Thanks for recording this video. The production and use of reagents is not well covered by other RUclipsrs who often focus on the more "entertaining" aspects of chemistry.
I like the stories behind these reagents, because most of them had been used until the early 20th century. Nevertheless, I also like syntheses or reactions that are made to entertain, which is why I'm trying to keep it well mixed.
thanx a lot at first, I want to ask about Potassium sodium tartrate, Phenol, and Sodium sulfit can I use one of the three compounds or I must use the three
Hello, can you tell me more details about the color change of acetaldehyde? I recently did a experiment like this, and I don't know why the alpha-amylase only with DNS shows color like acetaldehyde in your video, it should be as yellow as starch + DNS, right? (And can you explain why the DNS color reagent is more like yellow-orange in tube, but the blank is like "pure" yellow?)
Thanks for video Sir..In few protocols, they are adding sodium potassium tartarate after boiling the test tubes in water bath.What can be the reason behind it? Do we need to add it again as it is already added during preparation of DNSA reagent..
I guess it might have to do with the sugar being oxidized, which causes the aldehyde group to turn into an acid. This decreases the pH in the reaction. Maybe it is added afterwards to act as a buffer.
The higher the amount of the reducing compound is, the more 3,5-DNS can be reduced, which leads to more of the red coloured 3-Amino-5-Nitrosalicylic acid. :)
I didn't add these compounds, in this case. There are lots of recipes you can find on the internet, which do contain the other compounds, but they're mostly added to store the solution for a longer period of time or to increase the sensitivity.
Would it be possible to do something similar but in the other way around? Like trying to find the amount of Glucose in a certain solution, compared to another, using 3,5-DNSA? Also, if we had for example apple juice from different varieties, and we diluted them into different solutions, would we still be able to do this experiment and find which apple contains the most glucose?
@@RaExpIn okay thx :). I was planning on doing a chemistry thing on what I just described, and i wanted to make sure it could work. Thank you for the great video :) and have a great day
Thanks for recording this video. The production and use of reagents is not well covered by other RUclipsrs who often focus on the more "entertaining" aspects of chemistry.
I like the stories behind these reagents, because most of them had been used until the early 20th century. Nevertheless, I also like syntheses or reactions that are made to entertain, which is why I'm trying to keep it well mixed.
Lovely bit of scientific procedure here. I might have to show this to my students.
Thanks a lot... it was highly appreciated evenmore during the lockdown where the students did not have any access to laboratories.
Hello! For this laboratory activity, can I ask what book did you use? Do you have know any laboratory manuals or books with this experiment?
Thank you sir
Sir can you please tell what will be the temperature in water bath while incubating it for 15 minutes
thanx a lot at first, I want to ask about Potassium sodium tartrate, Phenol, and Sodium sulfit can I use one of the three compounds or I must use the three
You're welcome! There are lots of articles adding one or several of these compounds.
Hello! How much dilution should be performed like how many mls of distilled water should be added to bring the absorbance to a readable range?
Great job sir💯
Hello, can you tell me more details about the color change of acetaldehyde? I recently did a experiment like this, and I don't know why the alpha-amylase only with DNS shows color like acetaldehyde in your video, it should be as yellow as starch + DNS, right? (And can you explain why the DNS color reagent is more like yellow-orange in tube, but the blank is like "pure" yellow?)
Thanks for video Sir..In few protocols, they are adding sodium potassium tartarate after boiling the test tubes in water bath.What can be the reason behind it? Do we need to add it again as it is already added during preparation of DNSA reagent..
I guess it might have to do with the sugar being oxidized, which causes the aldehyde group to turn into an acid. This decreases the pH in the reaction. Maybe it is added afterwards to act as a buffer.
Can starch and glycogen be determined by this method?
can you show me, source of this method?
Is there explanation on why the intensity increase as the concentration of glucose increase?
The higher the amount of the reducing compound is, the more 3,5-DNS can be reduced, which leads to more of the red coloured 3-Amino-5-Nitrosalicylic acid. :)
THANK YOU!
You're welcome!
Thank you very much.
Hi sir 🙋♀️ Was titrimetric or gravimetric method used in this experiment?
Colorimetric :)
How much Potassium sodium tartrate, Phenol, and Sodium sulfit were added in this reagent? Thank you
I didn't add these compounds, in this case. There are lots of recipes you can find on the internet, which do contain the other compounds, but they're mostly added to store the solution for a longer period of time or to increase the sensitivity.
permission to download this video for learning in class lectures
Great video, thx
Good video
Would it be possible to do something similar but in the other way around? Like trying to find the amount of Glucose in a certain solution, compared to another, using 3,5-DNSA? Also, if we had for example apple juice from different varieties, and we diluted them into different solutions, would we still be able to do this experiment and find which apple contains the most glucose?
This is exactly what colorimetry in chemistry is about. You can compare the solutions with an unknown one and find out its concentration.
@@RaExpIn okay thx :). I was planning on doing a chemistry thing on what I just described, and i wanted to make sure it could work. Thank you for the great video :) and have a great day
You're welcome! Thanks :)
nice.
Thank you!