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Thank you for your in-depth explanation! Regarding the reactivity of ABTS.+, you mentioned it is mainly reactive with antioxidants such as phenols. Do you believe it would still be reactive toward a cyclic structure with only one C=C, such as cyclohexene? Thank you again!
thank you so much for sharing this! I want to ask, we are doing ABTS test in our lab, however. it was quite confusing for us, cuz when we dissolved our sample with ethanol, we got a negative value of %RSA. Is there anything that we could do to improve our results? some of our samples are partially dissolved in water.
Thank you so much for the useful details. Is the Scavenging Ability (%) the same as RSA(%)? For example, the Scavenging ability of Trolox after calculation using [(A0-A1)/A0] formula is 15.16%, so can I write it as "RSA(%)=15.16%"?
Hopefully can be used for any experiment .. Provided if you are using for multiple experiments then using the dried samples are better.. Because, each experiment differ in there dissolving medium..
i would like to ask regarding on the sample extracts weight in micrograms. 1 microliter=1000 micrograms so why does your cal. 5 microliter sample=50 microgram?
@puteri indera, Thank you for writing to us. The calculation is for 50% Inhibition concentration (also called IC50 calculation ) of antioxidants in the sample corresponding to the standard Trolox concentration reference. So here there is no quantitative estimation of antioxidants present in the sample. I believe you will refer to topics on the Inhibition concentration 50 / IC50 of antioxidants.
Thank you; for the feedback, As you see before in the experiment we have to generate ABTS_ radical. It can be done by 1) chemical reaction (like with manganese dioxide, potassium persulfate, ABAP), 2)enzymatic reaction (peroxidase, myoglobin) or any electrochemical reactions. and when these generated ABTS+ radicals react with some antioxidants can cause slow reactions; slowly causing the error in readings. I hope I have answered the question..
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Hi, I have a doubt. I work with extractions in different solvents (ethanol, methanol, water) and I have 3 results. If I want to know the IC50 of each of them, how would I do it? Thank you
Current Pharma Research ISSN: 2230-7842 CPR 2(3), 2012, 584-599. 584 Isolation of Natural Products *Sharad Visht, Swati Chaturvedi. Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, NH-58, Baghpat Bypass Crossing, Delhi-Haridwar Highway, Meerut-250005 (UP), India.
Thank you for recognising and in my opinion the solvents were taken according to the polarity/no polarity/hydrophilic/hydrophobic of the solutes which has to be extracted in them, so I hope the solvents & extractions which you are using definitely based on this concept. Now the ic50 calculation is totally independent I mean to say that after the absorption measurement all the parameters which is within the equipment or spectrophotometer and the factors associated with the solute and solvent has been absorbed in the values of absorbence so the ic50 calculation can be done for any of the extractions so what you get the value will be absolutely depending on the absorbence ...
@@LEARNERSBIGBOOK i understand .but when i am performing the same, after addition of ethanol to abts water sluotion to dilute to reach 0.7 then it will fluctuate the absorbance . it will not found stable .
@@chandnipatel9236 Thank you for updating.. for the 1st method of ABTS reagent I think you have used double distilled water to set the absorbance value at 0.70. at 734nm. Yes, it is the 2nd method of ABTS reagent preparation is regularly used and i Have spoken about it in the video.. I believe you ll get results in this 2nd type mixture for ABTS.
@@LEARNERSBIGBOOK if I have made sample in ethanol or methanol then still I dilute the abts radical solution with water or should I dilute with ethanol or methanol
Good evening sir.. I need your help for estimating antioxidants like ascorbate, peroxidase, ascorbate peroxidase, gluthionine reductase, superoxide dismutase, catalase etc from the leaf extract of chickpea..it's my genuine request sir..I m ready to pay for your tutorial..how can approach u.. please let me know
![Blox Fruits Dragon Rework Update [Full Stream]](http://i.ytimg.com/vi/EqDAp8udhm0/mqdefault.jpg)
Its very good slides Mr. Khadar ..
Thank you Mr Basha..
It was very useful information. Thank you
Thank you for your feedback۔۔۔ give a like to the videos and please subscribe to our RUclips channel and speard a word with your friends and work mates...
Thank you soo much for clearing each and every thing plxx a video on FRAP assay plxx plxx
Will do soon
Very useful thanks
Thank you for your in-depth explanation! Regarding the reactivity of ABTS.+, you mentioned it is mainly reactive with antioxidants such as phenols. Do you believe it would still be reactive toward a cyclic structure with only one C=C, such as cyclohexene? Thank you again!
thank you so much for sharing this! I want to ask, we are doing ABTS test in our lab, however. it was quite confusing for us, cuz when we dissolved our sample with ethanol, we got a negative value of %RSA. Is there anything that we could do to improve our results? some of our samples are partially dissolved in water.
send me the details of the experiment and calculations..
@@LEARNERSBIGBOOK can I share via email? may i get your email address,please. thank you.
