CytoSMART Lux3 BR Duo Kit - Accurate tracking of chemotactic cell migration

HeLa cells were seeded in the Ibidi µ-Slide Chemotaxis, with either 20% FBS in one reservoir and 0% FBS in the other, or 10% FBS in both reservoirs. High-quality images were made using the Lux3 BR Duo Kit, enabling single cells to be tracked with FIJI-plugin TrackMate (pink; tracked paths in yellow), as well as direct comparison of the experimental groups. Total covered distance was smaller with the FBS gradient, but those cells displayed a preference to migrate towards the higher FBS concentration.