How a gene gun works

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  • Опубликовано: 17 окт 2024
  • Kyle Taylor from the Glowing Plant project explains how a gene gun, which is used to transform cells with synthetic DNA, works.

Комментарии • 56

  • @tahiruibrahim7012
    @tahiruibrahim7012 Месяц назад +1

    I have a lot on my mind to achieve in this field, just hoping one day I will get the opportunity to join a lab acquire knowledge and bring it out to solve those problems 🙏🏾

  • @birk_lab4805
    @birk_lab4805 6 лет назад +58

    You stopped before the bombardment! WHHHHYYYYY??? :,,-(

    • @realryder2626
      @realryder2626 2 года назад +1

      Obviously he got a txt and had to reply

  • @JulianoVitiello
    @JulianoVitiello 11 лет назад +7

    Here is a good tip: wrap electric tape on the top part where you screw in the rupture discs. That way it wont sound like nails on a chalkboard each time.

  • @DipanshuGhosh
    @DipanshuGhosh 4 года назад +21

    You should have demonstrated the whole process. You didn't fire the nano particle coated with preferred DNA.

    • @lolpotato
      @lolpotato 2 года назад +4

      Generally gold or tungsten with attached desired gene is used to fire into the vector. This is what i have been taught but never saw it in practical

    • @Noveshd
      @Noveshd Год назад

      ​@@lolpotato yep its only done when u choose biotechnology as career.....not in medical also

  • @lakshayaindrapugal542
    @lakshayaindrapugal542 Год назад +8

    Thank you soo much. Actually I was thinking how does this thing work?. It was referred as " bombarded " in my book 😂.

  • @mewantbrains
    @mewantbrains 9 лет назад +45

    I believe in the video you said you will demonstrate it firing
    I don't see that anywhere in the video

    • @DJMouthwash
      @DJMouthwash 9 лет назад +1

      ***** 0:28 seconds.

    • @mewantbrains
      @mewantbrains 9 лет назад +1

      That's where he says he will demonstrate the firing process but it did not actually fire

    • @TAXABiotechnologies
      @TAXABiotechnologies  8 лет назад +13

      It does fire, it's just not that dramatic!

    • @ashdroid
      @ashdroid 7 лет назад

      The machine is turned off and not running at that specific time 0:28, it even has the door open.
      EDIT: I just saw your other video.

    • @abefromansausagekingofchic6480
      @abefromansausagekingofchic6480 6 лет назад +1

      Riddlemethis yea I was kind of blue balled too. Found the other video though

  • @callahancovington4278
    @callahancovington4278 8 лет назад +9

    It would be nice if there was a laser to help you align the tissue directly into the gun's trajectory.

  • @adventuresoflife486
    @adventuresoflife486 2 года назад +3

    If there is no vector present we use Gene gun/bolistic for plant and microinjection for amimal... 🌷❤️❤️

  • @new_contents_all_day
    @new_contents_all_day 6 лет назад +1

    Can u combine mamels with plants

  • @blazearmoru
    @blazearmoru 11 лет назад +3

    aww. you didn't show us the process with the onion D:
    i hope it's in the next video ^~^

  • @kellycarpenter9350
    @kellycarpenter9350 3 года назад

    Hey I would like to learn how to inject flavonoids into plant tissue to create flaver into plants .like strawberries..I want to cross flaver with pink pepper etc. Kel

  • @Ekergaard
    @Ekergaard 11 лет назад

    I have heard a Ph.D. student say: "This is our nanodrop there're many like it but this one is our". So it's temting to ask if anyone has said: "this is our gene gun ..."

  • @PrasetyoMuhammadDwiBiology
    @PrasetyoMuhammadDwiBiology 7 лет назад +12

    real men use heatshock :D

  • @NavinJohnson_thethird
    @NavinJohnson_thethird 3 года назад

    Dr. John Sanford waa the primary inventor of the gene gun. A former Atheist who became a Christian.

    • @David-ck3gv
      @David-ck3gv 2 года назад

      The prototype was also just a .22 caliber that shot blanks into plant tissue lol

  • @jazzmessenger8
    @jazzmessenger8 10 лет назад +2

    What genes were on the tungsten?

    • @jayesper4390
      @jayesper4390 6 лет назад

      Bill Ricci Dunno, but gold's the way to go. Tungsten should be out of the question.

  • @sapnamenghwar8219
    @sapnamenghwar8219 2 года назад

    Oh I was waiting for them to bombard the tissue, just to see the machine in action 🤔

  • @FoxCurtailed
    @FoxCurtailed 11 лет назад +1

    It looks like a tiny, old school gas pump.

