Please ask any questions in the comments! This is a very easy and fun experiment to do, so I encourage you to try it yourself. LINK TO PAUL PYRO: / thepaulpyro
Ethanol bring out also other stuff, if you want a pure solution of chlorophyll inject some mL of LPG (lighter's gas) in the provette. Ethanol will become yellow (organic stuff) and LPG super green. Then separate and put some ethanol in the LPG that evaporates. I've tried the separation but never the UV light, great video :D
Hold up there Walter. LPG is LIQUID PROPANE GAS. "Lighters gas" is butane or in the case of a wick type lighter it's naptha. So tell us, what the fuck are you REALLY talking about. Thanks in advance.
And why and how do you "put some ethanol in the LPG that evaporates"? That makes, no sense at all. Kinda like the rest of your post. Basically word salad. The five likes in 6 years shows how few people are actually as mentally garbled as you. Wow. Just wow.
1. Be more respectful, I don't know you and you don't know me. 2. Be more respectful, I don't see why to insult. 3. Your comment deserves no response but I hope others can benefit from it. 4. LPG is not liquid propane gas but liquid petroleum gas, a mixture of propane and butane, the fuel of cars and lighters. If you have access to other solvents you can use those, but for a home experiment this is the most accessible, albeit very dangerous one. You must absolutely stay away from flames and heat sources. LPG is a gas at room temperature but when it is extracted from the can it remains liquid for a while because it cools down a lot. Then a liquid liquid separation is carried out to extract the chlorophyll and leave the other substances (mainly carotenoids) in ethanol. The two solvents are separated with a separating funnel. If solid chlorophyll is desired, it is allowed to evaporate, otherwise fresh ethanol is added. Here a video where they use this method ruclips.net/video/YXpfchNKqm0/видео.html. 5. Be more respectful to others and the world will be more respectful to you.
Gooferking, want to hear a sad story? We did a lab showing cell's surface area to volume ratio using potato cubes and Iodine. Teacher pulled out lab grade Iodine. We soaked the potatoes in it, and she then dumped it down the drain. Lab grade, very pure, Iodine. I cried.
Thumbed up this comment just to bump the individual of compromised intelligence who was the former "top comment", tying you with 5 upvotes. here, have my thumb.
Hello At 0:14 , what did u put into the test tube? We are not native English speakers so we couldnt hear what u said. Please reply us as soon as possible
The chlorophyll is fluorescing. This means that when the UV light hits chlorophyll, it excites electrons (causes the electrons to go to a higher energy state). When the electrons drop back to their ground state, they release light energy (at a wavelength that is longer than that of the light that excited them in the first place!).
By the time you boil the plant, the cells have expired which is required to extract the chlorophyll pigments. Chlorophyll is not a cell, but a pigment, in this case a protein structure. The alcohol solvent is required to keep the protein from completely denaturing. With a centrifuge, it would be possible to separate this solution into chlorophyll a and chlorophyll b. These may exhibit different properties under a black light, I have never tried this but am now willing to find out.
Hmm... did you heat it with a flame? Maybe it heated to fast, and the rapid heating caused a large build up in pressure, spewing ethanol everywhere. Anyways, are you and your lab safe?
+Gooferking Science Hi there, I think it would be useful if you mentioned the potentially explosive properties of chlorophyll extraction in your video description.
+Camroc37 Try salted-out rubbing alcohol (heat up the rubbing alcohol, mix with supersaturated salt solution, methanol and ethanol or isopropanol, depending on what you bought, will form as a layer at the top).
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Ethanol bring out also other stuff, if you want a pure solution of chlorophyll inject some mL of LPG (lighter's gas) in the provette. Ethanol will become yellow (organic stuff) and LPG super green. Then separate and put some ethanol in the LPG that evaporates.
I've tried the separation but never the UV light, great video :D
Hold up there Walter. LPG is LIQUID PROPANE GAS. "Lighters gas" is butane or in the case of a wick type lighter it's naptha. So tell us, what the fuck are you REALLY talking about. Thanks in advance.
And why and how do you "put some ethanol in the LPG that evaporates"? That makes, no sense at all. Kinda like the rest of your post. Basically word salad. The five likes in 6 years shows how few people are actually as mentally garbled as you. Wow. Just wow.
1. Be more respectful, I don't know you and you don't know me.
2. Be more respectful, I don't see why to insult.
3. Your comment deserves no response but I hope others can benefit from it.
4. LPG is not liquid propane gas but liquid petroleum gas, a mixture of propane and butane, the fuel of cars and lighters. If you have access to other solvents you can use those, but for a home experiment this is the most accessible, albeit very dangerous one. You must absolutely stay away from flames and heat sources. LPG is a gas at room temperature but when it is extracted from the can it remains liquid for a while because it cools down a lot. Then a liquid liquid separation is carried out to extract the chlorophyll and leave the other substances (mainly carotenoids) in ethanol. The two solvents are separated with a separating funnel. If solid chlorophyll is desired, it is allowed to evaporate, otherwise fresh ethanol is added. Here a video where they use this method ruclips.net/video/YXpfchNKqm0/видео.html.
