Dilution of primers to 100 µM master stock

Form 100 µM master stock the 10 µM working stock can be prepared by using 10 µl of master stock and 90 µl of sterile distilled or PCR grade water in sterile eppendof tubes
Thank you !!!!

[@Versatilelife007]

Thank you so much

[@reshna4387]

Great share!! Keep doing 👍

[@aparnakhairnarprincipal9279]

Very nice, Amrut💐👌🏻👌🏻👌🏻👏🏻👏🏻👏🏻

[@gazelgaddafi659]

Informative!👍

[@sidhu_priya936]

He seems knowledgeable but lukin nervous.. Informative video. Thanks.

[@lifereplicon]

It was first video without retake 🥲
Thank you 😊

[@jaseelmx5312]

Nicely explained ❤❤ 🙌

[@vinayakmohandas1081]

Great video bro.......👍👍

[@saviofranklin7903]

Super Bhaijaan ❤️💯

[@ujwaladaware9445]

Superb👌👌

[@shuvronilchakraborty8163]

Thank you dada

[@Sagarpatil12654]

Unique 🔥🔥😍😍😍

[@sanithreddy4308]

Super 👌👌

[@amalabenny7343]

Keep doing👍👍

[@mayurthesiya5734]

great

[@gourangacharantripathy9144]

sir please post a video on how to do master mix and the process after that that will be very helpful.

[@lifereplicon]

Ok

[@nostro1940]

funny p@jeet

[@karunakrishna5953]

👌👌

[@SaumyaSharma123]

Sir my primer is already in 200ul TE so I don’t need to do this right?

[@lifereplicon]

They are already dissolved ?

[@SaumyaSharma123]

@@lifereplicon yeah. They are liquid

[@lifereplicon]

So they are already dissolved hence u don't have to prepare master stock as u just have to dilute to standard 10 um working stock

[@GaneshKunayak]

@@lifereplicon please explain the dilution ratio , i mean how to convert and what is 10 um & 100 um

[@michaelkellya7506]

sorry sir, how did you get that 10 for multiplying with nmol??

[@akshaybvyawahare3363]

Whatever is your nmol multiply by 10 that will give you 100 micromolar

[@manishchand6744]

For how long we can use this stock solution any standard time period ??

[@lifereplicon]

You can use as long as they aren’t contaminated ! 😅😇

[@hidayahramli7824]

Hi. Do you mean the autoclaved distilled water?

[@lifereplicon]

Yes but i will recommend going with commercial avaliable nuclease free water

[@rushikeshchavan8623]

why we multiply the nmol of primers by 10

[@lifereplicon]

Because it will gove you 100 micromolar concentration

[@loverofimrankhan945]

You didn't mention units ul or ml

[@lifereplicon]

Where ?

[@lifereplicon]

Really appreciated pls tell me your query

[@MohammedumarSadiq-sk6wz]

From which college u r bro??

[@MohammedumarSadiq-sk6wz]

Plz reply Kerala agricultural University

[@lifereplicon]

Yes

[@ANIRUDH774]

Primers tirning yelliw from bottom

[@lifereplicon]

Pls elaborate