Видео 19
Просмотров 107 193

CryoSPARC Live™: The advantages of on-the-fly processing in cryo-EM

45:11

CryoSPARC 3D Flex Custom Mesh Generation

46:01

Mask Creation in ChimeraX for Cryo-EM Processing

21:22

CryoSPARC v4.4: Workflows

13:23

CryoSPARC v4.4: Reference Based Motion Correction

17:22

CryoSPARC 3D Flexible Refinement Tutorial

1:22:11

Tree View and Job Groups in CryoSPARC v4.6

In CryoSPARC v4.6, we introduce rebuilt tree, card and table views that are optimized for performance to support large workspaces and projects. The new targeting system allows you to quickly find and navigate to any job in a view. We also introduce the Job Groups feature which allows you to collapse multiple jobs into a single card to maintain the legibility of a workspace. Learn more: guide.cryosparc.com/application-guide-v4.0+/job-views-cards-tree-and-table

Видео

CryoSPARC Live™: The advantages of on-the-fly processing in cryo-EM

45:11

CryoSPARC Live™: The advantages of on-the-fly processing in cryo-EM

Просмотров 2743 месяца назад

Modern cryo-EM datasets are typically several terabytes in size and often take over a day to collect. With traditional batch-based processing, users must wait for all files to be available on their local machine before beginning to process them. Moreover, processing must be performed entirely in serial, forcing later steps to wait until earlier steps (like motion correction) have completed. Thi...

CryoSPARC 3D Flex Custom Mesh Generation

46:01

CryoSPARC 3D Flex Custom Mesh Generation

Просмотров 7346 месяцев назад

In this video tutorial we provide a detailed explanation 3D Flex Custom mesh generation. We begin by explaining when your data might benefit from a custom mesh, then move on to the actual process of mesh creation. Finally, we discuss some important considerations when deciding on a mesh topology, especially for membrane proteins. This video accompanies the written discussion of the same topics ...

Mask Creation in ChimeraX for Cryo-EM Processing

21:22

Mask Creation in ChimeraX for Cryo-EM Processing

Просмотров 4,6 тыс.7 месяцев назад

In this video tutorial, we cover the general process of creating a mask in ChimeraX using three different techniques: volume segmentation, volume eraser, and molmap. This video accompanies the written guide for the same processes: guide.cryosparc.com/processing-data/tutorials-and-case-studies/mask-selection-and-generation-in-ucsf-chimera Chapters 00:00 Introduction 01:52 Pre-masking Volume Prep...

13:23

CryoSPARC v4.4: Workflows

Просмотров 1,2 тыс.10 месяцев назад

Workflows are a new system in CryoSPARC for quickly and easily populating a workspace with pre-defined sets of jobs. The system is designed with flexibility at its core. This means that it can be used to construct a top to bottom automated pipeline of jobs that can take you from import to refinement, a predefined branch of jobs for exploratory processing, or an arbitrary set of disconnected job...

CryoSPARC v4.4: Reference Based Motion Correction

17:22

CryoSPARC v4.4: Reference Based Motion Correction

Просмотров 2,8 тыс.10 месяцев назад

CryoSPARC v4.4 includes a new motion correction job, called “Reference-Based Motion Correction”, which uses a 3D map as a reference to improve per-particle motion estimates. Reference-based motion correction is an extension of patch motion correction. Using known particle poses and positions, precise movement trajectories can be calculated for each particle. In addition, the effect of radiation...

CryoSPARC 3D Flexible Refinement Tutorial

1:22:11

CryoSPARC 3D Flexible Refinement Tutorial

Просмотров 12 тыс.Год назад

3D Flexible Refinement (3DFlex) is a motion-based deep generative model for continuous heterogeneity. It can model non-rigid motion and flexibility of a protein molecule across its conformational landscape, and can use the motion model to combine signal from particle images in different conformations to improve refinement resolution in flexible regions. Learn more: guide.cryosparc.com/processin...

CryoSPARC 3D Flexible Refinement Mesh Preparation Tutorial

28:08

CryoSPARC 3D Flexible Refinement Mesh Preparation Tutorial

Просмотров 3,8 тыс.Год назад

20:46

CryoSPARC v4.0 Application Walkthrough

Просмотров 6 тыс.Год назад

We are pleased to announce that CryoSPARC v4.0 Beta is now available! The new version features an all-new interface designed for performance and productivity, alongside updates to software features and processing jobs. Check out the detailed changelog here: cryosparc.com/updates CryoSPARC v4.0 is a major upgrade. Please follow our Guide: Updating to CryoSPARC v4.0. Redesigned interactive jobs (...

CryoSPARC Live Tip: Test Particle Picking Adjustments

1:09

CryoSPARC Live Tip: Test Particle Picking Adjustments

Просмотров 1,4 тыс.3 года назад

Learn more about cryoSPARC Live here: guide.cryosparc.com/live/about-cryosparc-live

Real-time cryo-EM analysis for all: cryoSPARC Live

1:06:20

Real-time cryo-EM analysis for all: cryoSPARC Live

Просмотров 1,8 тыс.3 года назад

Learn how cryoSPARC Live, a seamless real-time 2D and 3D processing system for single particle cryo-EM, accelerates time-to-structure and drives rapid insights into sample characteristics and data quality, enabling decision making while the sample is still in the microscope. CryoSPARC Live is not just for facilities; it is also the fastest, simplest way for beginners and experts alike to proces...

10:01

cryoSPARC Live: 10-Minute Overview

Просмотров 1,4 тыс.3 года назад

To learn more about cryoSPARC Live, visit cryosparc.com/live

20:19

CryoSPARC Live Walkthrough

Просмотров 6 тыс.3 года назад

Watch the cryoSPARC v3.0 & cryoSPARC Live Launch Video: ruclips.net/video/QmjLOqZPCBs/видео.html Join us while we process cryo-EM data (EMPIAR-10288) using cryoSPARC Live, an all-new cryo-EM single particle analysis software system built by Structura Biotechnology. We'll walk you through the entire interface, and process this dataset from start to finish. CryoSPARC Live is available with the cr...

Introducing cryoSPARC v3.0 & the new and improved cryoSPARC Live!

1:11

Introducing cryoSPARC v3.0 & the new and improved cryoSPARC Live!

Просмотров 2 тыс.3 года назад

Watch us process cryo-EM data with the new cryoSPARC Live application from start to finish: ruclips.net/video/lrIHBb7Dr8w/видео.html CryoSPARC is a state of the art HPC software solution for complete processing of single-particle cryo-electron microscopy (cryo-EM) data. CryoSPARC Live is an all-new cryo-EM single particle analysis software system for real-time processing of raw microscope data ...

Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis

1:28:55

Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis

Просмотров 8 тыс.4 года назад

Learn how the new 3D Variability Analysis (3DVA) algorithm in cryoSPARC can reveal new functional and biological insight into the conformational and flexible motion dynamics of a protein molecule from single particle cryo-EM data. We will cover the new concepts introduced by the algorithm, interpretation of results, several case studies and examples, practical considerations to keep in mind whe...