Two things here: 1. Simply dissolving pencillin won't release the active form. Since it's an oral formulation, it must go through stomach to become active. So, a solution of penicillin won't be effective against bacteria 2. None of our cell has cell wall. Bacterial cell wall has peptidoglycan. Penicillin disrupts synthesis of this chemical. Now fungal cell wall lacks peptidoglycan hence antibiotics aren't very effective against them
Penicillin VK isn't a prodrug, it doesn't need to be activated by first pass metabolism and specifically PVK is significantly inactivated in first pass. It does need the low pH of the GI tract to be properly absorbed but that's not "activation" per se.
And that’s why you have to take a FULL COURSE of antibiotics and not just stop after a few days when your feeling better. It’s to make sure the bacteria is aniahlated or you risk antibiotic resistant bacteria.
You do have to take the full course but that's not why it didn't work in the video. There are two major families of antibiotics: *bacteriocidal* that kill bacteria and *bacteriostatic* that prevent them from multiplying and let your own T-cells do the rest. Penicillin is bacteriostatic. By the way, although the mechanism is called "bacterio"-cidal/static, most antibiotics work on *all* cells.. The bacteria will stop multiplying, but so will your T-cells. That's why it is important to start taking antibiotics only after the symptoms have fully developed, indicating that your body has produced enough T-cells to fight the infection.
No that's not how resistance works. If any resistant bacteria are present they will still be around regardless of whether or not you finish the full course. Stopping it early doesn't magically create resistant bacteria, it just may not kill all the bacteria. It's actually the opposite of what you are saying. If someone is one antibiotics for a long time there is constant selective pressure in favor of producing resistant bacteria.
The penicillin in pills is not per say penicillin. It’s an analogue that needs to pass through the stomach acids and then be activated by cytochromes in the liver to be active
Wow... reading your response makes me feel completely retarded. Full blown retarded. Ill just trust the dude at Walgreens. Not the one who greets me at the counter, but the guy in the white coat way back there, the guy who always tells me that i should ask my doctor for whatever advice i ask him.
It takes 18 to 24 hours for penicillin V to be at 10% it takes three to five days to be at 100% effective... that's why when they do a rapid antibiotic test at the emergency room or doctor's office it can take up to 24 hours to get your results... that's why most doctors will prescribe antibiotics by symptoms and they usually prescribe a spectrum antibiotic... amoxicillin. doxycycline. cephalexin. ciprofloxacin. clindamycin. metronidazole. azithromycin. sulfamethoxazole and trimethoprim..
Its not ineffective, its just that Penicillin only acts with bacterias sensitive to it. Thats why doctors prescribe certain antiobiotics depends on a specific desease.
Gargling salt water gets a lot of its beneficial effect from the way strong salt water will draw water from inside the infected area through ionic osmosis, helping to mechanically flush the bacteria and dead cells outward into your mouth where you spit it out after the gargle.
Yes! My mom too. Everything from doing it every so often just because to tooth problems to sore throats and ulcers. She was right most of the time. I'd still love to see it in action though and hear scientifically why it works or doesn't work.
@@udayvarma756 Unless he's got access to anaesthetic and needles, that's gonna be like looking for a needle in a haystack. Not to mention he'd have a hard time getting a woman to collect all her period blood for a video.
Nice way to learn how medicine works. Would be interested in seeing what a sneeze/mucus looks like, and maybe add some sort of disinfectant (bleach/lysol/peroxide, etc)
He has a video with a sample of something from his throat. It had many different bacteria and body cells (like mucus and white blood cells). You can find it if you scroll through his page :) Quick edit: it’s a sample from coughing I believe
@Walter “The Cat Yeeter” B it's a biodrug, they're sensitive to environmental degradation over time. If it's expired it's no longer capable of doing the job. Sulfonamides are simpler, much more shelf stable antibiotics, and take far longer to degrade in storage.
@@pcproffy No, no, no. It not only prevents them from reproducing, it prevents them from GROWING, as it prevents new cross-links in peptidoglycan / murein, out of which the bacterial cell wall is made. if the cell grows, the cross-links are broken, so the cell wall can expand by integrating new chains of peptidoglycan. but due to penicillin buinding to peptidoglycan, new cross-links between those new and old chains can't be built, so the cell just leaks out. same happens during reproduction, as the cell wall devides during mitosis.
Plus it is a time dependent antibiotic, meaning that it is only effective if the bacteria is exposed to a sufficient concentration of the medication for an extended period of time
You need only take a college course of microbiology to learn about different bacteria and different antibiotics. This is why you are prescribed certain antibiotics depending on your symptoms and also why it is so important to be honest with your Dr about that because different antibiotics work depending on what bacteria you are infected with. That's also why there are "broad spectrum" antibiotics because they will kill any and all bacteria including the natural flora, or natural bacteria that your body creates.
I once had a doctor that did blood samples in his office. Ten minutes later he prescribed exactly what you needed!!! That was 30 yrs ago! Now you have to run all over town to get blood drawn but have already been given drugs
"you *only* need to take a college course of microbiology to..." Yeah i have the sudden urge to learn about Microbes so Lemme reach in my pocket, drop $7000 on a semester of school just so i can scratch my intellectual curiosity RUclips is better my guy
Well here is the deal. Penicillin only works on very specific bacteria. You appear to have rod shaped bacteria which is often something like Bacillus that are in spore form. In addition, penicillin also degrades in efficacy over time. But its doubtful it worked because you gave it to spore formers. I am a microbiologist.
I actually wondered about that - i noticed the small rods - but dismissed my mediocre microbiology education because I'm only an RN and not a Microbiologist.
