My teacher recommended your Microbiology videos since the COVID 19 made our classes online for the remainder of the semester. Thank you your info was very very valuable and your calm collected voice made it easy to follow. Thank you!
not sure if you guys gives a damn but if you are stoned like me during the covid times then you can stream pretty much all of the new movies and series on instaflixxer. Been streaming with my brother during the lockdown =)
Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?
I have a question if we found that all the antibiotic give susceptible result, which one will be the best antibiotic that can be prescribed to the patient?
Is it possible to do this method with unknown bacteria? Let's say you go out and collect bacteria from a park bench, then plate the bacteria, isolate individual colonies, and then expose each colony to this method? My concern would be you could test the unknown bacteria against an antibiotic that does not kill that type of bacteria and then call it resistant. Any suggestions?
First and foremost, it is not a good idea to collect unknown bacteria from a park bench or any surroundings as you could easily end up collecting virulent pathogenic strains which would be a serious biohazard for all experimenters. Nevermind, proceeding with the unknown bacteria idea which is a nice idea you have ,let's answer the remainder of your question. So let's say we have isolated an unknown bacteria which is non-pathogenic to Homo sapiens and exposed it to the antibiotic using the method discussed above. We may find that there is no zone of inhibition and thus we would say that the antibiotic is "ineffective" against this unknown bacteria. Antibiotics whether both lytic or static ,have targets in the bacterial cell and the absence of these targets renders them powerless to affect a bacterial cell. So your unknown bacteria would simply not have the target used by the respective antibiotic to affect it. Resistance on the other hand is when you have a known bacteria which was always being inhibited by the antibiotic and then after some time, you find a colony that grows uninhibited . This would be termed as "resistance" . We would say that the members of the colony gained " resistance" against that antibiotic by mutation.
you the GOAT for this video, very self explanatory with perfect reasoning and understanding verbally!!!!!!
My teacher recommended your Microbiology videos since the COVID 19 made our classes online for the remainder of the semester. Thank you your info was very very valuable and your calm collected voice made it easy to follow. Thank you!
not sure if you guys gives a damn but if you are stoned like me during the covid times then you can stream pretty much all of the new movies and series on instaflixxer. Been streaming with my brother during the lockdown =)
@Canaan Brixton yup, have been using instaflixxer for since november myself :D
@Canaan Brixton Yup, I have been watching on instaflixxer for since november myself =)
My teacher linked this video in a question on one of my assignments. Super cool, super informative, and your voice is very pleasant to listen to.
Thank you!!!!! saving me one hour before going to exam!!!!!
Wow!!!! How awesome it is to be able to understand something in less than 10 min!! These videos are great !!!!
Thank you so much!!! your videos are very helpful and the best. You always make it easier to understand in only few mins!! Thank YOU!!!!!
Well detailed explanations. Simply illustrated. I had a quick understanding of what it is about. Thank you sir.
Awesome thank you. Great way to walk us through it! Doing Microbiology on line during Covid and needed help. 👍🏻🤷🏼♀️
Thank you! thank you! thank you! Literally one of the best channels!
This was very well explained and informative❤️
That's sooooooooooooo helpful! Thanks a lot! ♥
You're welcome!
another great explaining as always thank you so much 🙏🏼
Great video
Thank you so much for this video!
This was so helpful! Thanks
This is helpful! Thanks So much!!
If zones of diameter are the same size which antibiotic would you choose as the best one?
My English not very well, but u made it easy thanks a lots
thank you very much. It makes a lot of sense.
Thank you for sharing!
Where's your link of the past video please hehe
Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?
Thank you sir very useful☺
Good vid.but from where do the numbers that are used as a criteria come from?
I have a question if we found that all the antibiotic give susceptible result, which one will be the best antibiotic that can be prescribed to the patient?
Where did you find the data on the diameters of antibiotics to classify susceptibility? What source or website?
thanks so much your vid was very helpful
Thanks for the comment!
Thanks a lot Really i got lot af adventage about this lesson and i would like to get Report of B+ bacteria culure and also N- bacteria culture
Thnk u very much ❤️ 👌nice explanation.. 💐
So if there is no treatment on the plate but there is a ZOI, would that make it invalid or?
Is it possible to do this method with unknown bacteria? Let's say you go out and collect bacteria from a park bench, then plate the bacteria, isolate individual colonies, and then expose each colony to this method? My concern would be you could test the unknown bacteria against an antibiotic that does not kill that type of bacteria and then call it resistant. Any suggestions?
Great question!. I’m glad to see that I’m not the only one who inquired about the exact instance.
First and foremost, it is not a good idea to collect unknown bacteria from a park bench or any surroundings as you could easily end up collecting virulent pathogenic strains which would be a serious biohazard for all experimenters. Nevermind, proceeding with the unknown bacteria idea which is a nice idea you have ,let's answer the remainder of your question. So let's say we have isolated an unknown bacteria which is non-pathogenic to Homo sapiens and exposed it to the antibiotic using the method discussed above. We may find that there is no zone of inhibition and thus we would say that the antibiotic is "ineffective" against this unknown bacteria. Antibiotics whether both lytic or static ,have targets in the bacterial cell and the absence of these targets renders them powerless to affect a bacterial cell. So your unknown bacteria would simply not have the target used by the respective antibiotic to affect it. Resistance on the other hand is when you have a known bacteria which was always being inhibited by the antibiotic and then after some time, you find a colony that grows uninhibited . This would be termed as "resistance" . We would say that the members of the colony gained " resistance" against that antibiotic by mutation.
You are the best!
Thank you!!
Glad you liked this video!
May I ask you how to access CLSI on AST?
In real life, the zone of inhibition is distorted and not a perfect circle as illustrated here. How would the diameter be calculated then?
Kirby was the scietific? I can't find photos of his face.
thank youu for this
thank you finally understand
Tq.. Sir nice teach
Thank you
im here just by curiosity i have just. learn i dint even take this class lol
nice!!
Thx alot
No problem!