Thank you for making such useful video, if it's possible then pls add text steps by step for better understanding. And one more thing if you have sop of this procedure then pls let me know 🙏
دكتور ..هل ممكن الافادة رسالتي مستخلصات نباتيه ونفحصلهم antibacterial activity لكن في تناقض بين نتائجي ونتاىج المختبر بسبب عدم ايجاد مذيب مناسب للمستخلصات
Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?
You can try filtering through 0.65 u membrane filter. And if you have enough amount of the extract you can autoclave part of the extract. This will sterilize and checks heat stability
Sir if you do this video with complete information and slowly then it's very helpful for all students please
True
I find this very helpful. this is basically what I'm doing for my current research but I found your video a bit late.
Hi
Can I get your research work i need some guidance
Thank you for making such useful video, if it's possible then pls add text steps by step for better understanding. And one more thing if you have sop of this procedure then pls let me know 🙏
شكراً 🙏🏻🌹❤️
「明確なメッセージ、明確な構造、理解しやすい、ありがとう」、
thanks
Good explain but try to slow down the video speed.
if Antimicrobial from plant source are be trained which college would be better according to you sir / mam please suggest me 🙏
Sir if a conc. Of oil is to be in units such as 10, 20 and 40 mg/ ml of eucalyptus oil then how much amount of oil is actually to be taken
Can I ask what is the difference of antimicrobial activity test and antagonistic activity test?
Sir which agar media did u use?
ماهي الخطوات العمليه لتجربه دواء جديد حتي اخد الشهادات المعتمدة
Which plant you used?
Sir what if we want to use blank antibiotic disc for the plant extract..what will be the procedure
What was positive and negative control?
دكتور ..هل ممكن الافادة رسالتي مستخلصات نباتيه ونفحصلهم antibacterial activity
لكن في تناقض بين نتائجي ونتاىج المختبر بسبب عدم ايجاد مذيب مناسب للمستخلصات
هل انت بخير استاد😢؟
Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?
You can try filtering through 0.65 u membrane filter. And if you have enough amount of the extract you can autoclave part of the extract. This will sterilize and checks heat stability
@@abdelraoufelmanama7966 Thanks a lot for your nice suggestion. Will be very grateful to you, thank you.
كيف يمكنني تفسير النتائج بعد قياس القطر ؟
Can you please give me some idea how to check antimicrobial activity of 2cm or 2mm fabric
Can anyone tell which Broth is used for bacteria used here ( S. Aureus and other one)..?
You ended up not passing any message with the video, so fast, no laid procedure
Do you have a paper that I could cite for the method???
195.189.210.17/bitstream/handle/20.500.12358/25391/Elmanama%2C%20Abdelraouf%20A._4.pdf?sequence=1&isAllowed=y
@@abdelraoufelmanama7966 thank you so much
Which agar is used?
Muller Hinton
very interesting topic - unfortunately presented in a very amateurish way. too bad
No details or proper description is given. The concept and understanding remains very unclear.
Bs
If you don’t talk through the video how do we understand what you’re doing. Are you teaching or it’s for the views 🤭
Rip 😂 university of gaza