In a Chemostat, change in biomass (increase or decrease in microbes number) is related to how fast the microbes grow and what is the concentration of the medium in the vessel. The change in biomass with time can be defined by the following equation dx/dt =uX - DX Where, u is specific growth rate, D is the dilution rate, X is the cell mass. At steady state, means the number of microbes is fixed or constant in the vessel over time or dx/dt =0 So, 0 = uX - DX Or uX = DX or u = D. So, if you can find out the u ( it is generally calculated from the g) you will know D also (u=D). Then you can use D=f/V equation.
Thank you for your comment. I will try to upload more videos like this within Jan. So, you don't have to search a lot for this numerical problems. Like, bacterial genetics, thermal death, technique, RDT etc. Year wise and section wise. Best of luck for the GATE exam.
Sir please solve this question ( From GATE 2020). A 100 ul aliquot (10^-4 dilution) of bacterial culture plated on agar gave 4 colonies tha bacterial strock concentration is ?
Pardon me if I'm wrong but i think here ( 4:01 ) you have done mistake..
Yes you are right. I have multiplied 50 X 60 and write 300 instead of 3000
So, if t = 3000
Your answer will be 1X2^100.
Thank you for your comment.
Sir, also I didn't understand this ( 5:02 ) question, please explain.
In a Chemostat, change in biomass (increase or decrease in microbes number) is related to how fast the microbes grow and what is the concentration of the medium in the vessel. The change in biomass with time can be defined by the following equation
dx/dt =uX - DX
Where, u is specific growth rate,
D is the dilution rate,
X is the cell mass.
At steady state, means the number of microbes is fixed or constant in the vessel over time or dx/dt =0
So, 0 = uX - DX
Or uX = DX
or u = D.
So, if you can find out the u ( it is generally calculated from the g) you will know D also (u=D).
Then you can use D=f/V equation.
@@DrNirmalyaGhoshal thank you sir, its an amazing video for practice.
Thank you for your comment. I will try to upload more videos like this within Jan. So, you don't have to search a lot for this numerical problems. Like, bacterial genetics, thermal death, technique, RDT etc. Year wise and section wise.
Best of luck for the GATE exam.
Thank you sir
Thank you for your comment.
Feel free to write your opinion here.
thanks sir for these videos! keep going👍
Thank you for your comment.
Sir at 5:09 time the ans should be D right? It is asking per hour no. Of generation
5:05 - answer is 231
In, 2021, Q.11 the P:O in Z mobilis is 1 not 2. So, Y will be 8.6 not 4.3.
So, correct options is D.
Sir in exam how will we get to know about P:O of microorganisms ? Will it be given ?
It was not provided in the exam. I know it is little bit though for the students to remember the P:O ratio for all organism.
Sir, in the first question of 2021, how are you getting 2 moles of ATP each to begin with the sum?
Glycolysis has a net yield of 2 ATP and 2 NADH for every one glucose molecule processed.
@@DrNirmalyaGhoshal Ohh Yes, Thank you sir!!
Sir in question 18!
(miu=D in steady state is fine) But which is that formula Y = F × miu ? Kindly elaborate a bit!
There was a mistake in this solution and that's why this part is deleted from the video. Thanks for commenting. I will solve the problem in future.
Sir please solve this question ( From GATE 2020). A 100 ul aliquot (10^-4 dilution) of bacterial culture plated on agar gave 4 colonies tha bacterial strock concentration is ?
Wait for 3 to 4 days. Dilution, stock solution related problems will be discussed there from the previous year questions paper. It is almost finished.
👍
Sir at 6:00 min time should be 3000 not 300
Yes, you are right