Thank you for watching! did you find it helpful? Let me know in the comments. ▶ LinkedIn: www.linkedin.com/in/ahmedsaadfse/ ▶ Instagram: instagram.com/ai.techie/ ▶ Facebook: facebook.com/ai.techie1
Your videos are really helpful, please give voice over that would be a great help to the freshers and do more videos on empower on differenet topics with explanation like assay by hplc, dissolution by hplc, impurities by hplc, UOD by hplc and residual solvents by GC etc with broader explanation
@@AhmedSaadAE I will need a extense tutorial of everything I don't have a especific topic because I graduated recently and in all the jobs I have to use this program and I don't know so I would like to know the main thing to be able to work like in these last videos. Thanks to share this info
Thanks! We have E3 FR1; Do you have a tutorial on the use of reference curve? it is for Impurities calculation, but our version does not have the Impurities tab in the PM/...
Normally the Missing vial error should come form hardware side, like if Alliance system detects no vial it triggers the software to stops the Sample Set.
Hi, I want to created a processing method for Head space GC. We are using empower 3. I have a sample set where I will run system suitability test, samples and standards. However, I have Internal standard in all my samples. The method describes to calculate the concentration based on: ppm = (Rc/Rs) x Ps/Pc x dilution factor Where: Rc = ratio of component peak area and internal standard peak area in sample solution Rs = ratio of component peak area and internal standard peak area in standard solution Ps = quantity of solvent in standard preparation Pc = quantity of sample weighed I can add PS and PC and dilution factor in alter sample. However, how can I set up the processing method so that it will used Rc and Rs to calculate the concentration? Which means that it calculates based on Interanl standard in sample and in standard solution. Thanks for you help! Regards
Hi, Muhammad Mohsin, can you reach me at a.saad@aitechie.net As you need to have the custom field setup for concentration calculation. so we can discuss this further via email.
Hi, I'm working in an MNC company and in my project we are working using this empower software. Can you tell me the scope of this software? Will experience in this software help me in my career?
Column washing is normally depend on your column chemistry. In General if you are running Buffers as your mobile phase, you should wash the column with H2O first for alteast 20mins and than 40 to 50mins with organic solvent. Same goes with HPLC.
Linearity curve for the same concentration or multiple concentrations? Are you asking about the %deviation of the curve or Standard deviation of replicate injections?
Hello, very good videos, they are very helpful. I have just started using gas chromatography with empower, previously I used it with other software, do you think you could attach an example of how to load the cormatographic conditions and how to process data with an internal standard. It would help me a lot and I will appreciate it.
Thanks Daniel, Loading chromatographic condition to instrument via Empower is very simple as the instrument Method Editor UI is same as the Agilent/Shimadzu software. BTW which GC model you are using. Kindly share your Email so i can help you with Internal Standard.
Check for any leak first or air bubble in the lines, if its fine than it could be due to week Check Valves or Bad pump seals. You can try sonicating the Check Valve cartridges to see if this solve your problem.
To clear any previously saved calibration curve from the processing method. If you are calculating the unknown amount with pre-saved PM and putting the standard amount in either a sampler set or processing method then you can use this option. Hope I have cleared my point
Thank you for watching! did you find it helpful? Let me know in the comments.
▶ LinkedIn: www.linkedin.com/in/ahmedsaadfse/
▶ Instagram: instagram.com/ai.techie/
▶ Facebook: facebook.com/ai.techie1
Your videos are really helpful, please give voice over that would be a great help to the freshers and do more videos on empower on differenet topics with explanation like assay by hplc, dissolution by hplc, impurities by hplc, UOD by hplc and residual solvents by GC etc with broader explanation
Yes I am planning to add more video, starting from custom field calculation of Assay on Empower and with Voice :D
I don't have experience and I need more videos like this thanks
Hi Alex can you specify the Topic. I,ll try to make the same
@@AhmedSaadAE I will need a extense tutorial of everything I don't have a especific topic because I graduated recently and in all the jobs I have to use this program and I don't know so I would like to know the main thing to be able to work like in these last videos. Thanks to share this info
Thanks for sharing Empower 3 information, it really help me to handle LC easily..
