How to properly use a compound light microscope

This is one of a series of videos on cell biology. This video will introduce compound light microscopes, their parts, and how to properly use them. Some of the parts I will describe include different objective lenses, the diaphragm, eyepiece, and stage.

[@jyquenix314]

This mans a legend. He's still responding to comments 7 years later.

[@SiouxScience]

Sometimes I miss comments... but I try to reply to everything. I appreciate people taking the time to comment!

[@mihalcescusd]

Omg im in ur class- this is my dads ac thats why the names different

[@TinySkySky]

This video is so helpful. I'm taking MicroBiology right now and this video really helped me with my lab homework today :)

[@SiouxScience]

I'm glad to help! Thanks for watching.

[@mokshitalia4960]

came here from mr miller class

[@alex.park07]

lol

[@sarahd7378]

same here!~~ :D

[@noone-gi6mc]

lmao

[@SiouxScience]

Thanks Mr. Miller.

[@eli_luna3388]

This is a very helpful explanation, thank youuu.

[@SiouxScience]

My pleasure - thanks for the kind words!

[@smeegames5937]

What is the tube that the eyepiece is on called? It's not mentioned in the video.

[@SiouxScience]

I've always called it the body tube. I tried to outline the things that people need to touch and manipulate - sorry to have left it out!

[@gordonchow2831]

Hello quick question is it the coarse adjustment or course adjustment? thanks

[@SiouxScience]

Coarse, as in rough. Not detailed or precise. If I had a typo in there my apologies!

[@kaylinann914]

Thank you so much! I have a test on how to use a microscope and I'm very nervous. I haven't had very much success with microscopes in the past but this has definitely helped.

[@SiouxScience]

+Kaylin Styles I'm glad I could help. You're welcome.

[@nadinhanaysha1363]

When using light microscopy, do you start from the largest or the smallest distance (between the lens and? The specimen)? Why

[@kandiruffner2193]

Largest distance from between the lens and specimen. This would mean that you are using the lowest powered lens, and this would allow you to center the specimen. Once the specimen is centered, than one can change to a different lens, to get a closer look.

[@nadinhanaysha1363]

@@kandiruffner2193 Thank U

[@brylanjones10]

Here from Mrs. Tina’s class

[@SiouxScience]

Thanks Mrs. Tina!

[@patalkalgames9809]

Nice,good

[@devanmitchem8001]

In forensics in high school so this helped thanks

[@SiouxScience]

My pleasure - thanks for watching.

[@beckakhiwu1766]

Thanks for this video

[@SiouxScience]

You're welcome, I'm happy to help!

[@fajardoryana.1845]

Can we use direct sunlight when mirror is the light source? Thank you. 🙂

[@SiouxScience]

You bet. You can then use the diaphragm to change the amount of light you end up with.

[@fajardoryana.1845]

@@SiouxScience Thank you♥️ i'll be back for more questions in the future🤩

[@SiouxScience]

@@fajardoryana.1845 You bet! Glad to help.

[@ULTRASIANGaming]

Um, if a beam of light passed through the mirror, what parts would the light pass through?

[@SiouxScience]

+ULTRASIAN Gaming Bounced off a mirror through the diaphragm. I'll have to take a look through and see if I misspoke, thanks!

[@biologieeemland8141]

do you have video's how to repair a microscope

[@SiouxScience]

No, I’m sorry!

[@angelcorpuz1122]

This was reaaaaaaalllllyy helpful. Thank you so much, Sir.

[@SiouxScience]

I'm glad I could help! You're welcome.

[@superquizers9500]

this is awsome

[@SiouxScience]

Thanks!

[@lexsiepatterson5730]

hey what’s the name for the parts labeled 4 and 5 i can’t find it anywhere

[@SiouxScience]

Low and high power objective lenses.

[@lexsiepatterson5730]

@@SiouxScience thank you so much!

[@hunterlynnecoonrod3524]

Hi, I couldn’t find 7 what is it?

[@SiouxScience]

I think it is the diaphragm, that controls how much light gets to the thing you're looking at.

[@rishabhbehrani5934]

cool its intresting to learn like this

[@SiouxScience]

+Rishabh Boy Thanks! I hope it helped.

[@meinbuch9458]

BiologyMonk Thanks for uploading this video,it helped me a lot! 😃

[@SiouxScience]

You're welcome! I'm glad to help.

[@meinbuch9458]

BiologyMonk I bought my own compound light microscope a few days ago,and i'm fascinated by what i can perceive when i'm using it.Your video certainly assisted me in operating it.

[@SiouxScience]

Thank you! I'm glad to help.

[@leobluesy]

Interesting.. thanks

[@SiouxScience]

Thank you - I appreciate it. Glad to help.

[@nggt3912]

Hi people in ms nippert or killian class I’m johnny

[@SiouxScience]

Thanks Ms. Nippert and Killian.

[@shameless4126]

Here from Mr. Casserole’s class 😔🤚

[@SiouxScience]

Thanks Mr. Casserole!

[@peachyaesthetic9248]

hi people in ms reilly's class

[@SiouxScience]

Thanks Ms. Reilly.

[@Verse28]

Came here for distance learning from Ms. Misuraco.

[@SiouxScience]

Nice. Thanks Ms. Misuraco.

[@biologieeemland8141]

what was you name. i coudn't hear it.
groet jaap smook

[@justinknitter6057]

Thanks

[@laylahqueen1520]

Here from Lincoln middle. WEST VIRGINIA Harrison county

[@SiouxScience]

Nice! Thanks for watching.

[@chooniklee]

My daughter says thanks!

[@SiouxScience]

Tell her I'm glad I could help!

[@RamKumar-os7bk]

super

[@SiouxScience]

Thanks!

[@theonlyminmatters2133]

Coarse adjustment...

[@DarkMindPsycho]

From miss shaju class

[@SiouxScience]

Thanks Miss Shaju!

[@marleyweirHHS]

from ms. nichols' class

[@SiouxScience]

Thanks Ms. Nichols.

[@mokshitalia4960]

what are the answer for 1, 4, and 5

[@olivermarburg]

savage

[@mikesosa937]

Second!

[@dragondude1402]

Hi anyone in Reyes's class!

[@mokshitalia4960]

nah dude i feel like all the veiws for this are all science teachers students. imao

[@ruthbaldner9036]

"Course" focus? Really?

[@SiouxScience]

Of coarse. Whoops.

[@minhajkabir5967]

6:10 yeah i got first hand experience by shattering the entire slide

[@SiouxScience]

It happens. If you feel bad just consider that your teacher has probably broken more glassware this year than you have and they've been doing this for years.

[@minhajkabir5967]

BiologyMonk .thanks . Yeah i feel better already.

[@minhajkabir5967]

BiologyMonk please keep up the good work 🤗
Its rare to find positivity in a youtube comment section

[@britoronto6628]

Ha, Ha, Ha--"course" adjustment is really COARSE adjustment.