I found this video and other tutorial videos about ELIZA very benificial ,Thanks youssef Farhat, looking forward for more videoes especially about instruments and washing systems
I have a question. So for one 96-well plate, you are using the same capture/detection antibodies in every single well? Like, at 1:34, you have Samples 1, 2, 3 listed. The only thing that differs between them is the source of the serum sample, right? They all are being subject to the same antibodies? And in wells A2 and A3, they are the exact same samples, but you are performing it in duplicate just for consistency?
After preparing your antigen in the coating buffer, do you coat all wells including those of the blank too or instead you add equal volume of PBS into the blank wells? If I am loading in triplicates, does that also apply to the blank wells?
Thanks so much for this!! Was just wondering, you had your Std Curve 1 and 2 in first and last row. Was just wondering where you had your QC1, QC1 and QC2, QC2 (i.e. 4 extra wells?) Or were they not required in this instance? i.e. would it not only be possible to analyse 38 samples in duplicate rather than 40 in duplicate?
Sorry - what do you mean by QC1 and QC2? Also, once you get good enough at ELISA that you feel confident in your consistency, you can just use 1 well/sample. I've done that in the recent past to save money and time with good results - but consistency will vary from person to person. It's best to have replicate measurements if possible when you just start out with a new technique.
I found this video and other tutorial videos about ELIZA very benificial ,Thanks youssef Farhat, looking forward for more videoes especially about instruments and washing systems
Well. I couldn't find 90-well Plate Layout. There was no "print-outs" on your website.
I have a question. So for one 96-well plate, you are using the same capture/detection antibodies in every single well? Like, at 1:34, you have Samples 1, 2, 3 listed. The only thing that differs between them is the source of the serum sample, right? They all are being subject to the same antibodies? And in wells A2 and A3, they are the exact same samples, but you are performing it in duplicate just for consistency?
Correct!
Thank you so much man, you are science hero
Thank you so much for such great video. Please keep make more. Best teaching ever :)
After preparing your antigen in the coating buffer, do you coat all wells including those of the blank too or instead you add equal volume of PBS into the blank wells? If I am loading in triplicates, does that also apply to the blank wells?
Before doing ELISA I have to check total protein concentration
Thanks so much for this!! Was just wondering, you had your Std Curve 1 and 2 in first and last row. Was just wondering where you had your QC1, QC1 and QC2, QC2 (i.e. 4 extra wells?) Or were they not required in this instance? i.e. would it not only be possible to analyse 38 samples in duplicate rather than 40 in duplicate?
Sorry - what do you mean by QC1 and QC2? Also, once you get good enough at ELISA that you feel confident in your consistency, you can just use 1 well/sample. I've done that in the recent past to save money and time with good results - but consistency will vary from person to person. It's best to have replicate measurements if possible when you just start out with a new technique.
Thank you
Thank you
THANK YOU SO MUCH!