Introduction to the Light Microscope

Correct way to use a light microscope.

[@gracewingkalappurackal4401]

I'm here because of my virtual lab in microbiology..2020! Living in the ever changing times of education and an ever changing world.

[@carab.1083]

Thank you so much for posting this helpful video. I am in an online / distance Anatomy and Physiology class due to the pandemic and am needing to learn about this virtually...and this has made all of the difference! 🙂🙏🏻

[@BiologyIsForEveryone]

Glad it was helpful!

[@headdown1]

I'm awaiting delivery of my first microscope, and this was a really helpful tutorial for a beginner. Thank you!

[@shakaylabowdre2768]

thanks! cause I was looking like an idiot in lab

[@ManfromNowhere233]

Initial set-up:
1. Plug & Switch on
2. Adjust Rheostat - 80% light intensity
3. Place slide on stage & center using
stage controls
4. Turn Coarse focus Knob to highest
5. Turn Condenser focus knob to highest
6. Turn substage Condenser or Iris
Diaphragm knob LEFT so that max
amount of Light is passing through.
Additional steps for Kohler Ilumination:
7. Use 10x Objective Lens first.
8. Adjust coarse focus knob to bring
specimen into focus.
9. Turn Field Diaphragm knob full LEFT
10. Adjust inter-pupillary distance &
ocular focus.
11. Use centering screws to bring circle
to the center of the field of view.
12. Adjustment to Condenser focus
knob - tiny hairs length.
13. Open Field Diaphragm by adjusting
knob - so that the illuminated circle
is just beyond the field ofview.
14. Remove one eye piece & adjust
Numerical Aperture by adjusting the
Condenser (Iris) Diaphragm knob to
0.25, 0.65, or 1.25 as shown below:
(10x = 0.25NA)
(40x = 0.65NA)
(100x = 1.25NA)
15. Use the Fine focus knob to fine tune.
-----------------------
16. Repeat steps 14 & 15 each
time ...when switching to higher
objectives. No need to fiddle with
Coarse focus knob at higher
magnifications. And re-adjust Rheostat
to comfortable setting for prolonged use.
Note to self & others
- With love, from Canada.

[@knightingalesaid]

Thanks so much! That was very helpful !

[@Khemani_RL]

This was a perfect explanation!

[@HaoSci]

Neat! A very thorough introduction.

[@saadhussainleghari4831]

Great video

[@leenalsader8476]

Thank you very much! It really helped! 😊

[@limhyun3320]

Really helpful! Thanks a lot.

[@ScienceME]

Thanks dr.Shannon Meadows
Could u help me?
i need information about 3D-Optical Microscope and its using ..

[@leobluesy]

Interesting video,,nicely done,

[@jamaicansistarobinson7587]

Perfect for me since I start Micro in 2hrs!

[@moqafatamoqafata7144]

Thank u for this video.. enjoyed it

[@MrTudian]

Very good!

[@salvatauren]

very nice vid, thank you

[@Alexander8345]

Nice job, thanks a lot :)

[@MelissaM96]

nice,ty really helpful

[@SSSsajithajeninBioChem]

really useful one to the beginners

[@1beinki]

very helpful, thanks

[@saralopez9514]

Thanks I like how you teach

[@jasonalexjones6089]

Thanks babe, now i know to adjust my microsope

[@nyawirawaithaka4993]

Thank you!

[@chiluka77]

really useful thank you

[@sweetoneloves6811]

well done.

[@dietpiediaries6287]

Alcohol as lens cleaner vs actual lens cleaner solutjon?
Which type lens cleaner solution
can you recommend?
Which oil immersion can you recommend?
Thanks.

[@skomura]

thanks!!

[@Jack_Tilstone]

thanks

[@AndroidIdentity]

*click*, noice.

[@Mrswamy32]

very nice

[@tumuhairweemmanuel2268]

It's actually very good to know

[@rustyangel9265]

Can you use glass for a slide

[@dhimandatta6213]

Lordly fine.....

[@adeyanjugbenga5581]

Very helpful

[@lyndonvillaruz3405]

thx

[@nimadithsanjutha8431]

super

[@jennymariejolin2530]

DONE WATCHING. BSN 1-G

[@jennymariejolin6349]

From Sir Kevz

[@africanelectron751]

A pawn shop near me has one of these for the equivalent of $150

[@Suzukisenpai]

What`s a high-class gear! Leica - is the real name like Zeiss, Nikon or Olympus! ... very expensive ((

[@mireneheartguillergan3803]

from Sir Kevz

[@eshalnadeem244]

did any one notice she talks like ellen

[@moqafatamoqafata7144]

Yes she does ..

[@nyawirawaithaka4993]

now that you mention it yes she does!

[@briannastewart5871]

Lol

[@Martinjazz16799119]

you are mess

[@furonebi3567]

thanks