  • @KaitokiNohara
    @KaitokiNohara 4 года назад

    Now I know how it works

  • @christurnblom4825
    @christurnblom4825 6 лет назад +5

    politics of gmo vs non gmo aside, I find it humorous that I've actually heard a scientist on the radio say that this is no different than what farmers have done for thousands of years when breading plants for a desired outcome. This, in response to being asked what he has to say to people who are concerned about the safety of gmo's. They weren't taking calls but if they had been my question would have been "What force of nature causes a similar effect to a gene gun and achieves the same result?"
    Whether or not there is net harm to the environment aside, I just found it insulting that he would play on people's ignorance like that.

    • @christurnblom4825
      @christurnblom4825 6 лет назад +1

      So one of those you mentioned, blasts the same "clip" of DNA into other DNA at over 600 mph, blasting apart the DNA while getting random pieces to stick in the desired place? I guess I do have a lot to learn.

    • @christurnblom4825
      @christurnblom4825 6 лет назад

      What are you talking about? Sarcasm is great! Example:
      Damn you for knowing stuff & making me think about what I've said!!
      Sarcasm helps me feel like I'm doing well while your schooling me. ...and it's occasionally funny.
      All I got left is the thing I put aside at first ie my concern that being selective in that process has a different outcome than in nature or even when we selectively breed in the old method because we don't have all the other iterations that would result, out their doing their thing. I doubt we have any tech. that can figure out if those two ways are any more or less harmful to the environment than the gene gun method but my hunch is that it probably puts things out of balance. At the same time, I'm pretty sure that nature will just shake us off or swat us with her tail and go back into balance if we become too much of a problem.

    • @FringeWizard2
      @FringeWizard2 6 лет назад +2

      I don't think there is a problem with genetically modifying organisms but the specific traits they choose to develop in plants are often really stupid traits they should not have aimed for, whether it be through selective breeding or GMO. Stuff like tomatos that produce more crop with the same amount of nutrients; the result is tomatoes that have significantly less nutritional value. GMOs plants the produce their own pesticide are also a stupid "innovation" as pesticides are often (not always) not very good and harm our health. When they try to develop plants with commercial interests in mind it often times has a lot of drawbacks. It's not also just GMO that is bad but many varieties of plant which are grown for commercial production are in my opinion inferior to heirloom varieties developed by gardeners who just wanted a tasty or beautiful tomato.

    • @selenaburke2956
      @selenaburke2956 6 лет назад

      There are foods you eat with transgenes that got there naturally. like potatoes

    • @MinxInSinx
      @MinxInSinx 2 года назад

      @@FringeWizard2 do you think it is ok to genetically modify your genetics & the lineages of your offspring?
      The only way for your dna to change is if you inject it into the user.
      We are what we eat

  • @deborahjudson350
    @deborahjudson350 7 лет назад +1

    It does not ever fire

  • @zombiebillofthedead
    @zombiebillofthedead 11 лет назад +1

    Filming on a phone I see, wouldn't have known if the picture didn't shake and make that vibrating phone sound. Not that it matters.

  • @smilesinger3652
    @smilesinger3652 3 года назад +1

    Guys who are from vyshavi college 😀

  • @axanachernyavskaya3556
    @axanachernyavskaya3556 10 лет назад

    thanks a lot! very useful!

  • @بنتالانبار-ن4ث
    @بنتالانبار-ن4ث 3 года назад

    الي ما يعرف انكلش شلون يتابعة🥺

    • @balbaks
      @balbaks 3 года назад

      بتقدري تدوسي على علامة CC وبعدين روحي اعدادات الفيديو > الترجمات > ترجمة تلقائية، هي فيها شوية تخبيص بس راح تساعد بالفهم

    • @بنتالانبار-ن4ث
      @بنتالانبار-ن4ث 3 года назад

      @@balbaks تطلعلي كتابه انكلش ما يصير عربي ؟

    • @balbaks
      @balbaks 3 года назад

      @@بنتالانبار-ن4ث لا موجود عربي بس ترجمة تلقائية
      بعد ما تدوسي على CC روحي لاعدادات الفيديو (إشارة المسنن) واختاري ترجمة تلقائية
      أنا فاتح من الحاسوب ممكن من الهاتف يكون مختلف

    • @بنتالانبار-ن4ث
      @بنتالانبار-ن4ث 3 года назад

      @@balbaks يمكن الموبايل يختلف راح اجرب الحاسوب شكرا جزيلا الك

  • @biologyinhindiandenglish8787
    @biologyinhindiandenglish8787 4 года назад

    Ho sakta you can follow any responses to you and

  • @ducktheduck9792
    @ducktheduck9792 3 года назад +3

    kuku

  • @timdwyer3516
    @timdwyer3516 6 лет назад

    Anyone here from 7/8 b

  • @anusandhanwithdevantiwari181
    @anusandhanwithdevantiwari181 4 года назад

    Can u tell me hindi

  • @ឃុនលួង-ហ3ខ
    @ឃុនលួង-ហ3ខ Год назад

    PDS1000$$

  • @miguelpatrao8999
    @miguelpatrao8999 3 года назад

    ahhhhhhhhhhh cromo