Hi all, I use this video for my undergraduate teaching. Sorry I've been out of touch but my account was disabled. I'm back in action and hope to be posting great clinical microbiology learning video on a regular basis from now on.
Hi, this is a very good question. It depends how certain you want to be about sterility. Boiling ensures all lumps are disolved. If lumps remains during autoclaving, the centre may not sterilize due to its viscous nature. Manufacturers also recomend you add half the water, then powder then rest of water to wash down sides of the flask so that no small lumps above solution and possibly don't get sterilized. Most clinical labs that make their own agar plates don't bother with the pre-boiling step.
Thank you for your feedback. I like your question as I am reminded of the issues that are forgotten when non-microbiologis make agar. You need to consider how 'defined' your media is. If you were doing an experiment and wanted to control what your insects were eating then you would want to make sure it was not partially degraded by contaminants. Some contaminants grow so slowly you may not realise how degraded your media is. Also don't keep the agar hot for long as this will also degrade it. :-)
Ahh, so to reduce turbidity in the agar so that you can view colony growth and for sterility, would it also be boiled to keep the mixture in liquid form until you autoclave, or is room temperature not cool enough for the solution to harden, thanks for response btw, really helpful
This is an interesting question. Most laboratories have there coat racks just as you come into the lab so the first thing you do is put on your coat. When leaving the lab the last thing you do is take off your coat. To keep the ties clean you must wash your hands before taking off the coat.
Thanks for uploading this video, it's great. You may not know this if you're a microbiol but do you think the procedure would need to be so strict about preventing contamination if you were making media for insects? Thanks.
It depends on the formulation of the agar base. On the side of the container you are told how many grams per 1 litre. So just divide that weight by 40 for 25 mls. Note: be careful autoclaving small quantities of agar, the percentage loss of water through evaporation will lead to overly concentrated solutes, cheers
Thanks for posting this! It is really very helpful. It seems that you are faking yor accent though but I love it too hehehe! Have a nice day and good luck to your career!
This is very interesting and I could just about follow it from the actions, even though, with a UK ear, I could hardly understand a word of what she said. The background noise made it very hard to hear what she was saying. Intelligent questions, too. I shall watch a few times and probably get used to her accent. Thanks for posting.
Good question. Red blood cells are very interesting cells. Since they have lost their nucleus they are probably not metabolically active enough for viruses. There are viruses that will grow on agar plates as long as there are bacteria on the surface. These viruses are called bacteriophages.
many labs prefer sheep blood in alsevers for the same reason. I would say though that horse blood can generate the same results at a quarter of the cost.
OH we see now!! hahaaha We subscribed to your page you only did 12 videos but we will watch them all. I have a vaccum pump but not a vaccum flask for it yet. Thanks for the quick reply my wife says to ask how many time do you wish you could click your fingers and make rude dudes out here disappear haahah Thanks again
Viruses don't take up nutrients, they do not metabolise. New virus particles are made by the host cell, just like how the cell makes proteins and new nucleic acids by reading genetics information, in this case they read the viral nucleic acid instead of the host cell nucleic acid. Influenza virus cannot grow in red blood cells as they are metabolically inactive. You need to get chicken aggs that are fertle and have clees grwong inside the. Influenza virus can be grown in such eggs, not agar.
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Hi all, I use this video for my undergraduate teaching. Sorry I've been out of touch but my account was disabled. I'm back in action and hope to be posting great clinical microbiology learning video on a regular basis from now on.
Hi, this is a very good question. It depends how certain you want to be about sterility. Boiling ensures all lumps are disolved. If lumps remains during autoclaving, the centre may not sterilize due to its viscous nature. Manufacturers also recomend you add half the water, then powder then rest of water to wash down sides of the flask so that no small lumps above solution and possibly don't get sterilized. Most clinical labs that make their own agar plates don't bother with the pre-boiling step.
I had know idea agar plates where made this way, thanks heaps!
Thank you for your feedback. I like your question as I am reminded of the issues that are forgotten when non-microbiologis make agar. You need to consider how 'defined' your media is. If you were doing an experiment and wanted to control what your insects were eating then you would want to make sure it was not partially degraded by contaminants. Some contaminants grow so slowly you may not realise how degraded your media is. Also don't keep the agar hot for long as this will also degrade it. :-)
Ahh, so to reduce turbidity in the agar so that you can view colony growth and for sterility, would it also be boiled to keep the mixture in liquid form until you autoclave, or is room temperature not cool enough for the solution to harden, thanks for response btw, really helpful
Thanks for the great detail in technics
This is an interesting question. Most laboratories have there coat racks just as you come into the lab so the first thing you do is put on your coat. When leaving the lab the last thing you do is take off your coat. To keep the ties clean you must wash your hands before taking off the coat.
Why is the mixture boiled before putting in autoclave, and what woudl have happened if you did not boil it?
Thanks for posting this video. But i have a question, can we use another blood, like chicken blood for example? Thx
Do you have videos on how to stain a slide?
Yes, but I want to add a little footage on tips to preparing a smear so I'll do this in the next month and upload by the end of January!
Thanks for uploading this video, it's great. You may not know this if you're a microbiol but do you think the procedure would need to be so strict about preventing contamination if you were making media for insects? Thanks.
It depends on the formulation of the agar base. On the side of the container you are told how many grams per 1 litre. So just divide that weight by 40 for 25 mls. Note: be careful autoclaving small quantities of agar, the percentage loss of water through evaporation will lead to overly concentrated solutes, cheers
Quite snapping. Is that really necessary?
Thanks for posting this! It is really very helpful. It seems that you are faking yor accent though but I love it too hehehe! Have a nice day and good luck to your career!
what is agar suppose to be used for?
What type and size of autoclave and Company Name plz
This is very interesting and I could just about follow it from the actions, even though, with a UK ear, I could hardly understand a word of what she said. The background noise made it very hard to hear what she was saying. Intelligent questions, too. I shall watch a few times and probably get used to her accent. Thanks for posting.
you click your fingers really well, but why? where would one get horse blood? cool video too hahah Thanks for posting.
Good question. Red blood cells are very interesting cells. Since they have lost their nucleus they are probably not metabolically active enough for viruses. There are viruses that will grow on agar plates as long as there are bacteria on the surface. These viruses are called bacteriophages.
Why did you add horse blood, is it for colouring?
that makes it the name. blood agar. the other component is to serve as an enriched substance for the blood.
many labs prefer sheep blood in alsevers for the same reason. I would say though that horse blood can generate the same results at a quarter of the cost.
OH we see now!! hahaaha We subscribed to your page you only did 12 videos but we will watch them all. I have a vaccum pump but not a vaccum flask for it yet. Thanks for the quick reply my wife says to ask how many time do you wish you could click your fingers and make rude dudes out here disappear haahah Thanks again
Hi, not sure I understand what it is you want. Can you be more specific?
Viruses don't take up nutrients, they do not metabolise. New virus particles are made by the host cell, just like how the cell makes proteins and new nucleic acids by reading genetics information, in this case they read the viral nucleic acid instead of the host cell nucleic acid. Influenza virus cannot grow in red blood cells as they are metabolically inactive. You need to get chicken aggs that are fertle and have clees grwong inside the. Influenza virus can be grown in such eggs, not agar.
Too much background noise but the content is okayyyyyyy.
Yes, made a long time ago. Perhaps this summer I can do an edit. Thanks for watching :-)
All agar does is make it congeal.
agar.io lol
The background noise is horrible. Had to stop watching
*quit lol