Yagamil46: (And to anyone else with an allergy to Penicillin) Fun Fact: So the only thing to kill Syphilis is Penicillin. What to do when a pt has Syphyllis but cannot take Penicillin? Pt will be admitted indefinitely wifh a constant IV drip of VERY SMALL AMOUNTS of Penicillin and Epinephrine and fluids until the pt has become - for lack of a better word /explanation - "desensitized" to the antibiotic and then the Syphyllis will be treated. The amounts will be slowly titrated until the pt can withstand a full course of treatment! (Well, I always thought it was cool, anyway. 😂🤷🏼♀️ Please correct me if I'm wrong, Mr. Microbiologist! 😊)
Most medications and antibiotics need a human body to work. It either works off the brain like opioid receptors, or triggers an immune response in the blood, just as vaccines basically give a copy of that virus but a more harmless version having the body attack it it’ll recognize the virus, triggering a immune response this is why sometimes you’ll get sick after getting a vaccine but for most of these medications to work it likely needs something in the body.
Did a lab in college about a year ago about the effects of using antibiotics on food grown for live stock. We took water samples of a farm runoff downstream and upstream. 75% of the bacteria downstream had penicillin resistance whilst something dumb like 90 upstream did not. Really showed me how humans just throwing a solution at a problem isn’t always the best choice
I have no clue what to say about this video, but I love the community you have in the comments. A lot of people know their stuff, they're having conversations, adding on information, it's really cool.
Penicillin works against the peptidoglycan cell wall in gram positive bacteria. Gram negative bacteria are generally not affected, as their peptidoglycan cell wall is protected by a lipidic membrane. This could explain why your bacteria have not been affected. By the way: mammals, including humans, do not have cell walls, their cells do not have peptidoglycan and are not affected by penicillin. Penicillin affects the good bacteria in the intestine, and this is the main reason why you may get side effects like diarrhea. Penicillin may damage the bacteria that contribute to digestion. I am not sure if simply dissolving a penicillin pill in water is enough.
Those are rod shaped bacilli and most of them are gram negative. Penicillin does not work as well for gram negative bacteria and not to mention resistance. There is a reason different antibiotics are used for different microbes,it's because many new penicillinase resistant varieties have emerged that are not affected by penicillin
Being a Biology student, you videos really help me to learn a lot about the theoretical knowledge in a practical way and to explore the wonders of Microbial world🤩! Just love it😊!
Penicillin exhibits time dependent killing time over MIC is key. Penicillinase is produced by many organisms. Not all bacteria have a cell wall. Penicillin only kills gram positive bacteria (ie those with a cell wall).
The freakiest thing I’ve ever seen under a microscope was a pinworm laying it’s eggs! That TOTALLY freaked me out! I watched it for about a half hour lay about 50 eggs! It’s birth canal seemed to move around inside the worm sucking up the eggs one by one like a vacuum cleaner and peristalsis them out. I was shocked! It was all so transparent!
You know that it has to metabolize in the human body to work right? You can't just crush stuff up and stick it on bacteria and expect it to work the same way that it does in the body. The body's got its own process to cut out the binders and everything else that's in the medication to get it to work like it's supposed to. The medication after it's metabolized works hand in hand with the body to destroy the bacteria.
The only reason we use antibiotic is that it didn't kill us. Not because it's the best at killing germ. If human can survive it. We will definitely use alcohol or formaldehyde for disinfection.
Penicillin inhibits the protein in bacteria that catalyzes the cross linking in the cell wall. It does this by irreversibly binding to the active site (a cystiene residue if I remember correctly). The penicillin resistance is caused by a similar protein to the target with a slightly more open active site that allows water in to break the bond between the penicillin and the protein. The protein goes back to being active and the active part of the penicillin is broken (a beta-lactam ring that gets broken during the binding process)
Most bacteria have Beta-lactamases that will hydrolyze the penicillin. This is a type of resistance which makes the drug pretty much useless in this day and age. Still available but mostly not used. Other Beta-lactam antibiotics are used over penicillin like cephalosporins (cefazolin) or mono-bactams (aztreonam) but they too have resistance issues. That's why we reserve the use of Vancomycin (a glycoprotein antibiotic that binds to D-Ala-D-Ala of the peptidoglycan preventing PBP [the enzyme that crosslinks the peptidoglycan synthesizing a functioning cell wall]). Vancomycin (and others) is/are used when Beta-lactam antibiotics are found ineffective. Vancomycin is another antibiotic that has resistance issues too (just less). Please take your antibiotics responsibly, take them as prescribed and finish them.
@@justmadz8447 um...no...if it were a resistance issue, some/most things would still die. The penicillin must've degraded and not been effective anymore, hence an example of **shelf life**
Many antibiotics are currently less effective, not only because of over‐prescription for minor complaints, but (both legally and illegally) they're too often used in our meat and poultry food chains.
Now you should thank Iranian and great man of the old century Khawarazmi Discoverr Alcohol, the world calls him the Modern fatherMeidicin without any noble prize when Jew zionist einstein had a Nobel when Albert Einstein had a terrible reputation as a career plagiarist. It was well known among his colleagues that he had plagiarized the special theory of relativity from Hendrik Antoon Lorentz and Jules Henri Poincaré.
What happens in the person who is allergic to penicillin? Why does it attack our systems to a point it causes swollen eyes,lips and ungodly high fevers for days ? What is that drug doing then? And why can a person take the pill form without reaction,but not with 10 cc injection?❤
@@ugaladh So my system is causing the fevers and swellings. Why does my system react as it does,able to take the pill form but not the 10 cc of shot❓ Is the liquid the penicillin is mixed with the culprit?
Penicillin are Beta Lactams as they have a Beta Lactam Ring Which gets Absorbed and Stops the Bacteria For Forming the New Cell Wall. So there are 2 Options Now If the Given Set of Bacteria have Beta Lactamase Gene which Digests and Compete We Add additional Med called Beta Lactamase i (inhibitor) for example the Clauvanate in Amox+clauv This Inhibitor damages those Enzymes. More Over during a cell Cycle the Formation of the Cell wall happens After cytokinesis or after division. So it may take Upto 30 min. -Doc Here.