Thank you so much for the useful details. Is the Scavenging Ability (%) the same as RSA(%)? For example, the Scavenging ability of Trolox after calculation using [(A0-A1)/A0] formula is 15.16%, so can I write it as "RSA(%)=15.16%"?
Thank you for your nice video. Where can I cite this method in your journal?
Well done Sir you have done a gr8 job. Sir kindly clear my doubt how 5 microliter is equal to 50microgram
It was really good explanation.
Thank you.
Can we use the same sample which we prepared in DPPH assay for ABTS assay?
Hopefully can be used for any experiment .. Provided if you are using for multiple experiments then using the dried samples are better.. Because, each experiment differ in there dissolving medium..
Okay sir. Thank you for the clarification
@@elizabeththomas4815 you are welcome
i would like to ask regarding on the sample extracts weight in micrograms.
1 microliter=1000 micrograms so why does your cal. 5 microliter sample=50 microgram?
@puteri indera, Thank you for writing to us.
The calculation is for 50% Inhibition concentration (also called IC50 calculation ) of antioxidants in the sample corresponding to the standard Trolox concentration reference. So here there is no quantitative estimation of antioxidants present in the sample. I believe you will refer to topics on the Inhibition concentration 50 / IC50 of antioxidants.
Thank you. Can you explain more the first disadvantage?? it is unclear.
Thank you; for the feedback, As you see before in the experiment we have to generate ABTS_ radical. It can be done by 1) chemical reaction (like with manganese dioxide, potassium persulfate, ABAP),
2)enzymatic reaction (peroxidase, myoglobin)
or any electrochemical reactions.
and when these generated ABTS+ radicals react with some antioxidants can cause slow reactions; slowly causing the error in readings.
I hope I have answered the question..
sir please post one video for ORAC assay.
Thank you for liking our videos.. and please subscribe channel and spread a word among your friends and the classmates or your workmates... And we will try our best 2 prepare the topics asked by you...
Sir instead of trolox can we use ascorbic acid as a standard?
Trolox is very used standard for this ABTS antioxidant scavenging activity...
And you can try with ascobic acid..
Why you have not used control as 0.7 instead of 1.3 as u mentioned
Hi, I have a doubt.
I work with extractions in different solvents (ethanol, methanol, water) and I have 3 results. If I want to know the IC50 of each of them, how would I do it?
Thank you
Current Pharma Research
ISSN: 2230-7842
CPR 2(3), 2012, 584-599.
584
Isolation of Natural Products
*Sharad Visht, Swati Chaturvedi.
Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, NH-58,
Baghpat Bypass Crossing, Delhi-Haridwar Highway, Meerut-250005 (UP), India.
Thank you for recognising and in my opinion the solvents were taken according to the polarity/no polarity/hydrophilic/hydrophobic of the solutes which has to be extracted in them, so I hope the solvents & extractions which you are using definitely based on this concept. Now the ic50 calculation is totally independent I mean to say that after the absorption measurement all the parameters which is within the equipment or spectrophotometer and the factors associated with the solute and solvent has been absorbed in the values of absorbence so the ic50 calculation can be done for any of the extractions so what you get the value will be absolutely depending on the absorbence ...
www.researchgate.net/deref/http%3A%2F%2Fwww.slideshare.net%2Frahulbs89%2Fextraction-of-plant-contituents
Sir, the scavenging ability is 57% at 1250 μg/ml. But when i calculate IC50, it shows 2.722 µg/mL. How is it possible?
i am suffuring from dilution problem with ABTS absorbance to set at 0.7 at 734 nm.please help me
You mean you can't understand during the explanation in the slide?
@@LEARNERSBIGBOOK i understand .but when i am performing the same, after addition of ethanol to abts water sluotion to dilute to reach 0.7 then it will fluctuate the absorbance . it will not found stable .
i will try for 7 mmole to 140 mmole ratio of concentration of ABTS and potassium persulfate
@@chandnipatel9236 Thank you for updating.. for the 1st method of ABTS reagent I think you have used double distilled water to set the absorbance value at 0.70. at 734nm.
Yes, it is the 2nd method of ABTS reagent preparation is regularly used and i Have spoken about it in the video.. I believe you ll get results in this 2nd type mixture for ABTS.
@@LEARNERSBIGBOOK if I have made sample in ethanol or methanol then still I dilute the abts radical solution with water or should I dilute with ethanol or methanol
Good evening sir..
I need your help for estimating antioxidants like ascorbate, peroxidase, ascorbate peroxidase, gluthionine reductase, superoxide dismutase, catalase etc from the leaf extract of chickpea..it's my genuine request sir..I m ready to pay for your tutorial..how can approach u.. please let me know
Plz upload a video of this test performance for more guudence
Thank you for writing to us.. surely will do it...
감사합니다 ㅎㅎ
you are most welcome.. request you to subscribe to our channel and to spread a word of about our youtube channel in your workmates..
Method1 didn't work for me also, I think I'll try method 2
Can you send a reference?
Thank you soo much for clearing each and every thing plxx a video on FRAP assay plxx plxx
Will do soon