CryoSPARC 3D Flex Custom Mesh Generation

In this video tutorial we provide a detailed explanation 3D Flex Custom mesh generation. We begin by explaining when your data might benefit from a custom mesh, then move on to the actual process of mesh creation. Finally, we discuss some important considerations when deciding on a mesh topology, especially for membrane proteins.
This video accompanies the written discussion of the same topics and uses data from EMPIAR 10261, originally published by Xu and colleagues.
CryoSPARC Guide link: guide.cryospar...
EMPIAR 10261: www.ebi.ac.uk/...
Chapters
00:00 Introduction
01:01 Part One - What is the goal of 3D Flexible Refinement, and why do we need a mesh
06:32 Part Two - What jobs are required before beginning
10:44 Part Three - Making the mesh
18:27 Part Four - Launching the job
26:08 Part Five - Mesh job results
31:14 Part Six - Mesh fusion and topology