No more plain penicillin is in use much, they got clavulanic acid for wall lysis ....and that made penicillin /amoxicilin a broad spectrum antibiotics ...work against most of infections conditions
Clavulanic acid itself doesn't have any antibiotic properties. What it is is a beta-lactamase inhibitor, so it destroys an enzyme some penicillin-resistant bacteria use to inactivate beta-lactamic antibiotics (which includes penicillin and amoxicillin). Also, although it improves coverage, it's still not a broad-spectrum antibiotics. A broad-spectrum antibiotic covers both gram positive and negative bacteria. Penicillin and its derivates generally cover only gram negatives.
if you were in my head right now, all you'd see is mental images of me chugging all of those solutions followed by instantly recoiling at the thought, but played on repeat nonstop.
@@larswilms8275well assuming he consumed enough alcohol to actually kill all bacteria in his body, the alcohol would do the job first. It's said that there's no coming back after .30 BAC. Which even that isn't enough to kill bacteria.
I find it pretty funny you expected a different result. You tried to test the efficacy of a compound that's infamous for depending on a highly pH dependant mechanism...without removing ANY of the various binders, colorants, fillers or excipients. Did you even know the initial pH of your solvent? How about the base pH of the specimen you're trying to lyse. Btw, if you're wanting to induce lysis, you'll need to have a target MBC...but for this experiment, none of that ever mattered to begin with here, thanks to something called biofilm mediated resistance. You just tested an expired sample of a drug, in the least effective way, on exactly the kind of organisms that are known for being highly resistant to said drug. Keep making videos though you make good content, including this happy accident! Antibiotic resistance, especially biofilm mediated and multidrug resistance should have people concerned WAY MORE than it seems they are!
Wait! This is not the way to know how effective an antibiotic is. You need blood agar in petry disc that has spread specific germ (after through several treatment) than put a tablet of the antibiotic on the blood agar. Close the disc and wrap it with paper than put it in a warm cabinet (37°C) for 1 - 2 days. After that you can see a free germ ring around the antibiotic. More bigger the ring is more stronger the antibiotics is
Do food service sanitizer. It's like a mysterious red liquid and smells so good. Ik it's available from sam's club and i imagine most other big box stores have it.
It’s them phenols! The original red stuff was literally phenol, then they switched to creosol (both made from wood tar/coal). The original Lysol was such a carboxylic soap product, and in parts of the world they still use it. The more modern food service phenols have more complicated side chains so they smell less… tar-ish. You probably have chloroxylenol in your hand wash.
Oh wait, it’s sanitizer, not hand wash… Right. Sani-quad is probably the thing you’re talking about, and it uses quaternary ammonium to kill stuff. The quat active ingredient actually doesn’t smell like anything and they just add whatever fragrance. Bit less fun I know.
Get a blood test done for mold hypersensitivities. 25% of Northern European descents cannot process most molds and fungus And symptoms of Toxic Mold Syndrome are never identified by western medicine and symptoms are very strange and vary greatly from person to person. For example… I have symptomatic narcolepsy and can tell in 30 minutes if there is mold in a building and if I stay much longer I start passing out and look like I’m on drugs. My sister on the other hand, after I discovered this issue on my own after requesting mold blood tests be done and results amazed me-she became celiac after staying in moldy dorms at MIT (my symptoms onset after staying in moldy dorms at ASU) and extremely sensitive to any gluten or yeast like her throat will get all fuzzy and close up just from touching it! Moral of story-if having weird health issues with no progress or explanation and you are a Northern European descent… request a mold panel hypersensitivity blood test from you primary care doctor (: can’t hurt right? Besides the blood draw part… lol
Damn that’s crazy just like liver failure, also crazy like alcohol poisoning, also crazy like increased chance of cancer, super crazy like weight gain!
@@griffinhigh6646 Hello there my furry friend, I couldn't help but to notice you misunderstood "whiskey drinkers" as "alcoholic, binge drinking maniacs" when formulating your reply! As with any substance, a healthy relationship and understanding of what we put into our bodies and enjoyment in moderation are key. A person who has whiskey a few times a week will be much healthier than a person who has pizza and burgers for every meal.
Try dissolving it in apple cider vinegar next time first before adding it to the bacteria. Also do a control sample with only apple cider vinegar added to the bacteria.
"superbug is like a truck And penicillin is a duck That's sitting on the road for luck..." "Unnecessary anti-b's Likely killed humanity"-Superbug, by King Gizzard and the Lizard Wizard
Im no expert (yet) but 2 very simple things: 1. Old medicine loses it's efficiency with time, specially if it's not well preserved (paracetamol, aspirin, pretty much anything gets ruined in hot places. 2. Medicine (compressed tablets specifically) are composed of Active principle (the med) and excipient (filling, protects the actual medicine and helps so it disolves where it's suposes to solve) You can separate it with a liquid-liquid method, tho since penicillin don't just kill bacteria, it won't be any different... but it'll be pure and free of excipients for experiments :D (not consumption)
Truly comforting to see the rubbing alcohol absolutely nuke them. Every time I get a bad cut I immediately douse it in isopropyl and wrap it tight. Pretty much never use neosporin or anything.
Why continue to expose your body for 10-14 days if 5-7 days cures? Perhaps that feeds in to ATB resistance, as well. Got my Dee Arr Period in this crap.
*Penicillin* : Here i come Folks. (Poured itself to the mass of bacterias) *Bacterias* : Ah... the good ol' penicillin, i Feel good 🕺🏼🕺🏼🎤🎶 *Pure Alcohol* : My turn now!! *Bacterias* : Aaaaaaaaahhhhhhhhhh.
![[4K] Watch SpaceX Catch A Starship Rocket From Space!!! #IFT5](http://i.ytimg.com/vi/pIKI7y3DTXk/mqdefault.jpg)
*adds penicillin*
bacteria: "this shi weak af."
