For E. coli, the interaction between primase and helicase is quite transient (at the order of seconds). The P16 domain seems to be responsible for mediating the transient interaction. For some other bacteria, the primase:helicase complex is stable for >30 minutes. Perhaps P16 (and the surface charge distribution for both primase and helicase) is the answer to your question, or may be something else. Unfortunately, I am not super up-to-date with the literature on this matter so that is the best answer I can provide at the moment.
Thank you for your question. There is no clear atomic resolution data on the DnaC:DnaB complex. There are studies suggesting both sides of the story, that is DnaC:DnaB can exist in either 6:6 or 3:6 configuration. The issue at hand is more subtle because it appears that DnaC which normally doesn't exist as dimer may form a dimer when it interacts with DnaB, so the 6:6 configuration is perhaps a auxillary DnaC:DnaC binding event, which transitions the 3:6 complex into a 6:6 complex. Since, there is no clear side to take the win, I cannot comment on what is the absolute truth in this matter. But for the sake of understanding (unless you are a researcher in the field) I think it isn't super important as to what the stoichiometry of the complex is, since it is still a debate. Excellent question and thank you for pointing it out :)
The DnaG allows the ssDNA to pass through it, meaning it scans the ssDNA for CTG. But as soon as the CTG is found and makes contact with the primase, that ssDNA is stuck and scanning stops (i.e. ssDNA is not allowed to pass through anymore).
This is brilliant! Thank you for breaking it down so well
What a lecture sir
Please share the source..
Love your explanation btw❤
Superb!
can you please elaborate on how primase and helicase physically separate
For E. coli, the interaction between primase and helicase is quite transient (at the order of seconds). The P16 domain seems to be responsible for mediating the transient interaction. For some other bacteria, the primase:helicase complex is stable for >30 minutes. Perhaps P16 (and the surface charge distribution for both primase and helicase) is the answer to your question, or may be something else. Unfortunately, I am not super up-to-date with the literature on this matter so that is the best answer I can provide at the moment.
soo good
According to Lewis XII Hexamer of DnaB binds to 6 monomers of DnaC contrast to what you have mentioned, can you please clarify on this,
Thank you for your question. There is no clear atomic resolution data on the DnaC:DnaB complex. There are studies suggesting both sides of the story, that is DnaC:DnaB can exist in either 6:6 or 3:6 configuration. The issue at hand is more subtle because it appears that DnaC which normally doesn't exist as dimer may form a dimer when it interacts with DnaB, so the 6:6 configuration is perhaps a auxillary DnaC:DnaC binding event, which transitions the 3:6 complex into a 6:6 complex. Since, there is no clear side to take the win, I cannot comment on what is the absolute truth in this matter. But for the sake of understanding (unless you are a researcher in the field) I think it isn't super important as to what the stoichiometry of the complex is, since it is still a debate. Excellent question and thank you for pointing it out :)
sir at 13.36 it is not clear, what do you mean by "DNA is not allowed to pass"
The DnaG allows the ssDNA to pass through it, meaning it scans the ssDNA for CTG. But as soon as the CTG is found and makes contact with the primase, that ssDNA is stuck and scanning stops (i.e. ssDNA is not allowed to pass through anymore).
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