In autospill I saw that you removed your universal negative after adding an unstained control to an empty channel. do you have to re-assign that sample as the universal negative? If not, then how does compensation get subtracted/what would be the purpose of adding it to an empty channel? Thanks so much for this tutorial! It was super helpful.
Hi Jacqueline, Autospill compensation requires no negative control. An unstained cell control can optionally be added to an empty detector in order to estimate the background fluorescence present in the cells. Then when compensation is performed, the signal from the unstained control present in each other detector will be removed, subtracting out the autofluorescent signal. Learn more about Autospill compensation on our website: docs.flowjo.com/flowjo/experiment-based-platforms/plat-comp-overview/autospill-compensation/
In autospill I saw that you removed your universal negative after adding an unstained control to an empty channel. do you have to re-assign that sample as the universal negative? If not, then how does compensation get subtracted/what would be the purpose of adding it to an empty channel? Thanks so much for this tutorial! It was super helpful.
Hi Jacqueline,
Autospill compensation requires no negative control. An unstained cell control can optionally be added to an empty detector in order to estimate the background fluorescence present in the cells. Then when compensation is performed, the signal from the unstained control present in each other detector will be removed, subtracting out the autofluorescent signal.
Learn more about Autospill compensation on our website:
docs.flowjo.com/flowjo/experiment-based-platforms/plat-comp-overview/autospill-compensation/
good tutorial
Мне обманывали мошенники