*adds alcohol*
bacteria: 💀💀💀
😅😅😅
Lol sounds about right
Isopropyl alcohol is just napalm for bacteria
Its super easy to kill bacteria and viruses. The tricky part is killing them without killing the host.
🤨
The message here is always drink isopropyl alcohol instead of taking antibiotics. Thank you Doctor for clearing this up 👍🏻.
LMAO
Yeah, meeting the Lord is better than staying here!! 😂😂😂
lol
Lmfao
To be safe you should also apply it to your entire body's surface.
The lesson here is: stop using pills, just drink alcohol.
Traveling at over 100mph (preferably by car) decreases the amount of bacteria when paired with alcohol.
@@aspektric I don't have a car, will I achieve the same effect running at 100mph?
That’s why Russians are so healthy… all the vodka!!
Does 80 proof Vodka with a dash of Kahlua have enough alcohol to do the trick?
rubbing alcohol is 70% isopropyl alcohol denatured , it will not make you drunk but it very well may kill you regardless by destroying cells
Got it; drink alcohol when you’re sick.
😂
I came here to mention that. Thanks!
Make sure it's the pure stuff to be most effective 😊
Drink away, champ.
Alcoholist are the most cleanest man walk on earth
Two things here:
1. Simply dissolving pencillin won't release the active form. Since it's an oral formulation, it must go through stomach to become active. So, a solution of penicillin won't be effective against bacteria
2. None of our cell has cell wall. Bacterial cell wall has peptidoglycan. Penicillin disrupts synthesis of this chemical. Now fungal cell wall lacks peptidoglycan hence antibiotics aren't very effective against them
Penicillin VK isn't a prodrug, it doesn't need to be activated by first pass metabolism and specifically PVK is significantly inactivated in first pass. It does need the low pH of the GI tract to be properly absorbed but that's not "activation" per se.
the penicilin he used is old prolly expired that explains why it didnt work
To the thing no.1 into whose stomach did Alexander Fleming put the penicillin to make it active? 😄😂🤣🤣🤣🤣🤣
So dissolving it in acid would make it work proper in theory
Man, just imagine getting infected by fungus 💀
And that’s why you have to take a FULL COURSE of antibiotics and not just stop after a few days when your feeling better. It’s to make sure the bacteria is aniahlated or you risk antibiotic resistant bacteria.
You do have to take the full course but that's not why it didn't work in the video.
There are two major families of antibiotics: *bacteriocidal* that kill bacteria and *bacteriostatic* that prevent them from multiplying and let your own T-cells do the rest. Penicillin is bacteriostatic.
By the way, although the mechanism is called "bacterio"-cidal/static, most antibiotics work on *all* cells.. The bacteria will stop multiplying, but so will your T-cells. That's why it is important to start taking antibiotics only after the symptoms have fully developed, indicating that your body has produced enough T-cells to fight the infection.
that is why you get your immune system stronger so you dont need antibiotics. lol
No that's not how resistance works. If any resistant bacteria are present they will still be around regardless of whether or not you finish the full course. Stopping it early doesn't magically create resistant bacteria, it just may not kill all the bacteria.
It's actually the opposite of what you are saying. If someone is one antibiotics for a long time there is constant selective pressure in favor of producing resistant bacteria.
Some people just take one shot pf tylenol and stop as soon as they feel better 👀
@@benzun9600 drink from the garden hose to boost that immune system, my boi. Tried and true method
The penicillin in pills is not per say penicillin. It’s an analogue that needs to pass through the stomach acids and then be activated by cytochromes in the liver to be active
Thank you
Thank you.. the 1st person to get it right
Per se* not "per say." It's Latin.
Wow... reading your response makes me feel completely retarded. Full blown retarded. Ill just trust the dude at Walgreens. Not the one who greets me at the counter, but the guy in the white coat way back there, the guy who always tells me that i should ask my doctor for whatever advice i ask him.
It takes 18 to 24 hours for penicillin V to be at 10% it takes three to five days to be at 100% effective... that's why when they do a rapid antibiotic test at the emergency room or doctor's office it can take up to 24 hours to get your results... that's why most doctors will prescribe antibiotics by symptoms and they usually prescribe a spectrum antibiotic... amoxicillin.
doxycycline.
cephalexin.
ciprofloxacin.
clindamycin.
metronidazole.
azithromycin.
sulfamethoxazole and trimethoprim..
Its not ineffective, its just that Penicillin only acts with bacterias sensitive to it. Thats why doctors prescribe certain antiobiotics depends on a specific desease.
Lies again? Mola Sport Melbourne Sydney
"I felt a great disturbance in the Force, as if millions of voices suddenly cried out in terror and were suddenly silenced."
Home go Yoda. Drunk you are
Underrated comment...
Me after I wash my hands
and after, sternly mutter, "I am your God"
I would like to see you treat mouth germs with salt water. Grew up with mother who was always urging gargling with salt water for many problems.
Gargling salt water gets a lot of its beneficial effect from the way strong salt water will draw water from inside the infected area through ionic osmosis, helping to mechanically flush the bacteria and dead cells outward into your mouth where you spit it out after the gargle.
he did do a vid of that
@@marjieestivill imagine getting dragged out of ur house while ur whole family exploding from too much fluid lol
You don't really want to kill your mouth germs. You just want to break up the colonized ones, the plaque.
Yes! My mom too. Everything from doing it every so often just because to tooth problems to sore throats and ulcers. She was right most of the time. I'd still love to see it in action though and hear scientifically why it works or doesn't work.
Day 6 of asking to show sperm under the microscope?
but why? thats kinda boring
Ovum as well then? Please?
@@udayvarma756 Unless he's got access to anaesthetic and needles, that's gonna be like looking for a needle in a haystack.
