Practical- Gene expression analysis through quantitative Real-Time PCR

[@talalalothman3538]

Thanks very much Dr. Ranjeet Ranjan & Dr. Suneha Goswami for this amazing session. I guess in 58:00 when adding primers, there is a mistake you added 0.8 uL of each primer to each of the 4 tubes of the master mix , it should be 0.4 uL of each primer to each tube. Once again thanks for sharing this session.

[@kalpanarautela320]

Hello there,
can you please help me with this ... How many treatments we can use at a time to get good results... Means to reduce background notice n for clear graphs etc ...
Thank you

[@nandiniga391]

Very nicely explained
Thank you sir

[@sanjayadhikary2608]

Thank you sir for nice informative video. Sometimes there is p value with FC what does this p value means. Is it the statistical difference between negative control vs treatment?

[@kalpanarautela320]

Hello sir,
I have a query related to experimental set up ...
Sir , can we take more than 2 treatments in one time for q PCR experiment using syBr green dye... If yes ...than how will we predict this in the graph as we have used the same dye in all the treatments..
Thank you 🙏

[@sumalatha1263]

Nice explanation, helped me a lot

[@noorpk]

Nicely Explained. Thank you

[@sumalatha1263]

Sir, can we calculate copy number through qpcr?

[@learningtape]

Thank You Sir. You resolved my worries

[@Tv7_telugu]

Amazing sir

[@somnathdamale3330]

Excellent lecture sir