Column Chromatography Separation (Sep-Pak C-18)

Chromatography is a common technique to purify a pharma product. It’s how we break down a complex mixture into a single material. In this demonstration, we’ll use a mini chromatography column, the Sep-Pak C-18, to separate the red and blue dyes in grape soda.
This lab demo is just one of the many activities available in BioWork.
The BioWork certificate program will teach you the foundational skills you need to begin a career as a process technician for a biotechnology, pharmaceutical, or chemical manufacturing company. BioWork is offered at NC Community Colleges around the state. To find the one nearest you, visit ncbionetwork.org/biowork.
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[@amalieshi293]

Thank you!

[@PrakashPulse4573]

Thank you very much

[@buxtonworld8765]

thank you very much

[@EarthC1_37]

What a column chromatography !

[@MikeT-hl7px]

Thank you for the video. I wonder how come to elute the red dye (less affinity to water comparing to the blue dye) with more polar mobile phase (ethanol 10%) and blue dye (high affinity to water) with less polar mobile phase (ethanol 90%).

[@jandresmusic]

What is the recommended flow rate? if the flow rate is too high, can the column break?

[@hamedkamal]

Please if its possible...
More details about chromatography

[@NCBioNetwork]

Anything in particular you are wanting?

[@channelnivens763]

@@NCBioNetwork would can cause the blue dye to elute first?

[@hamedkamal]

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