Top 6 Steps to Run The PERFECT Column Chromatography

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In this video, Dr. Andrew Grant demonstrates how to successfully run a column chromatography.
Music: All That by Bensound
#SCIENCE

[@ericlarsen4758]

Great video!!! It surprises me to this day how few (good) examples of column chromatography there are on youtube.

[@moaadbalaban7535]

Great video and an amazing prof!! 🙂

[@SixTough]

Professor's pippette pullout game: unmatched

[@Pedro-ss2qr]

You can make the eluent run much faster through the burette if you apply some pressure on the top of the column, with the rubber bulb from the pasteur pipettes or a pompette. It saved me so much time 🙏

[@tomastank8763]

awesome like the lab coat :)

[@K1R8Y92]

Correct me if I'm wrong, but a Rf value of about 0.5 of the alcohol in 2:1 hex/EtOAc means you expect the alcohol to elute from the column after just two column volumes of solvent.
From my experience that is way too fast to achieve a proper separation of different compounds.
I usually aim for an eluent mixture with an Rf value of 0.2 or lower of my desired cmpd.
For reaction control etc. however I like to have the spots somewhere in the middle of the TLC plate

[@brothermutant7370]

Very cool. I have a science question: If you had an unknown compound that you could purify using column chromatography, how would one go about determining what the chemical formula would most likely be?

[@elisalai7332]

Run an NMR

[@GamerBraga]

@Brother Mutant73, if you don't know anything about the compound, after purification, acquire the carbon and proton NMR, and HRMS (maybe you'll need to test some methodologies if it is gonna fly on ESI-TOF). In this case, FT-IR will not kill you, run an ATR. After that you'll have lots of data to elucidate the structure, if NMR is cloudy, go for 2D like NOESY, COSY, HMBC and HMQC, etc

[@cyclopentylprime8381]

Upset to see another seemingly inactive science channel. You have a lot of different heads talking, what institution are you guys based at?

[@jaydendoyle6121]

Ware did you go I miss your videos

[@SuzanneRoussin]

1:25 Is the top spot in the 4th TLC 2:1 solvent identified as the ketone because ketone is nonpolar and the solvent had twice as much nonpolar hexane as polar EtOH? Just trying to make sure I understand how you knew that the top spot was the ketone and not the alcohol.

[@sovikdey4287]

I think he didn't see " how ton use fumehood video"

[@williamaniuk948]

I actually understand half of what he's saying