Hi, well, the setup of Focus Forming Assay is similar to the plaque assay. It is different in that the incubation is shorter (so it gets you results faster) and that the sites of virus infection are detected by immunostaining (so with fluorescent antibodies). This makes it particularly useful for viruses that do not lyse cells. The downside is that fluorescent antibodies can be expensive and that you need a microscope with the right illumination to see/count the sites of infection. Hope that answers your question!
You safed my day bro. Very good and fast explanation.
saved*
Thank you, sir. This was very helpful.
Thank you for the succinct explanation.
well helpful indeed. thank you
Thanks sir nice information
Does the number of infectious virons change after calculation
how do you fix sir?
PFA 10% or 4% is not discussed.
Thankyou sir
hi can you explain please how FFU works (focus forming units)
Hi, well, the setup of Focus Forming Assay is similar to the plaque assay. It is different in that the incubation is shorter (so it gets you results faster) and that the sites of virus infection are detected by immunostaining (so with fluorescent antibodies). This makes it particularly useful for viruses that do not lyse cells. The downside is that fluorescent antibodies can be expensive and that you need a microscope with the right illumination to see/count the sites of infection. Hope that answers your question!
ya It helps a lot, thank you so much :)
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Gay