Immunocytochemistry (ICC) - Video Protocol Series

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Immunocytochemistry (ICC) - Video Protocol: • Immunocytochemistry (I...
Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen.

[@smartcookie823]

Thank you! so helpful

[@mohitjesani2797]

what's the purpose of demineralised water?
mounting chemical used?