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This page will give you a good idea about transformation trouble shooting www.thermofisher.com/in/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-troubleshooting-guide.html?open=transformants#transformants
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Let's say 10 plasmids are there in the solution so ideally 10 should go inside ( assuming transformation efficiency is 100% ) . But in reality it's near 60-70% so 7 would make it and 3 would not . In reality we use plasmids in nanomol concentration. Now you can actually calculate the number of plasmid used for transformation.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
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very wonderful explanation, thank you so much Sir!
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this video is better than anything i've seen during my time at an american university, thank you
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In this technique is based on structure analysis or functional analysis of recombinant bacteria
Thank you Sir, it's very informative
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after doing all the process u mentioned, what if the colonies doesnt grow, any troubleshoot can be done? please tell all the possibilities
This page will give you a good idea about transformation trouble shooting www.thermofisher.com/in/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-troubleshooting-guide.html?open=transformants#transformants
amazing video
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Thanks! I was wondering if I could get the powerpoint for this?
Sure
Great information, Thank you sir
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What treatments did not produce transformed bacteria?
If you just put the dna and bacteria in a tube and don't give headstock then transformation won't happen
Best vid ever!!
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thank you
Please share my channel link with your friends and help me to reach big audiance
Does only 1 plasmid enter the E.coli cell? Or do many of the same plasmid enter the cell?
Many many plasmids
@@animatedbiologywitharpan If for example 8 plasmids are outside of the cell during incubation, do 8, 4, 3 etc plasmids go in? Or only 1?
Let's say 10 plasmids are there in the solution so ideally 10 should go inside ( assuming transformation efficiency is 100% ) . But in reality it's near 60-70% so 7 would make it and 3 would not . In reality we use plasmids in nanomol concentration. Now you can actually calculate the number of plasmid used for transformation.
Great explanation!
Please share among friends and help me to reach big audience
@@animatedbiologywitharpan I'll be sharing this video with other students in my biotechnology program!
@@mightiestmouse8833 I also study biotechnology! High five 🙌
Awsome explanation. would you like to share the Powerpoint?
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
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I am so sory but the powerpoint is chargeable and not available for free
You can buy this powerpoint by paying me rs 100 via super thanks
Thank you 👍
Please share my channel link with your friends and help me to reach big audiance . namaste 🙏
Sir please tell me, how to prepare plate with antibiotic?
Prepare agar plates. Dissolve antibiotic in water. Mix into cooled agar. Pour, solidify. Seal. Store airtight. Maintain sterility.
@@animatedbiologywitharpan Thanks sir
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What is the role of time in experiment? ex) 2min, 30min.... I want to know exactly......
2:50 for 2 min
4:00 for 30 min
The explanations are give at those time points
thank you!
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thank you so much
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What is the role of putting bacteria initially on ice.
Just to give it a heat shock
I am saving my ass before exam 😂❤😂
i am the ass saver. please share with friends and save their ass.
Thanks alot
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THANK U!!
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There was this mcq that confused me i.e the transformation efficiency increases if plasmid size increases upto ____ Bp
What were the options?
UpTo 15kb ..after that efficiency decreases
thanks sir
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Thanks
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@@animatedbiologywitharpan ı will :)
Thank you!!!!!!!!!!!1
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this is exactly how i do in my lab
😇
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sure@@animatedbiologywitharpan
Thank you sir
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what is the temperature at which bacteria can takes up the plasmid?
It's a heat shock ,it should be kept at 4C for 5 minute then 42C for 90sec and then 5 minutes in 4C .this shock would allow the entry
tested and proven ( TRANSFORMATION PATTERN ) Ingenuity Mags Process
👍👍
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Sorry but drop the accent - it is irritating
Your channel will grow
Thanks for your suggestion, it's my inherent accent