Viscous oil is treated similarly to solids. Use “wet loading” if it is soluble in a solvent that is of equal polarity to your initial running condition. Use “dry loading” if it is soluble in a solvent that is more polar.
There is a good paper by W. Clark Still in JOC (Vol 43, No 14, 1978, pg 2923) which shows a table of typical fraction sizes with different size columns, volume of eluant, and sample loading size.
In our lab, we usually stick to fractions about 1/10th of the column volume. For example, if you use 25g of silica gel you should collect fractions of about 3 mL. This can be adjusted depending on your separation as well. Larger fractions can be taken for very good separations and smaller fractions for poor separations. This can help limit mixed fractions.
Thanks to all the team members for providing the wonderful content I hope you will make many more videos which helps many students, research scholars & others to get to know the basic understanding of such techniques & methods
happy new year and Thank you so much for this video. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here? in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use? I mean is there ratio from silica gel to sample to use in column, please tell me
Awesome video! super informative and very digestable. Also, I really dig the music. Any idea what the name of the track is? The song name isn't cited in the credits; only the composer is.
a piece of filter paper covering the porous glass filter plate, can facilitate glass washing, or even make it feasible and glass-lossless maybe... sometime f.e. the precipitate from the quenching of LAH RM, that sometimes unfiltered through cotton wool(for example), fortunately does not block filter paper, allowing filtering. my favorite chose with THF+LAH RM - to quench with a minimum of plain water(only H2O) - until whitening - adding not faster than 0.5 ml of the next portion of water every 30 minutes. Then white suspension diluted with an equal volume of DCM, shake and leave to settle for 30 minutes-to form compact(only 5ml from 1gm of LAH) crumbly solids, that can be filtered by gravity through cheesecloth... results flowing slow only with some rare waxy-nature products(but not with bad weather influence), that also accompanied with same difficulties in tradition LAH workup procedure, but even in that slow-workup synthesis - you will not need a three-liter Schott funnel instead of a one-liter cloth filter in pressurized-filtering-jar
thank you. If I used wet packing of silica gel to column (slurry), here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
If I have solid sample containing components, can I use any solvent to dissolve it? Or I have to use only the same mobile phase as solvent to dissolved sample? Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot in advance
Where in the world would i buy the powder for the silica gel or search on ebay or amazon to get it?!?! Please help me, and would any university carry it at their chemistry stock room? Thanks so much for the vid!
Allway - chemistry rules at least the atomic world, I want president David Nutt (h t t p s : / / w w w.) ruclips.net/video/2Oc_GF65nbg/видео.html be protonic - be smart! :-))) even carbolic acids better than "neutral hydroxyl in society"
consuming a synthetic product is better than intake of unificated killing, you can even get rid of oxidation process - by reducing vinegar or acetaldehyde, but egregor's bad reputation defiles even something just marked by some individual sign of degradation
Conocí a colegas que purificaban en una bureta y corrían al Carls Jr por que están plenamente convencidos de que el sueño americano es posible para ellos.
i am workin on column shromatography.. can you help me in guiding that is there any way of setting some manual punp system to my column that will convert it from standard column chromatography to flash chromatography...
You use pressure to force the solvent mixture through the glass as opposed to relying on gravity alone to perform the run which would take a substantial amount of time in some cases
They deserve an oscar
💀💀💀
I was thinking the exact same thing! LMAO!
Such a cliff hanger too, did he get to pack her column at the end?
Really well done. Much better than any of the corporate RUclipss on the subject with a nice reference to the foundational publication on Flash.
I liked how you cited a real scientific article and not just a blog's post, like every other tutorial on the internet :P, thanks
5:25 Sick burn!
Jesus XD
Labs *this* clean only exist in movies =D
never partake crude
Viscous oil is treated similarly to solids. Use “wet loading” if it is soluble in a solvent that is of equal polarity to your initial running condition. Use “dry loading” if it is soluble in a solvent that is more polar.
There is a good paper by W. Clark Still in JOC (Vol 43, No 14, 1978, pg 2923) which shows a table of typical fraction sizes with different size columns, volume of eluant, and sample loading size.
Wow this is a great video! I'll probably have to watch it a few times to keep up with everything however!
In our lab, we usually stick to fractions about 1/10th of the column volume. For example, if you use 25g of silica gel you should collect fractions of about 3 mL. This can be adjusted depending on your separation as well. Larger fractions can be taken for very good separations and smaller fractions for poor separations. This can help limit mixed fractions.
This video is incredible. Excellent work!
And later, Nathan gave Kristy a column of his own.
You are so disgusting!
