Culture Media (Part 1)- Animal Cell Culture
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- Опубликовано: 18 сен 2024
- What is culture media ? It is a nutrient preparation that contains essential nutrients required by cells for their growth.
Two types of culture media :
- Natural media
- Artificial media
Natural media consists of naturally occurring biological fluids such as plasma clots, biological fluids like amniotic fluid, insect hemolymph, serum, tissue extracts.
Artificial media are grouped as:
- Basal media
- Serum media
- Serum free media
- Protein free media
Basal media - also termed as chemically defined media as all the components are known.
Components of Basal Media includes:
- balanced salt solution : provides an environment that maintains the structural and physiological integrity, pH and osmolarity of cells in vitro
- buffering system : regulates pH. these are of two types, natural and chemical
- energy source : commonly used is glucose. other sugars like maltose, sucrose and fructose are also used
- amino acids : increase cell proliferation.
- proteins/peptides : albumin act as binding protein, aprotonin act as protective agent and fibronectin aids in attachment of cells to the substrate
- growth factors : act as signal and helps in cell proliferation and differentiation
- vitamins : act as coenzymes involved in cell metabolism. eg vitamin B complex
- trace elements : copper, zinc and selenium are elements needed in micronutrients. These are essential for biological processes
- antibiotics : prevents contamination
- phenol red : serves as a pH indicator
WATCH the next video for Information on SERUM MEDIA, SERUM FREE MEDIA and PROTEIN FREE MEDIA
अति उत्तम विख्यान धन्यवाद
Thank you!!
Nyc session ur way of teaching is mind-blowing wow supb mamb
Thank you so much!!
Useful ❤️
Thank you 🙏🙏
Ap ki viedo best u tube pr ap se acha koi bhi bio tech nhi padha sakta h mam
maam this was much much better than my college ACC teacher 🥰🥰🤗
Thanks a ton🙂
@@SCIENCEAMBIENT786 maam, could u please make such lectures for PTC{plant tissue culture} and immunology too. it would be a great help for us . im in biotech honors and such good informative and on point videos are really hard to find for our subjects .
For sure :) will soon upload on the above mentioned topics... thank you!!
@@SCIENCEAMBIENT786 thank you soo much ma'am, I'll share the videos to my frnds
Wow..brilliant explanation ma'am
Thank you 😊
With your knowledge, is it possible to create a media from certain plant cells for the process of cultured meat? Reason I ask is that a plant cell culture company mentioned supplying meat cell culture companies with plant material, and I can’t figure out how and why?
Mam plz recombinant of technology pr viedo la aaye plz mam
Sure dear.. 👍
Mam mujhe practical Mila hai.. prepration of animal cell culture media..to mam mai kaise use bnau?
Dear first decide which particular media you want to prepare. One way is to buy ready made media which has all the constituents already present. Simply bottle par mention hota hai ki kitni quantity you need to dissolve (for 1 Litre) in sterile water and then vaccum filter kar lo. Another way is ki aap media k components ko weigh kar lo and then unko mix kar lo sterile water me.. i hope this is helpful. In case u have any doubt to puch lena :)
Mam plz recombinant DNA technology pr video laye i need help u mam