ELISA Tutorial 2: Coating and Blocking the ELISA Plate

Here, we illustrate the process and concept behind the initial steps of a Sandwich ELISA: coating an ELISA plate with a capture antibody and then blocking the plate with BSA or a detergent-based Blocking Buffer.
Our ELISA tutorials are kindly sponsored by Calbiotech. Click here to see Calbiotech's ELISA kits: protocol-place.com/Calbiotech
This video and other protocols can be found at our website, the "Protocol Place" - protocol-place.com/
A full ELISA protocol can be found at protocol-place.com/assays/sand...
This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series:
ELISA Tutorial 1: Understand How an ELISA Works - • ELISA Tutorial 1: How ...
ELISA Tutorial 2: Coating and Blocking the ELISA Plate - • ELISA Tutorial 2: Coat...
ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - • ELISA Tutorial 3: Prep...
ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - • ELISA Tutorial 4: Fini...
ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - • ELISA Tutorial 5: Prep...
ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - • ELISA Tutorial 6: How ...
Competitive ELISA Tutorial 1: How a Competitive ELISA Works - • Competitive ELISA Tuto...
Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - • Competitive ELISA Tuto...
Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - • Competitive ELISA Tuto...
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Thanks for watching!
Youssef Farhat
Protocol Place: protocol-place.com
**Check out some of our other tutorials via the links below**
Competitive ELISA Tutorials - - - • Competitive ELISA Tuto...
ELISA Tutorials - - - • ELISA Tutorial 1: How ...
Gelatin Zymography Tutorials - - - • Gelatin Zymography Tut...

[@clarkvergilamorillo5720]

Very informative! I didn't know how they made the ELISA kits we use everyday in the lab, not until now. Thanks a lot!

[@ibtisaamsheekh1588]

This is a very good tutorial. You explain it in a simple but informative way. I'm a biomedical student and were going to make an ELISA experiment next week. I feel more ready for it now. Thanks :)

[@teubert2]

@Leonel Johan You and Milan Fisher are scamming people. I reported you.

[@shakthiyathamizhchelvan5989]

Thank you so much. You explained in simple and lucid way.

[@greencard1245]

Thank you Youssef!

[@yousseffarhat1]

Thanks so much!

[@shamil91531]

Working together for a better future.. Done (y)

[@annas9942]

How much do you dilute the capture antibody? What concentration do you use and how much you add?

[@zacharybice5539]

do you have to have recombinant protein for your target antibody to make the standards if you DIY an elisa like this?