps. and is SDS used for DNA? It is not specifically mentioned in the video, but it seems it is implied. It is not necessary to negatively charge DNA( as it is already negatively charged) and that is what SDS does. Even if it is necessary to denature the DNA, Urea is used.
its quinn from zoey 101
Lol your right hahaha
what's on his right?
Lmao trueee
Since you can do DNA or proteins on SDS-PAGE, does this mean that proteins are somehow smaller than DNA since agarose can only do DNA? I always thought DNA was < proteins but guess not
Wow someone writing in cursive, I thought i was alone.
Better than the guy who kept stumbling on words ummm ahhhh uhhh ! Very informative video.
thankzzzzzzzzzzzzzzzzzzzzz
She sounds like Dora the Explorer LOL
swiper, no swiping!
swiper, no swiping!
swiper, no swiping!
Not at all
i thought cathodes were positive and anodes were negative?
cellulose acetate can also be a medium
it made it even more difficult
*based on their size and electrical charge
Shouldn't it be the other way around for the electricity conduction at the beginning of the video? The anode is the reduced conductor which means a negative charge, while the ca+hode is the oxidized conductor that results in a positive charge.
Actually she's right
I have a question "Will the DNA get absorb so that it may shown or it will show automatically"
Yeheueee
Who the fuck hired Vincent Adultman to voice this informational MCAT prep video?
I watched this 3 times still cant figure out what those 3 samples represent? I mean are they the same only mixed with different dyes? Or they are 3 different samples?
Mohammad Aghdaei pink is the ladder, yellow and green are unknown samples
Hey guys
Kinda reminds me of chromotography
Del
isnt anode the negative and cathode postitive
NO cathode is the one that attracts cations (which means it is itself negative) and anode attracts anions ( which means it is positively charged)...,
MilkyWay456 oh yea its an electrolytic cell thats why, i was thinking a galvanic cell
Yes i agree, she sounds like dora.......lol
sal's voice is better