Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

An Open Access Protocol from JoVE - Cancer Research.
www.jove.com/vi...
The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.
To view more Cancer Research video protocols, visit www.jove.com/.