5. Be more respectful to others and the world will be more respectful to you.
@@waltergiurlani9033 Thank you for being mature with the response/comment, as i really needed this response for a chemistry project im working on.
Really fun experiment
Is there any article related to your experiment
try checking the flourescence of blood, it might be green
Could you perhaps acheive a brighter fluorescence if you diluted the solution a bit?
Good question, and I am not sure of the answer. If you try it, let me know if it fluoresces brighter.
I want to try it but I don't have a UV light yet
Gooferking, want to hear a sad story? We did a lab showing cell's surface area to volume ratio using potato cubes and Iodine. Teacher pulled out lab grade Iodine. We soaked the potatoes in it, and she then dumped it down the drain. Lab grade, very pure, Iodine. I cried.
No, stop! I can't bear to hear it! That's so EVIL!!!!
Jesus christ people breaking beakers in my class nearly gives me a stroke, but that.... that's too much.
Thumbed up this comment just to bump the individual of compromised intelligence who was the former "top comment", tying you with 5 upvotes. here, have my thumb.
by doing this, does it only extract chlorophyll or other pigments too?
Can you use isopropyl alcohol instead of methanol?
he uses ethanol not methanol
yes
Do you kook the water? Or do you simply put the test tube into a very hot water batch?
How would you purify this chlorophyll ?
Hello
At 0:14 , what did u put into the test tube? We are not native English speakers so we couldnt hear what u said. Please reply us as soon as possible
Nguyen Ngoc I added ethanol to function as a solvent for the chlorophyll.
hello! what kind of UV light is that?
Your videos are great. How old are you?
What happens if you put the solution in sunlight? What kind of energy would it generate?
Same
So why does it show up red? Chlorophyll is like hemoglobin, is there any connection?
Gooferking Science Hmmm, interesting. Thanks for your response Gooferking and great science videos.
i would like to know as well
The chlorophyll is fluorescing. This means that when the UV light hits chlorophyll, it excites electrons (causes the electrons to go to a higher energy state). When the electrons drop back to their ground state, they release light energy (at a wavelength that is longer than that of the light that excited them in the first place!).
would you be able to burn the chlorophyll to get something like magnesium oxide?
Is it safe to drink
Are the chlorophyll cells still alive after the extraction process?
By the time you boil the plant, the cells have expired which is required to extract the chlorophyll pigments. Chlorophyll is not a cell, but a pigment, in this case a protein structure. The alcohol solvent is required to keep the protein from completely denaturing. With a centrifuge, it would be possible to separate this solution into chlorophyll a and chlorophyll b. These may exhibit different properties under a black light, I have never tried this but am now willing to find out.
So... Did you do it?
Nice, I remember being shown this ages ago in college, I have to try this again now :D Great vid ;)
Where do you get your ethanol?
You can also use Acetone.
blubb7711 is it any difference if I use acetone and hexane instead of any kind alcohol? will it effect the purify of the chlorophyll? Thanks
No mather how you extract it, the purify ist very low.
I remember doing this! Nice vid
Under uv should not keep hand
This was really interesting
did it really glow?
Yes. Pure chlorophyll glows almost crimson red under a black light.
AWESOME VIDEO! :D
Thank you!
Does it matter what leave u use
Not really. All leaves contain chlorophyll. You just might need to use more leaves depending on what plant leaves you use.
Preparing myself for the exam
Great vid for explaining
WOAH just did this and the ethanol exploded!!! It shot out the test tube!
Hmm... did you heat it with a flame? Maybe it heated to fast, and the rapid heating caused a large build up in pressure, spewing ethanol everywhere. Anyways, are you and your lab safe?
Gooferking Science Nope, just how you did. I think the leaves stopped the gas from escaping.
+Gooferking Science Hi there, I think it would be useful if you mentioned the potentially explosive properties of chlorophyll extraction in your video description.
Dipshit alert!
@@crucifyrobinhood waiting 6 years to reply so you don't get charged with insult on a minor
Cool
Will moonshine work in place of Ethanol? Lol
+Camroc37 Try salted-out rubbing alcohol (heat up the rubbing alcohol, mix with supersaturated salt solution, methanol and ethanol or isopropanol, depending on what you bought, will form as a layer at the top).
Do you think this experiment could work with broccoli if I use the head?
Most likely!