Not to mention he'd have a hard time getting a woman to collect all her period blood for a video.
One question. Whose?
Ya could look at tadpole videos to hold ya over until he gets around to it...
😁
Every bacteria is gangster until the isopropyl alcohol appears
😅❤😅❤
Nice way to learn how medicine works.
Would be interested in seeing what a sneeze/mucus looks like, and maybe add some sort of disinfectant (bleach/lysol/peroxide, etc)
Would be cool 🤔
He has a video with a sample of something from his throat. It had many different bacteria and body cells (like mucus and white blood cells). You can find it if you scroll through his page :)
Quick edit: it’s a sample from coughing I believe
Or a sneeze from someone w/ a virus vs. allergies vs. bacterial infection
You can't see a virus with a light microscope
@@rorichacon6094he'd need an electronic microscope to see a virus. Would be cool but I doubt he has such access to one
The takeaway:
Don't drink dirty pond water
And
Don't follow it up with expired penicillin and expect that to treat your dysentery.
@Walter “The Cat Yeeter” B it's a biodrug, they're sensitive to environmental degradation over time. If it's expired it's no longer capable of doing the job. Sulfonamides are simpler, much more shelf stable antibiotics, and take far longer to degrade in storage.
No no no, the takeaway is:
IF you happen to drink dirty pond water, just drink bleach afterwards!!
Pen V may not be effective on this rod or cocci....it's how meds work over time it's not cidal agent like alcohol is.
@@AlexanderTzalumen no actual correct time means loss in potency so 2 tab may be effective as 1 old one..my educated opinion.
@@lemmon-up4er that assumes penicillin has a half life decay, rather than simultaneous decay. I don't think that's a safe assumption to make.
CORRECTION: it only affects the BACTERIAL cell wall, and only when the bacteria is growing
It prevents bacteria from reproducing. This lets the immune system come in and fight the infection.
@@pcproffy No, no, no. It not only prevents them from reproducing, it prevents them from GROWING, as it prevents new cross-links in peptidoglycan / murein, out of which the bacterial cell wall is made. if the cell grows, the cross-links are broken, so the cell wall can expand by integrating new chains of peptidoglycan. but due to penicillin buinding to peptidoglycan, new cross-links between those new and old chains can't be built, so the cell just leaks out. same happens during reproduction, as the cell wall devides during mitosis.
Plus it is a time dependent antibiotic, meaning that it is only effective if the bacteria is exposed to a sufficient concentration of the medication for an extended period of time
Bleach basically has the ability to kill most bacteria.
A bacterium must keep its cell wall intact at all times
The penicillin would have a lethal affect on me
Same 😭 idk if ur allergic but I am
@JohnAustin56 - the *Effect* might be lethal, giving you the *Affect* of death. Anaphalaxis is nothing to joke about, but using the wrong word _is._
Same 😅
You.might be a bacteria
My good sir, is your middle name Staphylococcus Aureus, by any chance?
Without a control of an unexpired penicillin test, there's no conclusion here.
There is a conclusion
Penicillin doesnt kill bacteria at all
aso the entire experiment is false
Poster = rekt
You don't need a control, it was never going to work
Penicillin: haha this shit weak as fu-
Alcohol: *30 kill streak tac nuke*
It didn’t kill them, but it didn’t seem like they were having a good of a time either. It was a party before that happened😂
it was an entire universe to them
You need only take a college course of microbiology to learn about different bacteria and different antibiotics. This is why you are prescribed certain antibiotics depending on your symptoms and also why it is so important to be honest with your Dr about that because different antibiotics work depending on what bacteria you are infected with. That's also why there are "broad spectrum" antibiotics because they will kill any and all bacteria including the natural flora, or natural bacteria that your body creates.
I once had a doctor that did blood samples in his office. Ten minutes later he prescribed exactly what you needed!!! That was 30 yrs ago! Now you have to run all over town to get blood drawn but have already been given drugs
@@danielking221what exactly did he said it was?
Not even. They taught that in bio my freshmen year.
Is there anything that will kill strep in the body? I had strep throat years ago and traces still show up in tests
"you *only* need to take a college course of microbiology to..."
Yeah i have the sudden urge to learn about Microbes so Lemme reach in my pocket, drop $7000 on a semester of school just so i can scratch my intellectual curiosity
RUclips is better my guy
Well here is the deal. Penicillin only works on very specific bacteria. You appear to have rod shaped bacteria which is often something like Bacillus that are in spore form. In addition, penicillin also degrades in efficacy over time. But its doubtful it worked because you gave it to spore formers.
I am a microbiologist.
The last picture looks exactly like mushroom mycelium when it grows. Any comment on what that was ?
kinda works on unaliving a father figure
fyi, my dad is allergic to penicillin. to the point it will kill him. like, tf
I actually wondered about that - i noticed the small rods - but dismissed my mediocre microbiology education because I'm only an RN and not a Microbiologist.
Yagamil46:
(And to anyone else with an allergy to Penicillin)
Fun Fact: So the only thing to kill Syphilis is Penicillin.
What to do when a pt has Syphyllis but cannot take Penicillin?
Pt will be admitted indefinitely wifh a constant IV drip of VERY SMALL AMOUNTS of Penicillin and Epinephrine and fluids until the pt has become - for lack of a better word /explanation - "desensitized" to the antibiotic and then the Syphyllis will be treated.
The amounts will be slowly titrated until the pt can withstand a full course of treatment!
(Well, I always thought it was cool, anyway. 😂🤷🏼♀️ Please correct me if I'm wrong, Mr. Microbiologist! 😊)
Most medications and antibiotics need a human body to work. It either works off the brain like opioid receptors, or triggers an immune response in the blood, just as vaccines basically give a copy of that virus but a more harmless version having the body attack it it’ll recognize the virus, triggering a immune response this is why sometimes you’ll get sick after getting a vaccine but for most of these medications to work it likely needs something in the body.
