🔬 Oil Immersion Microscopy at 1000x | Amateur Microscopy
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- Опубликовано: 25 июл 2024
- Celebrating 1000 subscribers with 1000x magnification! Exchanging the objective, oil immersion, cleaning the objective and a look at some algae with and without oil.
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I'm impressed at the amount I've learnt about microscopes in just a few days by just watching your channel. Thank you so much for taking the time to make these great videos.
Great to hear!
Hey can u do a video about " how to see cheek cells" ? Can u do it? I have very much interest in seeing it
Gracias por su explicación
I like the light and crisp image you got there. I´m using the aperture to get sharp images - but that kills the light... Are you using any video editing software to get that bright image?
While I can use the video editor to control brightness, I generally don't do that. The camera allows me to adjust the brightness setting by controlling the exposure time and this way I am able to set up everything before starting to film. This also allows me to over and to underexpose as well. I can see the image on the screen of the camera and when everything is fine, I press record.
Ok I'm buying a microscope
whoa, an oil immersion tutorial from Microbehunter that isn't "just don't"?
Hello, my microscope lense title says that it uses water immersion, is this even possible ?
It just not a video bro it tells lots of knowledge 😊😊😊
My oil Emerson lens is showing black dots on even clean slide ? Any idea
If the lens is dirty, then you would probably not see any dots it would be blurry. Check for dirt on the eyepiece.
I kind of want a separate microscope for my oil immersion, to be safe. One thing you didn't mention: aren't you supposed to put oil on the condenser as well and then have it touch the bottom of the slide?
Also possible, but the risk of getting oil in the condenser is too high for me. Possibly there are condensers that are designed for this as well: www.microbehunter.com/microscopy-forum/viewtopic.php?t=3765
If i remember correctly that is only used on high NA condensers mostly for dark field microscopy at very high magnification, one of the applications was to see _Treponema pallidum_ (syphilis bacteria) because its too thin (about 200nm or less) therefore very difficult to see with other light microscopy techniques.
Edit: Indeed you NEED to put oil on the condenser to reach a NA over 1 (only if your condenser actually supports this! do not use oil if your condenser has a numeric aperture of less than 1 or you aren't sure about it) otherwise you wont get a significant benefit using the oil objective as you will be limited to the NA of the condenser which is usually .6 for most budget microscopes with abbe condensers. Dayum it seems its time for me to get a 60x objective and maybe a new condenser, i was looking at some Nikon condensers of NA of .9 (dry) and 1.4 (oil) and they cost $2.3k :(
Update, well my condenser is NA .9 so there is some benefit for me using oil immersion (the lower objective is just NA .65) but the mess of it could be avoided if i had a dry objective with a NA as close as possible to .9 seems i will have to get one.
How can you minimize the likelihood of immersion oil getting on the 40x "dry" objective?
One expedient often used in clinical laboratories is to mount the "dry" 40x on the opposite side of the nosepiece from the 100x oil immersion objective. This mounting arrangement reduces the likelihood of inadvertent dipping of the 40x "dry" objective into immersion oil as you rotate the nosepiece between the 100x oil objective and the 40x "dry" objective.
How about we just don’t use oil immersion