LC Troubleshooting | Retention Time Shift | 5 most common causes to change in retention time in HPLC

[@alphacricketnews4546]

Sir please upload such type of video.
This video is really very helpful for the QC analyst who are perform analysis on HPLC. Thank you .....

[@EnglishExcel2208]

Thanks for ur appreciation

[@ganeshkapse4107]

Having quality in this channel , best

[@EnglishExcel2208]

Thank you so much....

[@mnmlibra6025]

1.HPLC column temperature
2. Leakage
3.sample overload
4. Column contamination, most common cause, wash column before starting analysis
5. Inadequate mixing and de gassing of mobile phase.. Sonicare Mp before analyzing

[@mnmlibra6025]

2.System leaks or flow rate instability

[@srinvasbhumi795]

Good explanation

[@EnglishExcel2208]

Thank you so much...

[@nkkumari7772]

Tq sir

[@rakhichahar5321]

Please make a video on instruments calibration, your video is very helpful.

[@EnglishExcel2208]

I will start calibration series very soon and thanks for your valuable comment........................................

[@rakhichahar5321]

@@EnglishExcel2208 Thanks sir

[@nkkumari9603]

Great information
Thank you

[@mangammollasrinivas6003]

Dear sir please do videos on HPLC RP method development how to learn method development I have Dought please do videos on method development

[@EnglishExcel2208]

Ok dear I will definitely make video on method development very soon.
Thanks for watching......

[@ankushkardileofficial579]

Not proper ph done. Which problem will come on hplc.

[@vijayparmar5455]

Superb 👍👍💯💯👌🙏

[@veenakumari6908]

Thank you

[@vijayparmar5455]

Thanks sir 🙏🙏