Did a lab in college about a year ago about the effects of using antibiotics on food grown for live stock. We took water samples of a farm runoff downstream and upstream. 75% of the bacteria downstream had penicillin resistance whilst something dumb like 90 upstream did not. Really showed me how humans just throwing a solution at a problem isn’t always the best choice
that's amazing
Great info-was curious about those AB effects myself!
I have no clue what to say about this video, but I love the community you have in the comments.
A lot of people know their stuff, they're having conversations, adding on information, it's really cool.
YES
Thanks dude. I actually learned something.
Please, tell us what you learned...?
Isn't that cool that there's still folks out here that have actual useful knowledge? Incredible!
Penicillin works against the peptidoglycan cell wall in gram positive bacteria. Gram negative bacteria are generally not affected, as their peptidoglycan cell wall is protected by a lipidic membrane. This could explain why your bacteria have not been affected.
By the way: mammals, including humans, do not have cell walls, their cells do not have peptidoglycan and are not affected by penicillin. Penicillin affects the good bacteria in the intestine, and this is the main reason why you may get side effects like diarrhea. Penicillin may damage the bacteria that contribute to digestion.
I am not sure if simply dissolving a penicillin pill in water is enough.
I believe it was also an old pill, prob expired. Would that also affect the likelihood of killing more bacteria?
Those are rod shaped bacilli and most of them are gram negative. Penicillin does not work as well for gram negative bacteria and not to mention resistance. There is a reason different antibiotics are used for different microbes,it's because many new penicillinase resistant varieties have emerged that are not affected by penicillin
I love this guy, because he sounds like he's never been excited about anything in his entire life
Being a Biology student, you videos really help me to learn a lot about the theoretical knowledge in a practical way and to explore the wonders of Microbial world🤩! Just love it😊!
Look at the top comment he knows a lot more then the guy in the video
Try to absorb it in an acid since, the pill is designed to be absorbed via stomach acid
Penicillin exhibits time dependent killing time over MIC is key. Penicillinase is produced by many organisms. Not all bacteria have a cell wall. Penicillin only kills gram positive bacteria (ie those with a cell wall).
Made in China?
The freakiest thing I’ve ever seen under a microscope was a pinworm laying it’s eggs! That TOTALLY freaked me out! I watched it for about a half hour lay about 50 eggs! It’s birth canal seemed to move around inside the worm sucking up the eggs one by one like a vacuum cleaner and peristalsis them out. I was shocked! It was all so transparent!
That sounds fucking gross
I thought Patrick Bateman was teaching me science for a second
Maybe that's because penicillin is bacteriostatic and not bacteriocidal.
Because there are different type bacterias
You know that it has to metabolize in the human body to work right? You can't just crush stuff up and stick it on bacteria and expect it to work the same way that it does in the body. The body's got its own process to cut out the binders and everything else that's in the medication to get it to work like it's supposed to. The medication after it's metabolized works hand in hand with the body to destroy the bacteria.
Thank you, I was about to be overcome by all the ignorance
@@duvoncorbitt947 what? What are you even talking about
Bro found a whole other world under that microscope 💀
So you're saying I should inject rubbing alcohol straight into my veins? Noted.
They actually have administered Hydrogen Peroxide by IV to treat cancer. Its not rubbing alcohol but js...
I got needles
I heard it was an Executive Order...
I read that as “Old penicillin vs Germans” wondering if they had a different reaction to it 😂
Remember kids. Alcohol is more effective then pills
Well, until the Molly kicks in
The only reason we use antibiotic is that it didn't kill us.
Not because it's the best at killing germ.
If human can survive it.
We will definitely use alcohol or formaldehyde for disinfection.
Penicillin inhibits the protein in bacteria that catalyzes the cross linking in the cell wall. It does this by irreversibly binding to the active site (a cystiene residue if I remember correctly). The penicillin resistance is caused by a similar protein to the target with a slightly more open active site that allows water in to break the bond between the penicillin and the protein. The protein goes back to being active and the active part of the penicillin is broken (a beta-lactam ring that gets broken during the binding process)
how was this discovered
Thanks for the information. Was this observed? Or inferred from observation?
Beta lactumase are much better.
This was good info but a poor example.
So when I'm sick I should just drink rubbing alcohol. Thanks for the tip.
Is it possible to make bacteria fight each other under the microscope?
🤣
Maaaan when he say “it’s grown a thick bio film” it hit different
Most bacteria have Beta-lactamases that will hydrolyze the penicillin. This is a type of resistance which makes the drug pretty much useless in this day and age. Still available but mostly not used. Other Beta-lactam antibiotics are used over penicillin like cephalosporins (cefazolin) or mono-bactams (aztreonam) but they too have resistance issues. That's why we reserve the use of Vancomycin (a glycoprotein antibiotic that binds to D-Ala-D-Ala of the peptidoglycan preventing PBP [the enzyme that crosslinks the peptidoglycan synthesizing a functioning cell wall]). Vancomycin (and others) is/are used when Beta-lactam antibiotics are found ineffective. Vancomycin is another antibiotic that has resistance issues too (just less).
Please take your antibiotics responsibly, take them as prescribed and finish them.
Well explained, this is what they taught us in med school!
@@smellypatel5272 In pharmacy school myself. Haha
A fine example of shelf life.
Adaptation and drug misuse*
@@justmadz8447 um...no...if it were a resistance issue, some/most things would still die. The penicillin must've degraded and not been effective anymore, hence an example of **shelf life**
No antibiotics dont work without a immune system
@@mx208 bingo.
@@robbobthecorncobjriii8195 shelf life deez nuts
Bro that alcohol was like the apocalypse
This is the best 'finish your antibiotics' commercial that I've ever seen 😂
Conclusion. A shot of vodka will work better than penicillin to kill a cold.
Reason for it not working is because people seem to use it for a simple cut. So the bacteria has evolved to resist the medication.