Make video on peak purity, recording spectra in 3D by Empower.
Helpful video for empower user, Thanks. If it will possible make some video on empower.
Good job 👍 sir..
Thank you for sharing, very helpful, If you could make more videos like these, it would be great
Jazak Allah kaseera janab
Good Job Saad
Very informative keep growing .
Thanks! We have E3 FR1; Do you have a tutorial on the use of reference curve? it is for Impurities calculation, but our version does not have the Impurities tab in the PM/...
Very clarifying! Do I have to do all this same process to all my signals or is there a way to process the same method to other injections?
Yes you can process with the same method as far as your RT is same & consistent for your required Peak as defined in the processing method
This is very helpful, thank you
تسلم لو تكمل جميلك وتشرح لنا طريقة عمل كليبريشن واستدعاءه وطريقة تنظيمه وحذف بعض نقاطه
if you can comment in English, hopefully i can help
It’s very useful but if u have any vedio related tip and tricks in empower software
well currently not, but if you need any specific topic , i can record for you.
Good work
Hi sir, any control for missing vials?
Normally the Missing vial error should come form hardware side, like if Alliance system detects no vial it triggers the software to stops the Sample Set.
Hi, I want to created a processing method for Head space GC. We are using empower 3. I have a sample set where I will run system suitability test, samples and standards. However, I have Internal standard in all my samples. The method describes to calculate the concentration based on:
ppm = (Rc/Rs) x Ps/Pc x dilution factor
Where:
Rc = ratio of component peak area and internal standard peak area in sample solution
Rs = ratio of component peak area and internal standard peak area in standard solution
Ps = quantity of solvent in standard preparation
Pc = quantity of sample weighed
I can add PS and PC and dilution factor in alter sample. However, how can I set up the processing method so that it will used Rc and Rs to calculate the concentration? Which means that it calculates based on Interanl standard in sample and in standard solution.
Thanks for you help!
Regards
Hi,
Muhammad Mohsin, can you reach me at a.saad@aitechie.net
As you need to have the custom field setup for concentration calculation. so we can discuss this further via email.
Hi, I'm working in an MNC company and in my project we are working using this empower software. Can you tell me the scope of this software? Will experience in this software help me in my career?
You have any other video... Than share.... Because it's have no volume
Kindly elaborate column washing and routine washing for alience and equity
Column washing is normally depend on your column chemistry.
In General if you are running Buffers as your mobile phase, you should wash the column with H2O first for alteast 20mins and than 40 to 50mins with organic solvent.
Same goes with HPLC.
Make on Linearity curve and standard deviation curve ? Is it possible in empower?
Linearity curve for the same concentration or multiple concentrations?
Are you asking about the %deviation of the curve or Standard deviation of replicate injections?
Hello, very good videos, they are very helpful. I have just started using gas chromatography with empower, previously I used it with other software, do you think you could attach an example of how to load the cormatographic conditions and how to process data with an internal standard.
It would help me a lot and I will appreciate it.
Thanks Daniel,
Loading chromatographic condition to instrument via Empower is very simple as the instrument Method Editor UI is same as the Agilent/Shimadzu software. BTW which GC model you are using.
Kindly share your Email so i can help you with Internal Standard.
Please tell me about auto integration sir
Pressure fluctuations in system, resolved the solution
Check for any leak first or air bubble in the lines, if its fine than it could be due to week Check Valves or Bad pump seals.
You can try sonicating the Check Valve cartridges to see if this solve your problem.
Great...add voice record please
Planning for this.
great
Sir inka videos petaraa
Process krte hue ye clear calibration kyu krte h
To clear any previously saved calibration curve from the processing method.
If you are calculating the unknown amount with pre-saved PM and putting the standard amount in either a sampler set or processing method then you can use this option.
Hope I have cleared my point
There's no sound
Planning to release with sound soon. stay Tunned