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- Опубликовано: 4 окт 2024
- we have demonstrated here biofilm production by bacterial strains. Biofilm is a very important thing for bacterial colonization. This biofilm formation helps the bacteria to better colonize in the rhizosphere of plants. Ultimately resulting in better plant growth and nutrient supply.
Video Courtesy: Arnab Kundu, JRF, St. Xavier's College Kolkata.
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Online batch amra dada ... 2022 ei MSC pass korlam st xavier's microbiology te.. tomake prayi dekhe6ilam... but nijer start up er jonye CRA method niye kaj korte giye youtube korte first tomar video ti elo.... khub valo laglo...evabe aro upload korte thako new techniques. It will help us.
Thank you so much. Parle college a ese dekha koris khub valo lagbe amar.
Hm hm nischoi
Beautiful
Thank you so much
Thank you very much for the video.
Stupid question though, why didn't you mix the congo red powder into the medium before autoclave instead of autoclaving them seperately?
No questions are stupid. Actually if we mix the Congo Red solution before autoclaving then due to pH change at high temperature the dye may turn blueish. To avoid that error we mix Congo Red solution at low temperature (55°C). Thank you for reaching out.
Sir you put 8 gram from congored on 1liter of medium
But it is so much and I read in different researches 0.8 or less than 0.8 on liter medium
Can you understand me and give me guide??
I have provided reference of scientific articles in previous comments. Please check.
Please show result if u can to differentiate biofilm forming or not
Thank you so much for your question. This is a qualitative test for biofilm detection. In this video I have shown black coloured colony formation which is a mark of positive results.
What can we use for controls?
For control we can use any non-biofilm producing bacteria.
Bohot achcha...
Thank you sir 😊
Ek no. Bikramji. keep it up
Thank you man. Keep supporting us.
At last after streaking and incubating the bacterial colony grow. In the colony where is the biofilm and how to detect it.
In the last portion of the video you'll see a culture plate with black colonies. The black colonies are the indication of biofilm formation. When you invert the plate you can see that the sticky slurry like colony falling on the lead of the petriplate.
Sir, may I know the principle of Congo Red assay for biofilm production?
You may follow the article
doi.org/10.1016/S1413-8670(11)70197-0
Amazing 🔥😁
Thank you 😍
How many grams of the congo red stain do we need to measure in how many milliliters of water to get the concentrated aqueous solution of congo red??
Congo red concentration should be 8g/l. Here for 200ml of media I have taken 1.6g of Congo red and dissolved it in 50ml water. I have taken bhi agar for 200ml of media but dissolved it in 150ml distilled water. After autoclaving I have mixed 50ml of Congo red and 150ml of bhi agar to prepare the final media.
I have a doubt sir. Like you autoclaved Congo red separately, would I have to do the same if I’m using a different stain? (I’m using bromothymol blue) or I can autoclave it in the same flask?
You can add bromothymol blue and then can autoclave the media. But check pH of the media to get desired results.
You can also check 'himedia M1822' medium for further details.
@@thebioscientist can you tell me himedia no for the congo red agar, please?
Sir, if I make 100 ml of congo red agar medium how much composition distilled water i have to use for bhi medium and congo red? Thank you sir
60 ml for bhi and 40 ml for Congo red. Thank you for your queries. I was unavailable due to some unavoidable situations. Thank you so much.
from where did you get the amount of each component?
I have already posted the link of the paper in comments section. Please check.
Sir i need Distilled water ratio
first and second flask please it's urgent
I have used double distilled water
Sir after adding congo red, our plate didn't not solidify and also the colour changed from red to blue
Please add 2% agar powder and add Congo Red when the temperature of media comes down to 50-60 °C.
Criticism se Acha hai kbap log khud perform kary. Her kesi k protocol change hoty hai
Kya ap recipe type form me de sakte h
Can you please enlist the ingredients list with specified measurements
Aap kindly video check kijiye recipe diya hua hai 😀
@@thebioscientist ap please mujhe congo red ka bata sakte h 100ml me kya concentration jaye gi?
@@thebioscientistbecause I am a mphil scholar and my research is paused at this point.
@@thebioscientist it would be very helpful to me😇
What did you refer for the making of the video exactly? I mean is it some research paper, and if it is so, can I please get the reference? Its pretty urgent for me and will of be a lot helpful if you provide me one.
Yes I'm providing you the paper link.
pubmed.ncbi.nlm.nih.gov/21860999/
This would be helpful. Thank you for the query.
@@thebioscientist Thank you so much.☺️
@@thebioscientist oh thanks
Sir one question.. How much solvent u took for congo red solution?
Here I have dissolved the Congo Red powder in 50ml of distilled water.
@@thebioscientist But sir here you have taken 1.6 gm of congo red powder what is correct for 200ml. So why have you take 50ml of solvent?