Sequencing Amino Acids and Edman Degradation

[@athosoliveira1056]

Man, it's amazing the way you organize the board and how you summarize the subjects. Clear and concise!

[@itssomya5860]

Your videos make me realize everytime... Study is not boring.. Our teacher is boring 🤧

[@arkanshsharma850]

As i always say single best great resource for biochemistry

[@irenemutasa7596]

I wish you were my biochemistry lecturer you made this very simple this is of great help

[@befocused71]

Never seen a teacher like him
Your videos makes things really a piece of cake for us

[@brittanyogboghodo8955]

Hi, love all your videos! trying to figure out what video is after this one? Would hate to watch it out of order

[@hayley123-n9d]

I've watched 2 of yours videos so far and I have to say that they are amazing! Your voice is clear, the camera quality is great, you use very easy and understandable terms and you have such a fun and easy way to explain. You've got yourself a new subscriber here! :) Please keep it up. Thank you for all your time and effort.

[@neighborhoodstudent6634]

i love you for doing us a favor and covering most of the lectures taught in class

[@anaherrera4805]

All your videos are awesome!! Thank you! I would loved to had a teacher like you when I went to Medical School.

[@oprospere2568]

quick question: which kind of chromatography is used to remove the (PTH)-amino acid while leaving the shortened peptide chain inside the column?

[@shenjiebao2876]

HPLC?

[@YtVideos-dr2wm]

I guess affinity chromatography with antibody against PTH . BTW u don't know I just guess. I was thinking the same question actually .

[@nicholascirrone4458]

Thank you, you're the only way i can make it through biochem

[@muhammednagas6311]

Wonderful! Many thanks Andrey!

[@devidkardong3122]

Nice illustrations.

[@dzsisters8883]

THANKS !! you saved my life.

[@sadmansakib7405]

Too much useful. Love u teacher.

[@mariaizabelcavassimalves6342]

Very very well explained! I've been watching your lectures to help with my Computational Proteomics Exam :) Thank you very much!

[@refad333]

Superbly described. Thank you for this

[@leewilliam3417]

Great work. Thanks 😊

[@aradhanaprusty884]

I can't thank you enought. You're amazing.

[@kundanlal3328]

I have two questions what's are the amino acid residues for
a) Val-Arg-Gly-Met-Arg-Ala-Ser catalysed by carboxypeptidase A
b) Ala-Lys-Phe- Gly- Asp-Trp- Ser- Arg-Met- Val- Arg-Tyr-Leu-His with Chymotrypsin

[@AKLECTURES]

Do your homework questions!

[@evansonngugi6559]

Great. This has been well put. A big thank you

[@DrSmitPatel]

Ohh sir thanks you what good explaination ever.... ❤️❤️❤️❤️❤️ U sir 🎉

[@sobaah9105]

It is very amazing
But what is meaning by phenyle thiohydantoin??

[@aah2442]

Bless your heart! So well explained

[@EmilyNguyen-ng8fj]

Thank you so much for helping me through this class!!!

[@mdmonirhossain6660]

I am your Big Fan, Sir

[@miguelangelfloresvarela8510]

Great explanation! I really understood it very well, thank you so much!

[@AmirHamza-nz8pb]

Great work💕

[@lakshya3400]

Very nice lecture... thanks

[@thatotlakedi5694]

Good job bro. You know what u're doing and thank you so much for clarifying this.

[@AKLECTURES]

Thato Takedi You're welcome! :)

[@sunidhim.ghatikar3625]

Could you please reveal of how do we identify the AA after labelling n cleaving it off from the main chain? Any device?

[@vibhavk8843]

sunidhi sunanda chromatography

[@codydrew1955]

Your video helps a lot! Wish me luck for my biochemistry test tomorrow! :D

[@AKLECTURES]

cody drew Good luck Cody, let me know how it goes!

[@codydrew1955]

piece of cake! the questions are simpler than i thought

[@AKLECTURES]

cody drew awesome, congrats :)

[@rajivYadav1960]

Sir What is the best method to Extract amino acids from hair in easy language ?
I am interested it for gardening.

[@mamtadubey5689]

Wow it's amazing

[@95felicia]

very good explanation

[@deniz.d2776]

You are amazing sir ; THANK YOU

[@saketpandey3889]

Thank you so much for this u made my day worth of learning ❤️

[@tinatina-tu4mb]

are the SANGER, EDMAN, dansyl methods active on a cyclic polypeptide or not??? thank you for the amazing explanation

[@sameerarshad5236]

Very well explained!

[@ryancornell1637]

You're a great teacher. Thanks!

[@jennyphan3649]

I thought that the edman's degradation happens under mild alkaline conditions not acidic......

[@ti_hani]

Very well explained. Thank you!

[@ZoologyMantra]

sir u r an amazing teacher

[@karthikgvaidya8322]

nice videoQuick question here what about the last amino acid? I mean how to determine that?And also how to practically separate a dth amino acid please reply asap

[@kainonghiwete6937]

thank you so much. You are amazing at this.

[@maggieg.6407]

this is so great! thank you so much

[@maheendhanani4212]

you are an amazingggg teacher!!!

[@seaweedaf]

I’m confused, why wouldn’t the amino on alanine have a positive charge? Every other diagram of alanine shows three hydrogens= positive charge.....

[@selinebrady9667]

Thank you this was rly clear

[@nawalahmed6584]

can someone please answer this?
Q- Why does the Edman’s reagent bind only to the N-terminal amino acid?

[@spintius3752]

You are the man!!! keep up the good work.

[@aliameenful]

tons of thanks.. amazing

[@VikasKumar-wt8tq]

Clear cut

[@Salonisharma1204]

Thank u sir you are amazing 😎

[@pedroaquino6391]

thanks!

[@thisuserlovesmessi]

Life saver

[@abdurrehman7985]

What about the charged amino acids eg: histidine

[@zinab5553]

wow... Awesome

[@AKLECTURES]

+Zinb wehishi thanks! :)

[@heenathakkar5779]

Thanks!

[@freedom21h]

Thank you!

[@sakillaskar3151]

Too good sir .... 👌👌

[@AlvaroAvalos]

Thank you so much!

[@dananjanirathnayake3793]

Awesome.........!!!

[@sabinahasanova7198]

Genius!

[@maleehaaamir665]

thanks!!

[@mustkhdemgoogle9125]

Where's the mechanism of reactions above ?

[@elalamifatimazahra5716]

THANK YOU !

[@Barry88487]

Thanks sir

[@alevelsdemystified3410]

Wow really helpful!

[@adelizarealingo734]

THANK YOU SO MUCH! 💕

[@aymendhifallah1070]

well done man !!!

[@kareemjeiroudi1964]

Perfect!

[@junczhang]

well done!

[@medizzyie]

god bless u

[@MrSVTc0bra]

@5:25 i believe you mean PTC-amino acid

[@xiaowuji]

Umm, no.
The labelled amino acid is cleaved derivitized with PTH (phenylthiohydanotoin) for further analysis.

[@OboeFiles]

how does CNBr cleave?

[@joshuamacks6087]

It cleaves directly after Met.

[@nighthowlerx]

Thanks:D

[@farukkml]

THAAAT, GOOD JOB !

[@akhilmeleka3394]

Plz clear look

[@souravchoudhary9215]

Wow😍

[@lyubomirgizdarski8191]

Are you Russian by any chance? :D I think I hear a small Russian accent