MOREL CULTURE BREEDING, Spores to Agar
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- Опубликовано: 5 окт 2024
- Collecting spores from wild picked or store-bought mushrooms is a fun and inexpensive way to get a culture started if you know how to do it. In this video I explain everything including proper spore printing techniques and spore germination on agar. Here I'm working with Morchella americana, but this technique applies to all mushrooms. It's important to remember that your spores won't be sterile, so once they germinate on your agar, it's best to sector off some mycelium quickly to another clean plate before contaminants can set in.
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That is exactly the mushroom I've been thinking about lately. Please tell us you will show us how to fruit in bag culture! Great video.
If I can figure out how to fruit them in bags, I can definitely quit my day job 😁 I'm finding it very challenging just to get them to fruit outdoors.
Thank you for sharing your journey so far! I’m excited to see where the grow goes next 💙🍄💙
I got my spore prints from my big yellow morels this year, by using a cheap disposable cake pan. I just set a sheet of foil in the bottom, put the mushroom on, added a drop of water to the top of the mushroom, and put the plastic vented lid on top. 24 hours later I had good prints from the larger, more mature mushrooms.
If you want to transfer spores make a metal wire loop, flame sterilize it, cool it in agar then just once touch the sporeprint and streak over the agar.
That way you will get less contamination than if you scrape it down as you did where all the contaminant have a better chance to drop as well.
Hey RM! Mark from Charlotte, great video as always! So are you planning on growing out those morels? I hear it’s very difficult.
Sorry, don’t bother answering…I read further down in the comments and you already answered a similar question😁
Yeah I'm making spawn now and doing some fall surface planting in a bed I made. I isolated four strains from these spore germinations and one of them (Strain 4) is very aggressive and forms sclerotia very quickly, so I'm going with that one for my spawn.
How do you feel about using grain water for misting or flush rehydration?
Also could you possibly do a video on fermentation of casing or substrate? I feel like it would be really easy to just fill a tub with coco, ver, straw, whatever. And just submerge it for two weeks, then simply remove as needed. It should technically keep substrate on hand and at the ready once the time line has passed. And you could technically have hundreds of pounds of the stuff for use, that you could just pick from, could make a years worth of casing mix in a matter of 2 weeks.
Then when you need it, grab some hand fills, strain to proper hydration levels and go. I don't think you have to pasteurize it or anything, just use it quickly once it's been removed and re-exposed to oxygen. But once exposed doesn't it create a better environment anyway?
Interesting ideas. I think the grain soak water would absorb nutrients from the grain so it may lead to increased contam risk if you misted or soaked with it. Never know till you try though. As far as substrate submerged water fermentation, it may work in the short term, but eventually it would break down. I have soaked sawdust and wood shavings for a full year and they eventually turned to black mush. It would be a cool concept to test out.
@RenegadeMushrooms I was just curious, and of course it would break down eventually. But I'm not a scientist or anything, but I feel like coco could potentially last quite a while. It is capable of pratically crossing oceans and that can take a long time in a fairly demanding environment.
I'm honestly very very new, but I grabbed some reptile coco chips, then mixed with regular reptile coco, ver, and I added some liquid cal/mag, bovine peptone, gypsum, and some calcium hydroxide. I didn't measure anything because I wasn't really thinking about it. And I just started to let it sit. But I'm sure you can do it better. This is my first run.
I used the reptile coco because it is suppose to be cleaner than gardening coco. So I tried to start with the most sterile product from the start I guess.
Can you show more of the end of the petri dish full of mycelium. Thx
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Hey RM...thank you so much for making this video and all the others. I'm learning a ton! A couple questions about growing spores if you get time...
first, how long did it take to get some growth going?
Do you ever need to hydrate spores in water, or can you always go straight to agar?
And I've tried a few different species of mushrooms from fresh spores I've collected (lions mane, maitake, and chicken of the woods) on agar that I made myself (both PDA and LMEA) and none of them showed any growth, even after 2 or 3 weeks. Any suggestions on what kinds of things might be going wrong? If it's too broad don't worry about it...I'll keep trying stuff.
I'm not sure, but maybe a quick soak in distilled water would help if they are too dry. Mine were very fresh when I used them, only a few days after printing. I saw germination at about one week with these and my plates are LMEA.
@@RenegadeMushrooms thanks for the reply! What I was using was less than a day old each time. I'm wondering if I screwed up making the agar. Because I didn't even get any visible contamination. Really appreciate the info.
@@mikel646 I don't pour my own agar, not enough time, too many hobbies 😁 I buy mine pre-poured from Midwestgrowkits.com. They've worked well for me so far with my agar adventures. I've found them a good company to work with.
@@RenegadeMushrooms Well I got stuff growing on agar now. The lions mane started to take off right after I posted. It even started to fruit on agar. I'm really stoked. Now I have what I *think* is a lions mane LC going. Or it could just be a jar full of trichoderma tho...lol. I'm gonna start a small jar of rye with it and see what happens. I really should have just bought an LC syringe but I'm enjoying the process of learning. Gives me something to do until I can get back in the garden. Really appreciate your videos man. If I ever get oysters to grow in LC, I'll be trying your grow bag pasteurization technique
Hey Renegade, Mark from Charlotte again. I have a general question. I made several jars of your LC medium recipe to inject some LC into. But life happened and didn’t get to it. The jars only have an injection port, no fae port. They have been sitting at room temp for about 2 months and have no signs of contamination. Reckon they are still OK to use or should I toss them? Thanks for all you do!
If the liquid shows is nice and clear I would probably try it. I've stored them in the fridge before for a month or so before inoculation and they turned out fine.
can't wait to see what they look like colonized, someone brought me 2 morels & the plates dont look like anything i've seen in the past, they are brown, black & do have some white growth to them.
some have what look to be sclerotium, kind of similar to what you see in wild honey mushrooms, thicker rope strands of dark mycelia.
I'm still learning too, but they are strange and challenging compared to the other mushrooms I've cloned. I'm looking forward to seeing how these strains look on grain. So far I'm liking the spore method on agar more than tissue cloning, but that may change 😅
@@RenegadeMushrooms I cloned mine & they are messy to say the least.
I thought it was impossible to grow morels at home. maybe that is a myth?
Quite a few people have had success with outdoor grows at home so that's what I'm trying.
So when you “clean up” the agar are you just picking an aggressive spore that’s expanded on the original dish and transferring it to a new agar dish? Thanks in advance
Exactly, so I'm selecting vigorous looking areas of mycelium on the original plates where the spores germinated and transferring those wedges to new plates. You can then watch those new plates for different behaviors. I transferred four wedges from the original plates and they all behaved a little differently, with strain 4 being the most aggressive.
@@RenegadeMushrooms thanks so much!