PCRプライマー設計ツール Primer3の使い方 2011

[@legihs]

パラメータの詳説があると嬉しいです。

[@aheebru77]

Thanks lots, Sir

[@aheebru77]

One question more some gene sequence show llike example CDS: NP_806485.1
Title: pathogenicity island 1 effector protein
Location: complement(2,862,546..2,864,603)
[Length]
Span: 2,058
Product: 685
if complement sequence, can we direct paste or need to change ?

[@aheebru77]

please tell me , Can i use same setting for q-PCR primer design for bacteria?
Thanks in advance
Ashraf