Thanks to all the team members for providing the wonderful content I hope you will make many more videos which helps many students, research scholars & others to get to know the basic understanding of such techniques & methods
Best flash column tutorial on youtube that Ive seen
The best thing in this video is how much Silca is needed . You guys did a fabulous job👌
Awesome video! My mentor suggested I watch it and it was super helpful!!!!
This helped me review a lot to get started with my research thanks!
Great video. Cheers from UC Berk.
happy new year and Thank you so much for this video. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me
Great Video, Thank you so much💖💖💖💖 I will use the dry loading next time, unfortunately, I did not know about it before.
Amazing video!
I would like to read the paper that it´s mention in the video, could you drop it here please.
Awesome video! super informative and very digestable. Also, I really dig the music. Any idea what the name of the track is? The song name isn't cited in the credits; only the composer is.
a piece of filter paper covering the porous glass filter plate, can facilitate glass washing, or even make it feasible and glass-lossless
maybe... sometime
f.e. the precipitate from the quenching of LAH RM, that sometimes unfiltered through cotton wool(for example), fortunately does not block filter paper, allowing filtering. my favorite chose with THF+LAH RM - to quench with a minimum of plain water(only H2O) - until whitening - adding not faster than 0.5 ml of the next portion of water every 30 minutes. Then white suspension diluted with an equal volume of DCM, shake and leave to settle for 30 minutes-to form compact(only 5ml from 1gm of LAH) crumbly solids, that can be filtered by gravity through cheesecloth...
results flowing slow only with some rare waxy-nature products(but not with bad weather influence), that also accompanied with same difficulties in tradition LAH workup procedure, but even in that slow-workup synthesis - you will not need a three-liter Schott funnel instead of a one-liter cloth filter in pressurized-filtering-jar
thank you. If I used wet packing of silica gel to column (slurry),
here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
If I have solid sample containing components,
can I use any solvent to dissolve it?
Or I have to use only the same mobile phase as solvent to dissolved sample?
Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot in advance
Very Beautiful Demo and Explanation!
This was excellent,thanks guys.
Where in the world would i buy the powder for the silica gel or search on ebay or amazon to get it?!?! Please help me, and would any university carry it at their chemistry stock room? Thanks so much for the vid!
sigmaaldrich.com
www.sigmaaldrich.com/catalog/search?term=silica+gel&interface=All&N=0&mode=match%20partialmax&lang=en®ion=US&focus=product
Oblivion dialogues in the Arcane University, Department of Alchemy
Educative yet entertaining...
how can you load very viscous oils? -is it the same technique as drying?
-by the way great video
I'm doing it tomorrow in the lab. Thanks from Freiburg, Germany :)
Nice Free advertisement fro Chemglass.
What is the correct volume you should collect for each fraction? How does it depend on the amount of silica or crude?
Do I add the sand also after solid loading?
Awesome video
Thank you people
4:43 wait what, everytime i do that, the silica is stick into the flask
Thanks! This helps a lot :)
All is well, except for TLC in a cup. There must be a Camera for TLC, saturated with eluent pairs.
Wait, Dr. Haim Wiezman, a chemist... is he planning to become the President of Israel one day? :-D
Allway - chemistry rules at least the atomic world, I want president David Nutt
(h t t p s : / / w w w.) ruclips.net/video/2Oc_GF65nbg/видео.html
be protonic - be smart! :-)))
even carbolic acids better than "neutral hydroxyl in society"
consuming a synthetic product is better than intake of unificated killing, you can even get rid of oxidation process - by reducing vinegar or acetaldehyde, but egregor's bad reputation defiles even something just marked by some individual sign of degradation
4:54 no that's not a solution, it's a heterogeneous mixture.
Kristy looks like she is having super hard time with her teeth.
great video
Thanks from U of Montreal!
Wow, another awesome video, Dr. Weizman. You know how to make learning Chemistry fun!
why isn't she blinking the the first two minutes
Conocí a colegas que purificaban en una bureta y corrían al Carls Jr por que están plenamente convencidos de que el sueño americano es posible para ellos.
With experiment both chemistry wahooooooo............
5:23 Jesus.. Christie is sassy...
Beautiful lady, beautiful chromatography, beautiful video!
i am workin on column shromatography.. can you help me in guiding that is there any way of setting some manual punp system to my column that will convert it from standard column chromatography to flash chromatography...
good job, thanks
So cool👏🏽👏🏽👏🏽
I would like that she takes care of my column.
Great🌺👍
Why is it called "flash" chromotography?
You use pressure to force the solvent mixture through the glass as opposed to relying on gravity alone to perform the run which would take a substantial amount of time in some cases
Nice video.
thanks
it's not a ''pie pet'' but a pipette
epic dialogue
Amé
Amazing video!
I would like to read the paper that it´s mention in the video, could you drop it here please.
Maybe if you read it yourself, you wouldn't need a link. Ever think of that?