Part 4: Sample Prep: Room Temperature Methods - G. Jensen

[@Desmond.22]

🎯 Key Takeaways for quick navigation:
Chemical fixation using aldehydes like glutaraldehyde or formaldehyde is a common method to preserve cell structure for electron microscopy.
Osmium is used to infiltrate cells, making their structure more rigid and better maintained.
Dehydration and embedding cells in resin, such as Epon, allows for sectioning and staining with heavy metals like uranyl acetate or lead citrate for contrast.
Thin sections are cut using an ultramicrotome, allowing for detailed imaging of cellular structures.
Traditional EM thin section techniques have provided crucial insights into cell biology, despite limitations in resolution for molecular details.
Metal shadowing techniques, involving deposition of metals like carbon and platinum, provide contrast for visualizing molecular structures.
Negative staining techniques, using solutions with metal ions like uranium acetate, highlight protein shapes by surrounding them with a contrasting film.
Both metal shadowing and negative staining offer high contrast images for electron microscopy, revealing structural details of biological molecules.
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[@leicalove9384]

I think it is cute that the proteins drawn by Prof. Jensen look like peanuts.

[@Gingnose]

It is DNA polymerase, not peanuts😡

[@gilleslejeune6823]

Thanks. But how can you prove (in which studies) that all these preparation steps don't change or break the 3D shape of what you want to see ?

[@brainstormingsharing1309]

👍👍👍👍👍

[@jongseokchoi4038]

18:40

[@peasant12345]

poor mouse