Determine the pH of different soil sample 12th class bio practical

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  • Опубликовано: 18 сен 2024
  • Procedure
    (i) Weigh 10 g of the soil sample A. Add 50 mL of distilled water to soil
    sample to make a soil solution.
    (ii) Filter the soil solution through a filter paper and collect the filtrate in a
    beaker. Label it as soil solution -A.
    (iii) Take a clean dry porcelain cavity tile. Place 5 drops of soil solution A
    in three cavities of the tile
    (iv) To the 5 drops of soil solution present in one cavity add 5 drops of
    universal pH indicator solution. Note the colour developed and compare
    it with the colour chart given on the universal pH indicator
    solution bottle.
    (v) To the soil solution present in the second cavity, dip a small strip of broad
    range pH indicator paper (pH 2-11). Note the colour and compare with
    the colour chart given on the broad range indicator paper and get a rough
    estimate of pH of the sample solution.
    (vi) Choose a suitable narrow range pH indicator paper (for e.g. If the pH of
    soil is determined by you as 8.0, choose a narrow range 7.0 to 9.0) and
    dip a small strip of it in the soil solution present in the third cavity. Note
    the colour developed and determine the pH to the nearest possible value
    with the help of the colour chart.
    Repeat the same steps for determining the pH of sample B and C. Follow the
    same procedure for water samples collected from different sites.
    Discussion
    Based on the pH values obtained, categorise the samples into acidic, basic,
    neutral type.
    Record the plant species present in the site from which the samples are collected.
    Note for teachers: The colour developed should be noted against direct sun
    light. Also, sometimes the soil solution colour may interfere with the readings.
    Thus one has to be careful while making the observations.
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