Steps in Gene Cloning || A Complete Comprehensive Concept Video
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- Опубликовано: 7 авг 2024
- A quick revision of 5 Steps in Gene Cloning covering all major aspects of gene cloning.
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00:00|| Introduction
00:08|| What is Gene Cloning?
01:18|| 5 steps in Gene Cloning
01:57|| Step 1: Identification & Isolation of Gene of interest
02:48|| What is Genomic library?
03:29|| Step 2: Insertion of this isolated gene in a suitable vector
03:38|| What is a vector?
05:25|| What are Restriction enzymes?
07:00|| What is ligase?
07:55|| Step 3: Introduction of this vector into a suitable host; E.coli
08:18|| Different gene transfer methods
09:28|| Step 4: Selection of the transformed host cell
10:15|| How antibiotic selection medium works?
15:04|| Step 5: Multiplication or Expression of desired gene in the host
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