Agar vs Liquid Culture
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- Опубликовано: 27 авг 2024
- Discussion about the differences (Pros and Cons) of Agar vs Liquid Culture.
Which one is better for you?
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A lot of good stuff in this vid.
I have noticed that LC inoculates grain very fast. I only use 1 cc in 2 or 3 places in the grain. Only 2cc or 3cc total in a jar to keep it from getting too wet. After 1/3 to 1/2 of grain is colonized, I shake the jars to evenly distribute mycelium. A week later the grain is fully colonized. A few wedges of agar in a grain jar can take 2x as long to colonize. Thanks for the videos. I like how you talk about the fundamentals and also go into details of your personal experiments.
That sounds like perfect technique. My clumsy hands have trouble being precise withbthe syringe and may times I squirt too much LC 🙄. Agar is faster than LC for me, but I like to use a lot of it to outpafe any contamination. But I use both here and there.
Thanks for your comment!
I see that you have 4 filters per jar, isn't enough with one filter?
Ziploc Grain Bags colonize much faster than any jar... Before I discovered that, I started testing by adding more Fresh Air Exchange on my jars. I found that the more filters I added, the faster the Mycelium would colonize. I din ever use Grain Jars anymore because the Ziplocs are so much faster...
How come using a spore syringe is a bad idea for Liquid Culture?
Spore Syringes are a crap shoot. You never know what you're gonna get... (unreliable genetics)
I have a video explaining the Mushroom Reproduction Cycle and we want to Clone:
ruclips.net/video/M4cL0H4blH4/видео.html
It's a deep dive!
@@KitchenMycology Thanks for the swift reply, I'll check it out! Also subscribed.
Would using a spore syringe be okay If you can ensure your using the correct Spores, like I've made my own spore print from a mushroom I cultivated?
@@osiris0087 You can use a spore syringe, but it's better to use them to inoculate grain. Then Clone there best fruit from the best cluster - Clone tissue to Agar... Then multiply your Agar, and/or use that Mycelium you grew in the Agar to inoculate Liquid Culture.
The idea is to keep transferring mycelium A2A, which propagates the strongest mycelium, creating a Monoculture of strong genetics.
I go over this and more in the Full Course, but that video I just sent you gives you a lot of details to understand why it works. 😎👍🏼
I filled a couple of syringes with sterilized water and let it cool. I squirted one (10cc) in to an agar plate and scratched the heck out of the surface. I flame sterilized that and shot it in to an LC jar. It's full of itty bitty mycelium - and a rock. Its Karo and distilled water. PC for 30 minutes.
Perfect! That's exactly how I do it. 😎👍🏼
Nice Video. Are you using a proper Petri Dish or something alternative? I was looking to make 50-100 Petri dishes but looking for a cost-benefit or alternative solutions for Peri Dishes. Thanks
Thanks! I use these PP5 Containers from Amazon. I do a "No-Pour Method" - Pressure Cook them already poured. I have a video in my Patreon.
These are good because you can eeasily re-use them with the "No-Pour Method."
PP5 Containers:
a.co/d/1fnmUXC
Do these wont deform when pressure cooked?
@@Vellieveil_64 They don't deform. They're awesome.
@@Vellieveil_64 You can also buy empty Glass Petri Dishes and reuse them.
Used condiment cups 200 for 10 bucks