I have two doubts. 1. We have to add buffer to organic solvent while making mobile phase or organic to buffer 2.RT some times shifting about 1 min in one sequence what is the reason.
Mahesh Pds thanks for the nice questions. Answer of first question is: While preparing mobile phase the order of addition of solvents should be as given in individual specification. If specification says to mix organic phase and aqueous phase, it has to be ensured that organic phase is to be added to the aqueous phase and not vice versa.
I have a doubt can you guide me. RRF once arrived for a impurity 1.5. After that another person doing same the RRF is not same 1.5 . It is higher or lower got. How much percentage is allowable for RRF variation acceptance criteria anything in any guidelines
1.temperature fluctuation 2. improper degassing of MP.. air bubbles.. obstruct MP flow 3. leakage in hplc system.. if peak rt has increased due to leakage then flow rate will decrease 4. incorrect mp composition in reverse phase.. error of 1% in org solvent then rt shift of analyte can be 5-15%.. prepare mp gravimetrically 5. incorrect ph measurement of mp
Sir want more troubleshooting videos like that........Thank you for this information.
Good information
Good information
Keep continue 🙏
Thank you, I will
Good
Such a informative video...thanku...post every day so that we can learn more😊
Thanks Soumya Mishra …everyday little difficult… 🙂
Thanking you☺️
I have two doubts.
1. We have to add buffer to organic solvent while making mobile phase or organic to buffer
2.RT some times shifting about 1 min in one sequence what is the reason.
Mahesh Pds thanks for the nice questions.
Answer of first question is: While preparing mobile phase the order of addition of solvents should be as given in individual specification. If specification says to mix organic phase and aqueous phase, it has to be ensured that organic phase is to be added to the aqueous phase and not vice versa.
Nice explanation
Can u explain about DMF and types of DMF.
Saikrishna Lakkimsetty Thanks
Honestly speaking I don’t know much about DMF but I will learn about it make video in future..
Instead of these any other possibilities for rt fluxation
These are the major causes of RT fluctuation. Sometimes insufficient column saturation can also cause RT fluctuation.
I have a doubt can you guide me.
RRF once arrived for a impurity 1.5.
After that another person doing same the RRF is not same 1.5 . It is higher or lower got. How much percentage is allowable for RRF variation acceptance criteria anything in any guidelines
No variation accepted in RRF values… whatever given in your standard Method same you have to apply during processing.
Can you Say the guidelines number for this RRF variation not accepted
How much is the acceptable RT variation limit
10%
1.temperature fluctuation
2. improper degassing of MP.. air bubbles.. obstruct MP flow
3. leakage in hplc system.. if peak rt has increased due to leakage then flow rate will decrease
4. incorrect mp composition
in reverse phase.. error of 1% in org solvent then rt shift of analyte can be 5-15%.. prepare mp gravimetrically
5. incorrect ph measurement of mp
Useful video but not fresh all are old only