ImageJ Tutorial 4.1 - Count Germinated Seeds Using Color Thresholding

This is chapter 1 of the fourth tutorial in our lab's series of ImageJ Tutorials! In this tutorial, we will show how to count germinated seeds to calculate the percent seed germination.

[@brendaoliveiradosreis752]

OMG, this video save me on my project. I have been count one to one only with my eyes , and sure this way with ImageJ is better and most confiable

[@UFHortLab]

Thank you so much for the comment! Glad to know that you found this video useful.

[@nurhabibahipb6269]

Hi, I really love your channel. Very useful for me as an agricultural student. Please keep giving more explanatory videos about how to measure seed radicles with imageJ. It will really help me to learn. Thank you very much.

[@UFHortLab]

Thank you so much for your comment! We're so glad that you like our channel and you found our videos useful.
Sure, we'll post more tutorial videos soon!

[@Magicbubble888]

@@UFHortLab Thank you very much, I apreciate that.

[@laraferrari1829]

I tried and gives me a error "No particles were detected. The threshold (255- 225) may not be corret. What should i do?

[@sebaaaaaaaaaaaaaa]

Thanks

[@UFHortLab]

You’re very welcome🙂

[@brendaoliveiradosreis752]

Its possible to measure the lengh of radicals by this software too ?

[@UFHortLab]

Sure! The segmented line tool is perfect for radical length measurements. It can easily measure the length of a curved or irregularly shaped object.
1. Calibrate your image by “Set Scale”
2. Select the segmented line tool - right-click on the line tool icon and select “Segmented Line”
3. Create a segmented line by left-clicking repeatedly following the shape of the object. Double-click when finished.
4. Analyze>Measure or Ctrl+M to get the measurement result.
Let us know if you have additional questions!