Both ELISA and Western blot use antibodies to detect proteins. ELISAs require an antibody pair where both antibodies detect the target protein to provide a signal. A western separates proteins by size and uses one antibody to detect the protein and another antibody to detect that antibody. The ELISA and IQELISA technique uses a 96-well platform that is typically more user friendly and allows more sample throughput per experiment. Additionally, quantitative data can be obtained on a routine basis with ELISA and IQELISA whereas with the western blot it can be more demanding.
![Sealing the plate [qPCR]](http://i.ytimg.com/vi/dXfvsBH5Lt4/mqdefault.jpg)
![Sealing the plate [qPCR]](/img/tr.png)
How does it compare to western blotting????
Both ELISA and Western blot use antibodies to detect proteins. ELISAs require an antibody pair where both antibodies detect the target protein to provide a signal. A western separates proteins by size and uses one antibody to detect the protein and another antibody to detect that antibody.
The ELISA and IQELISA technique uses a 96-well platform that is typically more user friendly and allows more sample throughput per experiment. Additionally, quantitative data can be obtained on a routine basis with ELISA and IQELISA whereas with the western blot it can be more demanding.