It's also entirely possible it's all gram-negative bacteria, which do not have cell walls and aren't affected by penicillin.
Many antibiotics are currently less effective, not only because of over‐prescription for minor complaints, but (both legally and illegally) they're too often used in our meat and poultry food chains.
Woah putting that alcohol in had like an atomic bomb reaction, everything just stopped dead in it’s track with no sign of life, amazing!
Now you should thank Iranian and great man of the old century Khawarazmi Discoverr Alcohol, the world calls him the Modern fatherMeidicin without any noble prize when Jew zionist einstein had a Nobel when Albert Einstein had a terrible reputation as a career plagiarist. It was well known among his colleagues that he had plagiarized the special theory of relativity from Hendrik Antoon Lorentz and Jules Henri Poincaré.
It stops the cells from dividing. Penicilline (B-lactam) generally doesn't kill bacteria.
What I’d like to see is how the viewers knew about an old penicillin you had lying around there?
hey yeah waitaminnit
Because he knows everyone else personally and they visit him often🤡
Love your channel I was wondering if you could do one of denture cleaner 👍
Penicillin is an antibiotic not a bactericide. If this logic worked shotting ethanol would kill germs. This generation makes me cry.
Didn't think the Effect would be different outside the body. Learn something new all the time!
God Bless
I am allergic to penicillin. I almost died when it was first used on me.
What happens in the person who is allergic to penicillin? Why does it attack our systems to a point it causes swollen eyes,lips and ungodly high fevers for days ? What is that drug doing then? And why can a person take the pill form without reaction,but not with 10 cc injection?❤
The Penicillin isn't attacking your cells or systems - it is your own immune system causing this.
@@ugaladh So my system is causing the fevers and swellings. Why does my system react as it does,able to take the pill form but not the 10 cc of shot❓ Is the liquid the penicillin is mixed with the culprit?
im allergic to it as well, but not sure how I am its unknown to me and my family but its on my record
So alcohol is better than penicillin. I see...I quit drinking like a fool then
Gotta drink 70% alcohol though my man. 🍸🥂🥂🍾
Nicks strength and power microscope brother right here.
I honestly want to see what Fresh milk out the cow looks like
Penicillin are Beta Lactams as they have a Beta Lactam Ring Which gets Absorbed and Stops the Bacteria For Forming the New Cell Wall.
So there are 2 Options Now
If the Given Set of Bacteria have Beta Lactamase Gene which Digests and Compete
We Add additional Med called Beta Lactamase i (inhibitor) for example the Clauvanate in Amox+clauv
This Inhibitor damages those Enzymes.
More Over during a cell Cycle the Formation of the Cell wall happens After cytokinesis or after division.
So it may take Upto 30 min.
-Doc Here.
Are they competitive or noncompetitive inhibitors. Possible MCAT question
No more plain penicillin is in use much, they got clavulanic acid for wall lysis ....and that made penicillin /amoxicilin a broad spectrum antibiotics ...work against most of infections conditions
Clavulanic acid itself doesn't have any antibiotic properties. What it is is a beta-lactamase inhibitor, so it destroys an enzyme some penicillin-resistant bacteria use to inactivate beta-lactamic antibiotics (which includes penicillin and amoxicillin).
Also, although it improves coverage, it's still not a broad-spectrum antibiotics. A broad-spectrum antibiotic covers both gram positive and negative bacteria. Penicillin and its derivates generally cover only gram negatives.
if you were in my head right now, all you'd see is mental images of me chugging all of those solutions followed by instantly recoiling at the thought, but played on repeat nonstop.
Bacteria: You can't defeat me.
Penicillin: I know, but he can.
Alcohol:Enters the chat.
As an alcoholic i feel safe having no bacteria in me
I wonder what would kill you first. The lack of bacteria or the alcohol?
I am leaning towards lack of bacteria, tbh.
Cirrhosis incoming
@@larswilms8275well assuming he consumed enough alcohol to actually kill all bacteria in his body, the alcohol would do the job first.
It's said that there's no coming back after .30 BAC. Which even that isn't enough to kill bacteria.
Your liver will be the problem.
It needs to pass thru the digestive system. Liquid antibiotics for IV use would be observable at work on that slide.
Try doing a culture and sensitivity with different types (classes) of antibiotics!
Great video!
I find it pretty funny you expected a different result. You tried to test the efficacy of a compound that's infamous for depending on a highly pH dependant mechanism...without removing ANY of the various binders, colorants, fillers or excipients. Did you even know the initial pH of your solvent? How about the base pH of the specimen you're trying to lyse. Btw, if you're wanting to induce lysis, you'll need to have a target MBC...but for this experiment, none of that ever mattered to begin with here, thanks to something called biofilm mediated resistance. You just tested an expired sample of a drug, in the least effective way, on exactly the kind of organisms that are known for being highly resistant to said drug.
Keep making videos though you make good content, including this happy accident! Antibiotic resistance, especially biofilm mediated and multidrug resistance should have people concerned WAY MORE than it seems they are!
Instructions unclear, I drink a gallon of rubbing alcohol and now I can breath sound
There's always that 0.1%
Your squirrel friend's got your back . 😄
I would like to see dog saliva compared to human saliva under the microscope
Wait! This is not the way to know how effective an antibiotic is. You need blood agar in petry disc that has spread specific germ (after through several treatment) than put a tablet of the antibiotic on the blood agar. Close the disc and wrap it with paper than put it in a warm cabinet (37°C) for 1 - 2 days. After that you can see a free germ ring around the antibiotic. More bigger the ring is more stronger the antibiotics is
This is what they taught me in the Medical Lab course,
Paper Cups - Always curious if any chemical residues left inside the cup by the manufacturer ..
Thank you!
You gave me a new paranoia
The logic behind toads is that they survived in mud so they must have something to help fight bacterial.
