Thank you for the upload. Very helpful and thorough overview! However, I strongly feel that isoclonic controlling should be tested by the manufacturer, as this can be considered target validation, no? Unless, the purpose would be to check non-specific binding of the attached fluorophore to your cell population(s)...
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4:45 just so I understand this... after a 15-minute incubation, the fc block shouldn't wash off the cells, assuming it is able to adhere? Right?
Thank you for the upload. Very helpful and thorough overview! However, I strongly feel that isoclonic controlling should be tested by the manufacturer, as this can be considered target validation, no? Unless, the purpose would be to check non-specific binding of the attached fluorophore to your cell population(s)...