Counts in ImageJ

This is an easy way to obtain counts for scientific images in the free program, Image J. You will learn computer aided image analysis so that you no longer manually count cells, particles, nuclei or any other biological structure in both color brightfield and fluorescence (including confocal). You will use the Find Maxima function in Image J to aid in quantitation. Find out how to correct for uneven illumination, split color channels, use the Median filter to your advantage, make an outline (Region of Interest: ROI), and threshold tool. Use a reference image for counts/area. Determine how and when to use median filter and, possibly, unsharp mask. Set measurements in Image J for your units of measurement, and get surface areas and counts. Learn to make macros that work for automating quantization for image sets. Learn from an expert in the field, Jerry Sedgewick.

[@2karatekit]

There is no other video that has helped me this much. Thanks

[@maria-mz6wf]

that saved my life, thank you so much

[@EmlibyBlosery]

amazing! Thank you sooo much for this video!!

[@zahraahmadi2234]

Hello I have a question
I want to count hair graft with imageJ
( i have numbers of grafts but when I use this app the results is not correct )
Would you please help me ?

[@squidddwardieee]

Thanks!

[@rabbitreallifeinjapan2194]

thank you much for you details explanation, I am having trouble counting EBs　（embyonic bodys） in ImageJ because the light and dark colors are present together and the larger EBs are dark. Is there any way you can count this kind of pattern?

[@ScientificImaging]

As long as the embryonic bodies are on a uniformly toned background, these bodies can be blurred with a median filter. They'll turn into blobs of a tone that is lighter or darker than background. Try Maxima again and you should be able to get counts.