The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper. I hope it'll help :)
Fantastic video. Very detailed, has all the key information and it's broken down simply :) Thank you.
Glad it was helpful! 🙌
With this piece of video, you just saved my Genetic Engineering exam. Good job!
Thanks so much! Super helpful for my test! :D
thank you VERY MUCH for your hard work!
thanks very much....finally understanding it!!!!! and it isn´t that complicated as it looked during the class :D
Thank you for uploading this video. It is very helpful to understand the concept.
U the man! i get every word through the video... keep doin what you doin..
Great video, thank you so much for that, usually scientists have to learn all the tecnics that we must use for yourself, and this video helps a lot!
Great video! very clear... I couldn't find the southern blot video. If you have the link, I'll be glad
Clear, practical and well detailed! Thanks a lot!
Please, make a video about western blotting! Great video and thank you!
Loved it! Extremely well explained.
this video helped me soooo much! you used the correct terminology I was looking for. I appreciated the help!:)
lol....."dis is not a harmburger trust me" really enjoyed dis video.....thank you very much Sir !!!!!
Glad to read that! We love their images too! :)
This was really good. Keep it up!
Thank you! :) I'm glad you enjoyed it!
great piece of work..really appreciate....simple and up to mark...
amzing, very usefull for me and all people who speak english as the second language.
You just saved my life!!
Very helpful and clear! Thank you!
you saved me, thank you!
Great stuff!
Very useful. Thank you very much for the guide
thank you so much. it helpful for my seminar^^
wow! great tutorial
AMAIZING
this video was very helpful,thanks :)
Very easy to understand.
Your video on southern blotting is offline. Can you please reupload it?
is it possible to use SDS-PAGE instead of formaldehyd when openining the hairpin-structure of the RNA? (time 9:10)
Hi! great videos!
Where can I find the southern blot tutorial ?
Thanks!
I believe the filter helps with the labeling treatment.
Great vid. Thanks!
Really helpful I love it !
Thanks, it's very helpful and clear
Glad it was helpful! 😊
Loved it . Great video thanks
Great video! Thank you for the tutorial. God bless.
Does these filters/membranes has those labeled probes? Or what's the point with the filter?
Thinking about covering that topic soon! :)
very great presentation, I appreciate the sense of humor :) , correct me if I am wrong, should the cDNA be a complementary sequence?
You are welcome!! :)
Awesome!!!!!!
Good explanation.
Glad it was helpful!
What are the limits of detection of this experiment? Eg. sensitivity? What if you're trying to detect small siRNA or miRNA?
You're welcome!
Thank you!
Can we profile multiple RNA with this rechnique or it is only once at a time? Thanks
very clear!!! thanks!
Which computer program is used to explain this technique?
a lot of your past videos, including the one about Southern blotting is no longer available, why?
Nice vid...well polished too.
Does formaldehyde just act as an acceptor of hydrogen bonds between base pairs?
in pcr we used primers that bind to the specefic complimentary dna sequences. but how can a cdna bind to rna . i dont understand
is there a video for western blotting that is this good?
Excuses me !! This video explain about sample preparation of northern blot right ??
nice job
Muito bom o texto/desenhos e apresentação, fácil compreensão do ingles. Parabens!
Genio!! GRACIAS!!!!
Cool, though usually amino benzyloxymethyl filter paper is used instead of nitrocellulose due to its higher affinity to RNA :)
how do you extract rna and not dna?
where is your southern blotting video?
wait arent u from kenhub?
why is the reason of the salt solution? :)
The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper.
I hope it'll help :)
Thank you!
How would this work with siRNA?
This question is for anyone who is an expert with this stuff.
why we use paper towels ?
Is Roger from American Dad narrating this?
Yes. 👽