You have not added any video since so long. Please upload more videos on techniques or on other topics as your teaching skills are excellent and made me understand each and every thing.
Teacher, what an awesome handwriting! Clear and easy explanation. Repeating the word ‘Student’ says too much about his deep motives to not only explain but also to make sure that students understand.
Thanks Hassan. Finally I found one student who can understand y I m repeating word's n time taking. This is how I teach. Please subscribe to our channel Hassan
Extremely good class. Explanation is too good. As he is repeating.. "students .. students"...no chance for any deviation..he is pulling students sooo nicely to his class.grt sir!!!
Sir your teaching methodology is so much nice and impressive. Your hand writing is also clear. When i watched your vedio my all concepts had been cleared and learned within minutes. Thanks alot sir.
Can FRET occur only from the S1->S0 transition in the donor where fluorescence is defined, or can it also occur in an S2->S1 transition as an impulse of heat for example? I suspect not because the wavelength of heat is very long and as such would not excite the acceptor, but please elaborate anyway.
That's a good question. I'm not an expert on the topic but here's what I think. First regarding heat,l'm sure you know that heat can be produced by any wavelength. Microwave can produce heat and so can Infra red, visible light and X rays. In fact any electromagnetic wave can produce heat regardless of the wavelength. As far as FRET is concerned apart from distance the other important factor is spectral overlap of donor and acceptor. What this means is that the wavelength at which donor emits should be the excitation wavelength of the acceptor. If you stick to these rules you can engineer FRET systems at any wavelength. In your example, as per my understanding, the donor is stuck in an S1 state. How would you confine it in that state? Its natural inclination would be to return to ground. But supposing that you can keep it there whatever wavelength it emits from S2 to S1 transition can certainly be use by any acceptor that gets excited at that emission.
You have not added any video since so long. Please upload more videos on techniques or on other topics as your teaching skills are excellent and made me understand each and every thing.
Teacher, what an awesome handwriting! Clear and easy explanation. Repeating the word ‘Student’ says too much about his deep motives to not only explain but also to make sure that students understand.
Thanks Hassan. Finally I found one student who can understand y I m repeating word's n time taking. This is how I teach. Please subscribe to our channel Hassan
Extremely good class. Explanation is too good. As he is repeating.. "students .. students"...no chance for any deviation..he is pulling students sooo nicely to his class.grt sir!!!
Sir your teaching methodology is so much nice and impressive. Your hand writing is also clear. When i watched your vedio my all concepts had been cleared and learned within minutes. Thanks alot sir.
Love ur explainations
Thanks dear
Bahut acha smhjaye hai sir aap thanku so much sir😊😊
Thanks
Excellent way to express every word and concept✌️👍🏼
Superb explanation 😊
Beautifully explained sir.
Very good explanation. Kindly make video on BRET as well
Explained very well and clear but could not say the applications of the technique. Make another video for the applications of FRET. Thanks
Well explained...
this is very nice video...
well explained sir!!
Sooo nice it soo helpful ....
Great job 👍
Thank you Sir
You have made it simple for us.
Thanks dear. Please subscribe to our channel
thanks a lot,,,, so clearly teaching this process.
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Nice explanation
Need more videos please make more videos !! And upload new ones with electronic boards or a tablet
amazing video, Thanks Sir
It's so clear! Useful!!
Thanks. Please subscribe to our channel
Great explanation sir
Very well explained
Such a simple and powerful class was this✨😊❤
So helpfully lect sir tanx
Thanks Tanveer. Please subscribe to our channel
Upload regular videos on remaining techniques as soon as possible please
Nice
Nice explanations thank you sir for give idea about this topic
What are the application of FRET in biology????
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❤❤❤
Sir what is relationship between fret and molecular orientation. Please sir
Thanks a lot sir
Thanks. Please subscribe to our channel
Can FRET occur only from the S1->S0 transition in the donor where fluorescence is defined, or can it also occur in an S2->S1 transition as an impulse of heat for example? I suspect not because the wavelength of heat is very long and as such would not excite the acceptor, but please elaborate anyway.
That's a good question. I'm not an expert on the topic but here's what I think. First regarding heat,l'm sure you know that heat can be produced by any wavelength. Microwave can produce heat and so can Infra red, visible light and X rays. In fact any electromagnetic wave can produce heat regardless of the wavelength.
As far as FRET is concerned apart from distance the other important factor is spectral overlap of donor and acceptor. What this means is that the wavelength at which donor emits should be the excitation wavelength of the acceptor.
If you stick to these rules you can engineer FRET systems at any wavelength.
In your example, as per my understanding, the donor is stuck in an S1 state. How would you confine it in that state? Its natural inclination would be to return to ground.
But supposing that you can keep it there whatever wavelength it emits from S2 to S1 transition can certainly be use by any acceptor that gets excited at that emission.
how FRET is useful in mutation detection ?
Thank u sir 🙏.
Thanks Naina. Good to know that my video help you to understand about the fluorescence. Please subscribe to our channel
Thanku sir
Thanks dear Harshita. Please subscribe to our channel
Great sir ..but give me blood cell counter reagents formula
Thanks Rajesh. Please subscribe to our channel
Just subscribed with pleasure.
Can you list a good reference ?
Thanks
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@@biovisionacademy3203 already did
It's a pleasure 😊
Student!
Hindi mein explain krege to or behtar rhega
Forster*