The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper. I hope it'll help :)
Fantastic video. Very detailed, has all the key information and it's broken down simply :) Thank you.
Glad it was helpful! 🙌
thanks very much....finally understanding it!!!!! and it isn´t that complicated as it looked during the class :D
With this piece of video, you just saved my Genetic Engineering exam. Good job!
thank you VERY MUCH for your hard work!
Thanks so much! Super helpful for my test! :D
amzing, very usefull for me and all people who speak english as the second language.
lol....."dis is not a harmburger trust me" really enjoyed dis video.....thank you very much Sir !!!!!
Great video, thank you so much for that, usually scientists have to learn all the tecnics that we must use for yourself, and this video helps a lot!
Thank you for uploading this video. It is very helpful to understand the concept.
U the man! i get every word through the video... keep doin what you doin..
Great video! very clear... I couldn't find the southern blot video. If you have the link, I'll be glad
thank you so much. it helpful for my seminar^^
Please, make a video about western blotting! Great video and thank you!
Clear, practical and well detailed! Thanks a lot!
Very easy to understand.
in pcr we used primers that bind to the specefic complimentary dna sequences. but how can a cdna bind to rna . i dont understand
this video was very helpful,thanks :)
Glad to read that! We love their images too! :)
What are the limits of detection of this experiment? Eg. sensitivity? What if you're trying to detect small siRNA or miRNA?
Thanks, it's very helpful and clear
Glad it was helpful! 😊
Very useful. Thank you very much for the guide
Loved it! Extremely well explained.
is it possible to use SDS-PAGE instead of formaldehyd when openining the hairpin-structure of the RNA? (time 9:10)
Thank you! :) I'm glad you enjoyed it!
wow! great tutorial
this video helped me soooo much! you used the correct terminology I was looking for. I appreciated the help!:)
Great stuff!
This was really good. Keep it up!
great piece of work..really appreciate....simple and up to mark...
Great vid. Thanks!
you saved me, thank you!
Does formaldehyde just act as an acceptor of hydrogen bonds between base pairs?
Can we profile multiple RNA with this rechnique or it is only once at a time? Thanks
Very helpful and clear! Thank you!
I believe the filter helps with the labeling treatment.
AMAIZING
You just saved my life!!
Good explanation.
Glad it was helpful!
Cool, though usually amino benzyloxymethyl filter paper is used instead of nitrocellulose due to its higher affinity to RNA :)
Muito bom o texto/desenhos e apresentação, fácil compreensão do ingles. Parabens!
Your video on southern blotting is offline. Can you please reupload it?
Thank you!
Excuses me !! This video explain about sample preparation of northern blot right ??
is there a video for western blotting that is this good?
Really helpful I love it !
Awesome!!!!!!
Great video! Thank you for the tutorial. God bless.
Loved it . Great video thanks
nice job
Which computer program is used to explain this technique?
Thinking about covering that topic soon! :)
Hi! great videos!
Where can I find the southern blot tutorial ?
Thanks!
a lot of your past videos, including the one about Southern blotting is no longer available, why?
very great presentation, I appreciate the sense of humor :) , correct me if I am wrong, should the cDNA be a complementary sequence?
You are welcome!! :)
You're welcome!
Does these filters/membranes has those labeled probes? Or what's the point with the filter?
how do you extract rna and not dna?
Genio!! GRACIAS!!!!
Nice vid...well polished too.
very clear!!! thanks!
where is your southern blotting video?
How would this work with siRNA?
This question is for anyone who is an expert with this stuff.
why is the reason of the salt solution? :)
The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper.
I hope it'll help :)
Thank you!
wait arent u from kenhub?
why we use paper towels ?
Is Roger from American Dad narrating this?
Yes. 👽