Do food service sanitizer. It's like a mysterious red liquid and smells so good. Ik it's available from sam's club and i imagine most other big box stores have it.
It’s them phenols! The original red stuff was literally phenol, then they switched to creosol (both made from wood tar/coal). The original Lysol was such a carboxylic soap product, and in parts of the world they still use it.
The more modern food service phenols have more complicated side chains so they smell less… tar-ish. You probably have chloroxylenol in your hand wash.
Oh wait, it’s sanitizer, not hand wash… Right. Sani-quad is probably the thing you’re talking about, and it uses quaternary ammonium to kill stuff. The quat active ingredient actually doesn’t smell like anything and they just add whatever fragrance. Bit less fun I know.
Multi quat. Quaternary ammonium.
Food service sanitizer. Is highly toxic to cats and dogs. Full strength causes chemical burn to flesh.
What is it for? Do you sanitize the food with it???
@@orchdork775 Best with vodka!
Do it for blood of a person who's allergic. I had an allergic reaction to penicillin (we didn't know i was allergic) so i want to know what happens.
Same here
Get a blood test done for mold hypersensitivities.
25% of Northern European descents cannot process most molds and fungus
And symptoms of Toxic Mold Syndrome are never identified by western medicine and symptoms are very strange and vary greatly from person to person.
For example… I have symptomatic narcolepsy and can tell in 30 minutes if there is mold in a building and if I stay much longer I start passing out and look like I’m on drugs.
My sister on the other hand, after I discovered this issue on my own after requesting mold blood tests be done and results amazed me-she became celiac after staying in moldy dorms at MIT (my symptoms onset after staying in moldy dorms at ASU) and extremely sensitive to any gluten or yeast like her throat will get all fuzzy and close up just from touching it!
Moral of story-if having weird health issues with no progress or explanation and you are a Northern European descent… request a mold panel hypersensitivity blood test from you primary care doctor (: can’t hurt right? Besides the blood draw part… lol
That's why whisky drinkers stay healthy 😊
Damn that’s crazy just like liver failure, also crazy like alcohol poisoning, also crazy like increased chance of cancer, super crazy like weight gain!
@@griffinhigh6646 Hello there my furry friend, I couldn't help but to notice you misunderstood "whiskey drinkers" as "alcoholic, binge drinking maniacs" when formulating your reply!
As with any substance, a healthy relationship and understanding of what we put into our bodies and enjoyment in moderation are key. A person who has whiskey a few times a week will be much healthier than a person who has pizza and burgers for every meal.
Ahh yes alcoholics
No they dont 😂
What about wine 😂
Try dissolving it in apple cider vinegar next time first before adding it to the bacteria. Also do a control sample with only apple cider vinegar added to the bacteria.
Penicillin has a shelf-life you know
😅❤😅❤
It’s probably not the correct antibiotic for what bacteria you have here
Or it's on a prodrug form that needs to be activated by acid or enzymes in the body
@@Martin1jg Penicillin Vk is not a prodrug...
Does the penicillin have an expiration date?
I’ve been reading a lot on cilantro oil and it’s affects as antibiotic. Might be worth a video. Thanks for the content dude
Instructions unclear, Started drinking alcohol than taking medicine
Could you pls show us your blood cells, then drop bad bacteria. Im curious how those bacteria assimilate/highjack our cells
How about doing some garlic against bacteria
"superbug is like a truck
And penicillin is a duck
That's sitting on the road for luck..."
"Unnecessary anti-b's
Likely killed humanity"-Superbug, by King Gizzard and the Lizard Wizard
Penicillin: Yeah… that don’t work at all…
Alcohol: *immediate flashbacks of Osmosis Jones*
Penicillin also only works on gram-positive bacteria, so some of them might not have been gram-positive
Another reason to enjoy bourbon
You should ALWAYS take all your antibiotics when they're prescribed. You shouldn't have any leftovers.
Im no expert (yet) but 2 very simple things:
1. Old medicine loses it's efficiency with time, specially if it's not well preserved (paracetamol, aspirin, pretty much anything gets ruined in hot places.
2. Medicine (compressed tablets specifically) are composed of Active principle (the med) and excipient (filling, protects the actual medicine and helps so it disolves where it's suposes to solve)
You can separate it with a liquid-liquid method, tho since penicillin don't just kill bacteria, it won't be any different... but it'll be pure and free of excipients for experiments :D (not consumption)
Truly comforting to see the rubbing alcohol absolutely nuke them. Every time I get a bad cut I immediately douse it in isopropyl and wrap it tight. Pretty much never use neosporin or anything.
How bout blackhead and face pore under microscope?
My question is why didn't you finish your course of antibiotics?!
Why continue to expose your body for 10-14 days if 5-7 days cures?
Perhaps that feeds in to ATB resistance, as well.
Got my Dee Arr Period in this crap.
Everybody gangsta till the alcohol steps in
Bacteria is harmed while making this video
💥 Colloidal Silver 💥
yea good kall lets see tht my guy!!!
Fell bad for the little germs : (
*Penicillin* : Here i come Folks.
(Poured itself to the mass of bacterias)
*Bacterias* : Ah... the good ol' penicillin, i Feel good 🕺🏼🕺🏼🎤🎶
*Pure Alcohol* : My turn now!!
*Bacterias* : Aaaaaaaaahhhhhhhhhh.
Try Raw Garlic against Bacteria ?
Yes but as long as he waits 10 minutes for it to create allicin
@@czed7515 It takes 10 minutes to do that ?
@@jayhockley8841 yeah you have the mince it or mash it and it will make the alicium. I take it every night. Never get sick.
@@czed7515 👍 Yeah me too .
I cut up 2 cloves and drink with water every morning , 1st thing .
Germs don’t like acid (most of the time)
Can you try it with oregano-rosin? Some test say its better against bacteria than penicillin, i would love to see it in action