Column Chromatography Separation (Sep-Pak C-18)

[@MikeT-hl7px]

Thank you for the video. I wonder how come to elute the red dye (less affinity to water comparing to the blue dye) with more polar mobile phase (ethanol 10%) and blue dye (high affinity to water) with less polar mobile phase (ethanol 90%).

[@jandresmusic]

What is the recommended flow rate? if the flow rate is too high, can the column break?

[@EarthC1_37]

What a column chromatography !

[@amalieshi293]

Thank you!

[@PrakashPulse4573]

Thank you very much

[@hamedkamal]

Please if its possible...
More details about chromatography

[@NCBioNetwork]

Anything in particular you are wanting?

[@channelnivens763]

@@NCBioNetwork would can cause the blue dye to elute first?

[@hamedkamal]

Jop opportunities

[@buxtonworld8765]

thank you very much